Aqueous Extracts of the Marine Brown Alga Lobophora variegata Inhibit HIV-1 Infection at the Level of Virus Entry into Cells
Voolstra, Christian R.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Marine Science Program
Red Sea Research Center (RSRC)
Reef Genomics Lab
Permanent link to this recordhttp://hdl.handle.net/10754/334578
MetadataShow full item record
AbstractIn recent years, marine algae have emerged as a rich and promising source of molecules with potent activities against various human pathogens. The widely distributed brown alga Lobophora variegata that is often associated with tropical coral reefs exerts strong antibacterial and antiprotozoal effects, but so far has not been associated with specific anti-viral activities. This study investigated potential HIV-1 inhibitory activity of L. variegata collected from different geographical regions, using a cell-based full replication HIV-1 reporter assay. Aqueous L. variegata extracts showed strong inhibitory effects on several HIV-1 strains, including drug-resistant and primary HIV-1 isolates, and protected even primary cells (PBMC) from HIV-1-infection. Anti-viral potency was related to ecological factors and showed clear differences depending on light exposition or epiphyte growth. Assays addressing early events of the HIV-1 replication cycle indicated that L. variegata extracts inhibited entry of HIV-1 into cells at a pre-fusion step possibly by impeding mobility of virus particles. Further characterization of the aqueous extract demonstrated that even high doses had only moderate effects on viability of cultured and primary cells (PBMCs). Imaging-based techniques revealed extract effects on the plasma membrane and actin filaments as well as induction of apoptosis at concentrations exceeding EC50 of anti-HIV-1 activity by more than 400 fold. In summary, we show for the first time that L. variegata extracts inhibit HIV-1 entry, thereby suggesting this alga as promising source for the development of novel HIV-1 inhibitors.
CitationKremb S, Helfer M, Kraus B, Wolff H, Wild C, et al. (2014) Aqueous Extracts of the Marine Brown Alga Lobophora variegata Inhibit HIV-1 Infection at the Level of Virus Entry into Cells. PLoS ONE 9: e103895. doi:10.1371/journal.pone.0103895.
PublisherPublic Library of Science (PLoS)
PubMed Central IDPMC4140698
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Old plants newly discovered: Cassia sieberiana D.C. and Cassia abbreviata Oliv. Oliv. root extracts inhibit in vitro HIV-1c replication in peripheral blood mononuclear cells (PBMCs) by different modes of action.
- Authors: Leteane MM, Ngwenya BN, Muzila M, Namushe A, Mwinga J, Musonda R, Moyo S, Mengestu YB, Abegaz BM, Andrae-Marobela K
- Issue date: 2012 May 7
- The tropical brown alga Lobophora variegata: a source of antiprotozoal compounds.
- Authors: Cantillo-Ciau Z, Moo-Puc R, Quijano L, Freile-Pelegrín Y
- Issue date: 2010 Apr 16
- Aqueous extracts from peppermint, sage and lemon balm leaves display potent anti-HIV-1 activity by increasing the virion density.
- Authors: Geuenich S, Goffinet C, Venzke S, Nolkemper S, Baumann I, Plinkert P, Reichling J, Keppler OT
- Issue date: 2008 Mar 20
- Inhibition of HIV-1 infection by aqueous extracts of Prunella vulgaris L.
- Authors: Oh C, Price J, Brindley MA, Widrlechner MP, Qu L, McCoy JA, Murphy P, Hauck C, Maury W
- Issue date: 2011 Apr 23
- Antileishmanial properties of tropical marine algae extracts.
- Authors: Freile-Pelegrin Y, Robledo D, Chan-Bacab MJ, Ortega-Morales BO
- Issue date: 2008 Jul