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dc.contributor.authorJiang, Caifu
dc.contributor.authorMithani, Aziz
dc.contributor.authorGan, Xiangchao
dc.contributor.authorBelfield, Eric J.
dc.contributor.authorKlingler, John P.
dc.contributor.authorZhu, Jian-Kang
dc.contributor.authorRagoussis, Jiannis
dc.contributor.authorMott, Richard
dc.contributor.authorHarberd, Nicholas P.
dc.date.accessioned2014-11-11T14:30:04Z
dc.date.available2014-11-11T14:30:04Z
dc.date.issued2011-07-28
dc.identifier.citationJiang C, Mithani A, Gan X, Belfield EJ, Klingler JP, et al. (2011) Regenerant Arabidopsis Lineages Display a Distinct Genome-Wide Spectrum of Mutations Conferring Variant Phenotypes. Current Biology 21: 1385-1390. doi:10.1016/j.cub.2011.07.002.
dc.identifier.issn09609822
dc.identifier.pmid21802297
dc.identifier.doi10.1016/j.cub.2011.07.002
dc.identifier.urihttp://hdl.handle.net/10754/334570
dc.description.abstractMulticellular organisms can be regenerated from totipotent differentiated somatic cell or nuclear founders [1-3]. Organisms regenerated from clonally related isogenic founders might a priori have been expected to be phenotypically invariant. However, clonal regenerant animals display variant phenotypes caused by defective epigenetic reprogramming of gene expression [2], and clonal regenerant plants exhibit poorly understood heritable phenotypic ("somaclonal") variation [4-7]. Here we show that somaclonal variation in regenerant Arabidopsis lineages is associated with genome-wide elevation in DNA sequence mutation rate. We also show that regenerant mutations comprise a distinctive molecular spectrum of base substitutions, insertions, and deletions that probably results from decreased DNA repair fidelity. Finally, we show that while regenerant base substitutions are a likely major genetic cause of the somaclonal variation of regenerant Arabidopsis lineages, transposon movement is unlikely to contribute substantially to that variation. We conclude that the phenotypic variation of regenerant plants, unlike that of regenerant animals, is substantially due to DNA sequence mutation. 2011 Elsevier Ltd. All rights reserved.
dc.language.isoen
dc.publisherElsevier BV
dc.rightsOpen Access funded by Wellcome Trust
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectArabidopsis
dc.subjectgenetics
dc.subjecthistology
dc.subjectmolecular genetics
dc.subjectmutation
dc.subjectmutation rate
dc.subjectnucleotide sequence
dc.subjectphenotype
dc.subjectphysiology
dc.subjectplant genome
dc.subjectregeneration
dc.subjectsequence alignment
dc.subjecttransposon
dc.subjectArabidopsis
dc.subjectBase Sequence
dc.subjectDNA Mutational Analysis
dc.subjectDNA Transposable Elements
dc.subjectGenome, Plant
dc.subjectMolecular Sequence Data
dc.subjectMutation
dc.subjectMutation Rate
dc.subjectPhenotype
dc.subjectRegeneration
dc.subjectSequence Alignment
dc.subjectAnimalia
dc.subjectArabidopsis
dc.titleRegenerant arabidopsis lineages display a distinct genome-wide spectrum of mutations conferring variant phenotypes
dc.typeArticle
dc.contributor.departmentPlant Stress Genomics Research Lab
dc.identifier.journalCurrent Biology
dc.identifier.pmcidPMC3162137
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, United Kingdom
dc.contributor.institutionWellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, United Kingdom
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personKlingler, John
kaust.personZhu, Jian-Kang
refterms.dateFOA2018-06-14T04:38:26Z


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Open Access funded by Wellcome Trust
Except where otherwise noted, this item's license is described as Open Access funded by Wellcome Trust