Regenerant arabidopsis lineages display a distinct genome-wide spectrum of mutations conferring variant phenotypes
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ArticleAuthors
Jiang, CaifuMithani, Aziz
Gan, Xiangchao
Belfield, Eric J.
Klingler, John

Zhu, Jian-Kang

Ragoussis, Jiannis
Mott, Richard
Harberd, Nicholas P.
KAUST Department
Center for Desert AgricultureCompetitive Research Funds
GCR - New Progs, Collab & PreAward Admin
Plant Stress Genomics Research Lab
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2011-08-02Online Publication Date
2011-08-02Print Publication Date
2011-08Permanent link to this record
http://hdl.handle.net/10754/334570
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Multicellular organisms can be regenerated from totipotent differentiated somatic cell or nuclear founders [1-3]. Organisms regenerated from clonally related isogenic founders might a priori have been expected to be phenotypically invariant. However, clonal regenerant animals display variant phenotypes caused by defective epigenetic reprogramming of gene expression [2], and clonal regenerant plants exhibit poorly understood heritable phenotypic ("somaclonal") variation [4-7]. Here we show that somaclonal variation in regenerant Arabidopsis lineages is associated with genome-wide elevation in DNA sequence mutation rate. We also show that regenerant mutations comprise a distinctive molecular spectrum of base substitutions, insertions, and deletions that probably results from decreased DNA repair fidelity. Finally, we show that while regenerant base substitutions are a likely major genetic cause of the somaclonal variation of regenerant Arabidopsis lineages, transposon movement is unlikely to contribute substantially to that variation. We conclude that the phenotypic variation of regenerant plants, unlike that of regenerant animals, is substantially due to DNA sequence mutation. 2011 Elsevier Ltd. All rights reserved.Citation
Jiang C, Mithani A, Gan X, Belfield EJ, Klingler JP, et al. (2011) Regenerant Arabidopsis Lineages Display a Distinct Genome-Wide Spectrum of Mutations Conferring Variant Phenotypes. Current Biology 21: 1385-1390. doi:10.1016/j.cub.2011.07.002.Publisher
Elsevier BVJournal
Current BiologyPubMed ID
21802297PubMed Central ID
PMC3162137ae974a485f413a2113503eed53cd6c53
10.1016/j.cub.2011.07.002
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Except where otherwise noted, this item's license is described as Open Access funded by Wellcome Trust
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