Show simple item record

dc.contributor.authorTewari, Rita
dc.contributor.authorStraschil, Ursula
dc.contributor.authorBateman, Alex
dc.contributor.authorBöhme, Ulrike
dc.contributor.authorCherevach, Inna
dc.contributor.authorGong, Peng
dc.contributor.authorPain, Arnab
dc.contributor.authorBillker, Oliver
dc.date.accessioned2014-11-11T14:30:01Z
dc.date.available2014-11-11T14:30:01Z
dc.date.issued2010-10-21
dc.identifier.citationTewari R, Straschil U, Bateman A, Böhme U, Cherevach I, et al. (2010) The Systematic Functional Analysis of Plasmodium Protein Kinases Identifies Essential Regulators of Mosquito Transmission. Cell Host and Microbe 8: 377-387. doi:10.1016/j.chom.2010.09.006.
dc.identifier.issn19313128
dc.identifier.pmid20951971
dc.identifier.doi10.1016/j.chom.2010.09.006
dc.identifier.urihttp://hdl.handle.net/10754/334569
dc.description.abstractAlthough eukaryotic protein kinases (ePKs) contribute to many cellular processes, only three Plasmodium falciparum ePKs have thus far been identified as essential for parasite asexual blood stage development. To identify pathways essential for parasite transmission between their mammalian host and mosquito vector, we undertook a systematic functional analysis of ePKs in the genetically tractable rodent parasite Plasmodium berghei. Modeling domain signatures of conventional ePKs identified 66 putative Plasmodium ePKs. Kinomes are highly conserved between Plasmodium species. Using reverse genetics, we show that 23 ePKs are redundant for asexual erythrocytic parasite development in mice. Phenotyping mutants at four life cycle stages in Anopheles stephensi mosquitoes revealed functional clusters of kinases required for sexual development and sporogony. Roles for a putative SR protein kinase (SRPK) in microgamete formation, a conserved regulator of clathrin uncoating (GAK) in ookinete formation, and a likely regulator of energy metabolism (SNF1/KIN) in sporozoite development were identified. 2010 Elsevier Inc.
dc.language.isoen
dc.publisherElsevier BV
dc.rightsOpen Access funded by Wellcome Trust
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectclathrin
dc.subjectprotein kinase
dc.subjectenergy metabolism
dc.subjectmosquito
dc.subjectnonhuman
dc.subjectparasite development
dc.subjectphenotype
dc.subjectPlasmodium falciparum
dc.subjectpriority journal
dc.subjectprotein structure
dc.subjectsalivary gland
dc.subjectsexual development
dc.subjectsporozoite
dc.subjectzygote
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectAnopheles
dc.subjectEnergy Metabolism
dc.subjectFemale
dc.subjectGene Deletion
dc.subjectGene Knockout Techniques
dc.subjectInsect Vectors
dc.subjectLife Cycle Stages
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectMolecular Sequence Data
dc.subjectMutation
dc.subjectPlasmodium berghei
dc.subjectPlasmodium falciparum
dc.subjectProtein Kinases
dc.subjectProtozoan Proteins
dc.subjectSporozoites
dc.subjectAnopheles stephensi
dc.subjectEukaryota
dc.subjectMammalia
dc.subjectMus
dc.subjectPlasmodium (Apicomplexa)
dc.subjectPlasmodium berghei
dc.subjectPlasmodium falciparum
dc.subjectRodentia
dc.titleThe systematic functional analysis of plasmodium protein kinases identifies essential regulators of mosquito transmission
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentPathogen Genomics Laboratory
dc.identifier.journalCell Host & Microbe
dc.identifier.pmcidPMC2977076
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstitute of Genetics, QMC, University of Nottingham, Nottingham NG7 2UH, United Kingdom
dc.contributor.institutionDivision of Cell and Molecular Biology, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom
dc.contributor.institutionWellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personPain, Arnab
refterms.dateFOA2018-06-14T04:38:25Z
dc.date.published-online2010-10-21
dc.date.published-print2010-10


Files in this item

Thumbnail
Name:
Article-Cell_Host_-The_system-2010.pdf
Size:
1.358Mb
Format:
PDF
Description:
Article - Full Text

This item appears in the following Collection(s)

Show simple item record

Open Access funded by Wellcome Trust
Except where otherwise noted, this item's license is described as Open Access funded by Wellcome Trust