Timing, coordination, and rhythm: Acrobatics at the DNA replication fork
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Laboratory of DNA Replication and Recombination
Online Publication Date2010-04-09
Print Publication Date2010-06-18
Permanent link to this recordhttp://hdl.handle.net/10754/334568
MetadataShow full item record
AbstractIn DNA replication, the antiparallel nature of the parental duplex imposes certain constraints on the activity of the DNA polymerases that synthesize new DNA. The leading-strand polymerase advances in a continuous fashion, but the lagging-strand polymerase is forced to restart at short intervals. In several prokaryotic systems studied so far, this problem is solved by the formation of a loop in the lagging strand of the replication fork to reorient the lagging-strand DNA polymerase so that it advances in parallel with the leading-strand polymerase. The replication loop grows and shrinks during each cycle of Okazaki fragment synthesis. The timing of Okazaki fragment synthesis and loop formation is determined by a subtle interplay of enzymatic activities at the fork. Recent developments in single-molecule techniques have enabled the direct observation of these processes and have greatly contributed to a better understanding of the dynamic nature of the replication fork. Here, we will review recent experimental advances, present the current models, and discuss some of the exciting developments in the field. 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
CitationHamdan SM, van Oijen AM (2010) Timing, Coordination, and Rhythm: Acrobatics at the DNA Replication Fork. Journal of Biological Chemistry 285: 18979-18983. doi:10.1074/jbc.R109.022939.
JournalJournal of Biological Chemistry
PubMed Central IDPMC2885174
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Except where otherwise noted, this item's license is described as Author's Choice—Final version full access. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles
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- Authors: Zhang H, Tang Y, Lee SJ, Wei Z, Cao J, Richardson CC
- Issue date: 2016 Jan 15
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