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dc.contributor.authorGiles, Emily
dc.contributor.authorSaenz Agudelo, Pablo
dc.contributor.authorBerumen, Michael L.
dc.contributor.authorRavasi, Timothy
dc.date.accessioned2014-11-11T14:27:55Z
dc.date.available2014-11-11T14:27:55Z
dc.date.issued2013-04-04
dc.identifier.citationGiles EC, Saenz-Agudelo P, Berumen ML, Ravasi T (2013) Novel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carteri. Marine Biodiversity 43: 237-241. doi:10.1007/s12526-013-0151-x.
dc.identifier.issn18671616
dc.identifier.doi10.1007/s12526-013-0151-x
dc.identifier.urihttp://hdl.handle.net/10754/334514
dc.description.abstractDespite the ubiquitous role sponges play in reef ecosystem dynamics, little is known about population-level connectivity in these organisms. The general field of population genetics in sponges remains in its infancy. To date, microsatellite markers have only been developed for few sponge species and no sponge population genetics studies using microsatellites have been conducted in the Red Sea. Here, with the use of next-generation sequencing, we characterize 12 novel polymorphic loci for the common reef sponge, Stylissa carteri. The number of alleles per loci ranged between three and eight. Observed heterozygosity frequencies (Ho) ranged from 0.125 to 0.870, whereas expected (He) heterozygosity frequencies ranged from 0.119 to 0.812. Only one locus showed consistent deviations from Hardy-Weinberg equilibrium (HWE) in both populations and two loci consistently showed the possible presence of null alleles. No significant linkage disequilibrium was detected for any pairs of loci. These microsatellites will be of use for numerous ecological studies focused on this common and abundant sponge. 2013 The Author(s).
dc.language.isoen
dc.publisherSpringer Nature
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectConnectivity
dc.subjectMicrosatellites
dc.subjectPopulation genetics
dc.subjectSponges
dc.subjectStylissa carteri
dc.subjectallele
dc.subjectconnectivity
dc.subjectevolutionary theory
dc.subjectgenetic analysis
dc.subjectgenetic marker
dc.subjectheterozygosity
dc.subjectpolymorphism
dc.subjectpopulation genetics
dc.subjectreef
dc.subjectsponge
dc.subjectIndian Ocean
dc.subjectRed Sea [Indian Ocean]
dc.titleNovel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carteri
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentIntegrative Systems Biology Lab
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentReef Ecology Lab
dc.identifier.journalMarine Biodiversity
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionBiology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, United States
dc.contributor.institutionDepartment of Medicine, Division of Medical Genetics, University of California, San Diego, 9500 Gilman Drive La Jolla, San Diego, CA, 92093, United States
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personGiles, Emily
kaust.personSaenz Agudelo, Pablo
kaust.personBerumen, Michael L.
kaust.personRavasi, Timothy
refterms.dateFOA2018-06-13T15:40:45Z
dc.date.published-online2013-04-04
dc.date.published-print2013-09


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