Novel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carteri
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Integrative Systems Biology Lab
Marine Science Program
Red Sea Research Center (RSRC)
Reef Ecology Lab
Online Publication Date2013-04-04
Print Publication Date2013-09
Permanent link to this recordhttp://hdl.handle.net/10754/334514
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AbstractDespite the ubiquitous role sponges play in reef ecosystem dynamics, little is known about population-level connectivity in these organisms. The general field of population genetics in sponges remains in its infancy. To date, microsatellite markers have only been developed for few sponge species and no sponge population genetics studies using microsatellites have been conducted in the Red Sea. Here, with the use of next-generation sequencing, we characterize 12 novel polymorphic loci for the common reef sponge, Stylissa carteri. The number of alleles per loci ranged between three and eight. Observed heterozygosity frequencies (Ho) ranged from 0.125 to 0.870, whereas expected (He) heterozygosity frequencies ranged from 0.119 to 0.812. Only one locus showed consistent deviations from Hardy-Weinberg equilibrium (HWE) in both populations and two loci consistently showed the possible presence of null alleles. No significant linkage disequilibrium was detected for any pairs of loci. These microsatellites will be of use for numerous ecological studies focused on this common and abundant sponge. 2013 The Author(s).
CitationGiles EC, Saenz-Agudelo P, Berumen ML, Ravasi T (2013) Novel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carteri. Marine Biodiversity 43: 237-241. doi:10.1007/s12526-013-0151-x.
CollectionsArticles; Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC); Bioscience Program; Marine Science Program; Integrative Systems Biology Lab; Computational Bioscience Research Center (CBRC); Reef Genomics, part of the Global Ocean Genome Project
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