Transcriptome analysis elucidates key developmental components of bryozoan lophophore development
AuthorsWong, Yue Him
Ryu, Tae Woo
KAUST DepartmentComputational Bioscience Research Center (CBRC)
Biological and Environmental Sciences and Engineering (BESE) Division
Applied Mathematics and Computational Science Program
Computer Science Program
Permanent link to this recordhttp://hdl.handle.net/10754/333562
MetadataShow full item record
AbstractThe most recent phylogenomic study suggested that Bryozoa (Ectoprocta), Brachiopoda, and Phoronida are monophyletic, implying that the lophophore of bryozoans, phoronids and brachiopods is a synapomorphy. Understanding the molecular mechanisms of the lophophore development of the Lophophorata clade can therefore provide us a new insight into the formation of the diverse morphological traits in metazoans. In the present study, we profiled the transcriptome of the Bryozoan (Ectoproct) Bugula neritina during the swimming larval stage (SW) and the early (4 h) and late (24 h) metamorphic stages using the Illumina HiSeq2000 platform. Various genes that function in development, the immune response and neurogenesis showed differential expression levels during metamorphosis. In situ hybridization of 23 genes that participate in the Wnt, BMP, Notch, and Hedgehog signaling pathways revealed their regulatory roles in the development of the lophophore and the ancestrula digestive tract. Our findings support the hypothesis that developmental precursors of the lophophore and the ancestrula digestive tract are pre-patterned by the differential expression of key developmental genes according to their fate. This study provides a foundation to better understand the developmental divergence and/or convergence among developmental precursors of the lophophore of bryozoans, branchiopods and phoronids.
CitationWong, Y. H., Ryu, T., Seridi, L., Ghosheh, Y., Bougouffa, S., Qian, P.-Y., & Ravasi, T. (2014). Transcriptome analysis elucidates key developmental components of bryozoan lophophore development. Sci. Rep., 4. doi: 10.1038/srep06534
SponsorsThis study was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, an award from King Abdullah University of Science and Technology [SA-C0040/UK-C0016], and grants from the Research Grants Council of the Hong Kong Special Administrative Region [GRF661611 and GRF662413] to Pei-Yuan Qian.
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
- Involvement of Wnt signaling pathways in the metamorphosis of the bryozoan Bugula neritina.
- Authors: Wong YH, Wang H, Ravasi T, Qian PY
- Issue date: 2012
- Complete mitochondrial genome of Bugula neritina (Bryozoa, Gymnolaemata, Cheilostomata): phylogenetic position of Bryozoa and phylogeny of lophophorates within the Lophotrochozoa.
- Authors: Jang KH, Hwang UW
- Issue date: 2009 Apr 21
- New phylogenomic data support the monophyly of Lophophorata and an Ectoproct-Phoronid clade and indicate that Polyzoa and Kryptrochozoa are caused by systematic bias.
- Authors: Nesnidal MP, Helmkampf M, Meyer A, Witek A, Bruchhaus I, Ebersberger I, Hankeln T, Lieb B, Struck TH, Hausdorf B
- Issue date: 2013 Nov 17
- Modern Data on the Innervation of the Lophophore in Lingula anatina (Brachiopoda) Support the Monophyly of the Lophophorates.
- Authors: Temereva EN, Tsitrin EB
- Issue date: 2015
- Evidence from Hox genes that bryozoans are lophotrochozoans.
- Authors: Passamaneck YJ, Halanych KM
- Issue date: 2004 Jul-Aug