Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of theileria-induced leukocyte transformation
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Type
ArticleAuthors
Hayashida, KyokoHara, Yuichiro
Abe, Takashi
Yamasaki, Chisato
Toyoda, Atsushi
Kosuge, Takehide
Suzuki, Yutaka
Sato, Yoshiharu
Kawashima, Shuichi
Katayama, Toshiaki
Wakaguri, Hiroyuki
Inoue, Noboru
Homma, Keiichi
Tada-Umezaki, Masahito
Yagi, Yukio
Fujii, Yasuyuki
Habara, Takuya
Kanehisa, Minoru
Watanabe, Hidemi
Ito, Kimihito
Gojobori, Takashi
Sugawara, Hideaki
Imanishi, Tadashi
Weir, William
Gardner, Malcolm
Pain, Arnab
Shiels, Brian
Hattori, Masahira
Nene, Vishvanath
Sugimoto, Chihiro
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Computational Bioscience Research Center (CBRC)
Pathogen Genomics Laboratory
Date
2012-09-04Online Publication Date
2012-09-04Print Publication Date
2012-09-04Permanent link to this record
http://hdl.handle.net/10754/325462Metadata
Show full item recordAbstract
We sequenced the genome of Theileria orientalis, a tick-borne apicomplexan protozoan parasite of cattle. The focus of this study was a comparative genome analysis of T. orientalis relative to other highly pathogenic Theileria species, T. parva and T. annulata. T. parva and T. annulata induce transformation of infected cells of lymphocyte or macrophage/monocyte lineages; in contrast, T. orientalis does not induce uncontrolled proliferation of infected leukocytes and multiplies predominantly within infected erythrocytes. While synteny across homologous chromosomes of the three Theileria species was found to be well conserved overall, subtelomeric structures were found to differ substantially, as T. orientalis lacks the large tandemly arrayed subtelomere-encoded variable secreted protein-encoding gene family. Moreover, expansion of particular gene families by gene duplication was found in the genomes of the two transforming Theileria species, most notably, the TashAT/TpHN and Tar/Tpr gene families. Gene families that are present only in T. parva and T. annulata and not in T. orientalis, Babesia bovis, or Plasmo-dium were also identified. Identification of differences between the genome sequences of Theileria species with different abilities to transform and immortalize bovine leukocytes will provide insight into proteins and mechanisms that have evolved to induce and regulate this process. The T. orientalis genome database is available at http://totdb.czc.hokudai.ac.jp/. 2012 Hayashida et al. T.Citation
Hayashida K, Hara Y, Abe T, Yamasaki C, Toyoda A, et al. (2012) Comparative Genome Analysis of Three Eukaryotic Parasites with Differing Abilities To Transform Leukocytes Reveals Key Mediators of Theileria-Induced Leukocyte Transformation. mBio 3: e00204-12-e00204-12. doi:10.1128/mBio.00204-12.Publisher
American Society for MicrobiologyJournal
mBioPubMed ID
22951932PubMed Central ID
PMC3445966Scopus Count
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