Dissecting the interactions of SERRATE with RNA and DICER-LIKE 1 in Arabidopsis microRNA precursor processing
KAUST DepartmentDesert Agriculture Initiative
Biological and Environmental Sciences and Engineering (BESE) Division
MetadataShow full item record
AbstractEfficient and precise microRNA (miRNA) biogenesis in Arabidopsis is mediated by the RNaseIII-family enzyme DICER-LIKE 1 (DCL1), double-stranded RNA-binding protein HYPONASTIC LEAVES 1 and the zinc-finger (ZnF) domain-containing protein SERRATE (SE). In the present study, we examined primary miRNA precursor (pri-miRNA) processing by highly purified recombinant DCL1 and SE proteins and found that SE is integral to pri-miRNA processing by DCL1. SE stimulates DCL1 cleavage of the pri-miRNA in an ionic strength-dependent manner. SE uses its N-terminal domain to bind to RNA and requires both N-terminal and ZnF domains to bind to DCL1. However, when DCL1 is bound to RNA, the interaction with the ZnF domain of SE becomes indispensible and stimulates the activity of DCL1 without requiring SE binding to RNA. Our results suggest that the interactions among SE, DCL1 and RNA are a potential point for regulating pri-miRNA processing. 2013 The Author(s) 2013.
CitationIwata Y, Takahashi M, Fedoroff NV, Hamdan SM (2013) Dissecting the interactions of SERRATE with RNA and DICER-LIKE 1 in Arabidopsis microRNA precursor processing. Nucleic Acids Research 41: 9129-9140. doi:10.1093/nar/gkt667.
PublisherOxford University Press (OUP)
JournalNucleic Acids Research
PubMed Central IDPMC3799435
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Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Molecular insights into miRNA processing by Arabidopsis thaliana SERRATE.
- Authors: Machida S, Chen HY, Adam Yuan Y
- Issue date: 2011 Sep 1
- The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1.
- Authors: Dong Z, Han MH, Fedoroff N
- Issue date: 2008 Jul 22
- Homodimerization of HYL1 ensures the correct selection of cleavage sites in primary miRNA.
- Authors: Yang X, Ren W, Zhao Q, Zhang P, Wu F, He Y
- Issue date: 2014 Oct 29
- Dual roles of the nuclear cap-binding complex and SERRATE in pre-mRNA splicing and microRNA processing in Arabidopsis thaliana.
- Authors: Laubinger S, Sachsenberg T, Zeller G, Busch W, Lohmann JU, Rätsch G, Weigel D
- Issue date: 2008 Jun 24
- Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants.
- Authors: Fang Y, Spector DL
- Issue date: 2007 May 1
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