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dc.contributor.authorRodrigo, María J.
dc.contributor.authorAlquézar, Berta
dc.contributor.authorAlós, Enriqueta
dc.contributor.authorMedina, Víctor
dc.contributor.authorCarmona, Lourdes
dc.contributor.authorBruno, Mark
dc.contributor.authorAl-Babili, Salim
dc.contributor.authorZacarías, Lorenzo
dc.date.accessioned2014-08-27T09:51:44Z
dc.date.available2014-08-27T09:51:44Z
dc.date.issued2013-09-04
dc.identifier.citationRodrigo MJ, Alquezar B, Alos E, Medina V, Carmona L, et al. (2013) A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments. Journal of Experimental Botany 64: 4461-4478. doi:10.1093/jxb/ert260.
dc.identifier.issn00220957
dc.identifier.pmid24006419
dc.identifier.doi10.1093/jxb/ert260
dc.identifier.urihttp://hdl.handle.net/10754/325447
dc.description.abstractCitrus is the first tree crop in terms of fruit production. The colour of Citrus fruit is one of the main quality attributes, caused by the accumulation of carotenoids and their derivative C30 apocarotenoids, mainly ?-citraurin (3-hydroxy-?-apo-8?-carotenal), which provide an attractive orange-reddish tint to the peel of oranges and mandarins. Though carotenoid biosynthesis and its regulation have been extensively studied in Citrus fruits, little is known about the formation of C30 apocarotenoids. The aim of this study was to the identify carotenoid cleavage enzyme(s) [CCD(s)] involved in the peel-specific C30 apocarotenoids. In silico data mining revealed a new family of five CCD4-type genes in Citrus. One gene of this family, CCD4b1, was expressed in reproductive and vegetative tissues of different Citrus species in a pattern correlating with the accumulation of C30 apocarotenoids. Moreover, developmental processes and treatments which alter Citrus fruit peel pigmentation led to changes of ?-citraurin content and CCD4b1 transcript levels. These results point to the involvement of CCD4b1 in ?-citraurin formation and indicate that the accumulation of this compound is determined by the availability of the presumed precursors zeaxanthin and ?-cryptoxanthin. Functional analysis of CCD4b1 by in vitro assays unequivocally demonstrated the asymmetric cleavage activity at the 7?,8? double bond in zeaxanthin and ?-cryptoxanthin, confrming its role in C30 apocarotenoid biosynthesis. Thus, a novel plant carotenoid cleavage activity targeting the 7?,8? double bond of cyclic C40 carotenoids has been identified. These results suggest that the presented enzyme is responsible for the biosynthesis of C30 apocarotenoids in Citrus which are key pigments in fruit coloration. The Author 2013.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/3.0
dc.subject?-citraurin
dc.subjectApocarotenoid
dc.subjectCarotenoid
dc.subjectCarotenoid cleavage dioxygenase
dc.subjectCitrus
dc.subjectFruit coloration
dc.subjectcarotenoid
dc.subjectethylene
dc.subjectethylene derivative
dc.subjectmessenger RNA
dc.subjectvegetable protein
dc.subjectamino acid sequence
dc.subjectantibody specificity
dc.subjectbiosynthesis
dc.subjectcarotenoid cleavage dioxygenase
dc.subjectchemical structure
dc.subjectchemistry
dc.subjectdrug effect
dc.subjectfruit coloration.
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectheat
dc.subjecthigh performance liquid chromatography
dc.subjectmetabolism
dc.subjectmolecular genetics
dc.subjectmultigene family
dc.subjectplant gene
dc.subjectreproduction
dc.subjectsequence alignment
dc.subjectcarotenoid
dc.subjectcarotenoid cleavage dioxygenase
dc.subjectfruit coloration.
dc.subjectAmino Acid Sequence
dc.subjectCarotenoids
dc.subjectChromatography, High Pressure Liquid
dc.subjectEthylenes
dc.subjectGene Expression Regulation, Plant
dc.subjectGenes, Plant
dc.subjectHot Temperature
dc.subjectModels, Molecular
dc.subjectMolecular Sequence Data
dc.subjectMultigene Family
dc.subjectOrgan Specificity
dc.subjectPlant Proteins
dc.subjectReproduction
dc.subjectRNA, Messenger
dc.subjectSequence Alignment
dc.titleA novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentPlant Science
dc.identifier.journalJournal of Experimental Botany
dc.identifier.pmcidPMC3808326
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstituto de Agroqumica y Tecnologa de Alimentos, Consejo Superior de Investigaciones Cientficas (IATA-CSIC), Av. Agustn Escardino 7, 46980 Paterna, Valencia, Spain
dc.contributor.institutionFaculty of Biology, University of Freiburg, D-79104 Freiburg, Germany
dc.contributor.institutionCentro de Proteccin Vegetal y Biotecnologa, Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada-Nquera, 46113, Moncada, Valencia, Spain
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personAl-Babili, Salim
refterms.dateFOA2018-06-13T15:25:25Z
dc.date.published-online2013-09-04
dc.date.published-print2013-11


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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.