YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana
dc.contributor.author | Kim, Jeong Im | |
dc.contributor.author | Murphy, Angus S. | |
dc.contributor.author | Baek, Dongwon | |
dc.contributor.author | Lee, Shin-Woo | |
dc.contributor.author | Yun, Dae-Jin | |
dc.contributor.author | Bressan, Ray Anthony | |
dc.contributor.author | Narasimhan, Meena L. | |
dc.date.accessioned | 2014-08-27T09:51:38Z | |
dc.date.available | 2014-08-27T09:51:38Z | |
dc.date.issued | 2011-04-21 | |
dc.identifier.citation | Kim JI, Murphy AS, Baek D, Lee S-W, Yun D-J, et al. (2011) YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana. Journal of Experimental Botany 62: 3981-3992. doi:10.1093/jxb/err094. | |
dc.identifier.issn | 00220957 | |
dc.identifier.pmid | 21511905 | |
dc.identifier.doi | 10.1093/jxb/err094 | |
dc.identifier.uri | http://hdl.handle.net/10754/325445 | |
dc.description.abstract | The Arabidopsis thaliana YUCCA family of flavin monooxygenase proteins catalyses a rate-limiting step in de novo auxin biosynthesis. A YUCCA6 activation mutant, yuc6-1D, has been shown to contain an elevated free IAA level and to display typical high-auxin phenotypes. It is reported here that Arabidopsis plants over-expressing YUCCA6, such as the yuc6-1D activation mutant and 35S:YUC6 transgenic plants, displayed dramatic longevity. In addition, plants over-expressing YUCCA6 exhibited classical, delayed dark-induced and hormone-induced senescence in assays using detached rosette leaves. However, plants over-expressing an allele of YUCCA6, that carries mutations in the NADPH cofactor binding site, exhibited neither delayed leaf senescence phenotypes nor phenotypes typical of auxin overproduction. When the level of free IAA was reduced in yuc6-1D by conjugation to lysine, yuc6-1D leaves senesced at a rate similar to the wild-type leaves. Dark-induced senescence in detached leaves was accompanied by a decrease in their free IAA content, by the reduced expression of auxin biosynthesis enzymes such as YUCCA1 and YUCCA6 that increase cellular free IAA levels, and by the increased expression of auxin-conjugating enzymes encoded by the GH3 genes that reduce the cellular free auxin levels. Reduced transcript abundances of SAG12, NAC1, and NAC6 during senescence in yuc6-1D compared with the wild type suggested that auxin delays senescence by directly or indirectly regulating the expression of senescence-associated genes. 2011 The Author(s). | |
dc.language.iso | en | |
dc.publisher | Oxford University Press (OUP) | |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details) | |
dc.subject | Arabidopsis thaliana | |
dc.subject | auxin | |
dc.subject | leaf senescence | |
dc.subject | longevity | |
dc.subject | YUCCA6 | |
dc.subject | Arabidopsis protein | |
dc.subject | indoleacetic acid | |
dc.subject | indoleacetic acid derivative | |
dc.subject | mixed function oxidase | |
dc.subject | phytohormone | |
dc.subject | YUCCA6 protein, Arabidopsis | |
dc.subject | Arabidopsis | |
dc.subject | dominant gene | |
dc.subject | gene expression regulation | |
dc.subject | genetics | |
dc.subject | growth, development and aging | |
dc.subject | metabolism | |
dc.subject | mutation | |
dc.subject | plant leaf | |
dc.subject | transgenic plant | |
dc.subject | Arabidopsis | |
dc.subject | Arabidopsis Proteins | |
dc.subject | Gene Expression Regulation, Plant | |
dc.subject | Genes, Dominant | |
dc.subject | Indoleacetic Acids | |
dc.subject | Mixed Function Oxygenases | |
dc.subject | Mutagenesis, Insertional | |
dc.subject | Mutation | |
dc.subject | Plant Growth Regulators | |
dc.subject | Plant Leaves | |
dc.subject | Plants, Genetically Modified | |
dc.subject | Arabidopsis | |
dc.title | YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana | |
dc.type | Article | |
dc.contributor.department | Biological and Environmental Sciences and Engineering (BESE) Division | |
dc.contributor.department | Desert Agriculture Initiative | |
dc.contributor.department | Plant Stress Genomics Research Lab | |
dc.identifier.journal | Journal of Experimental Botany | |
dc.identifier.pmcid | PMC3134353 | |
dc.eprint.version | Publisher's Version/PDF | |
dc.contributor.institution | Department of Biochemistry, Purdue University, West Lafayette, IN 47907-2010, United States | |
dc.contributor.institution | Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907-2010, United States | |
dc.contributor.institution | Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, South Korea | |
dc.contributor.institution | Department of Agronomy and Medicinal Plant Resources, Gyeongnam National University of Science and Technology, Jinju 660-758, South Korea | |
dc.contributor.affiliation | King Abdullah University of Science and Technology (KAUST) | |
kaust.person | Bressan, Ray Anthony | |
kaust.person | Narasimhan, Meena L. | |
refterms.dateFOA | 2018-06-14T04:31:50Z | |
dc.date.published-online | 2011-04-21 | |
dc.date.published-print | 2011-07 |