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dc.contributor.authorKim, Jeong Im
dc.contributor.authorMurphy, Angus S.
dc.contributor.authorBaek, Dongwon
dc.contributor.authorLee, Shin-Woo
dc.contributor.authorYun, Dae-Jin
dc.contributor.authorBressan, Ray Anthony
dc.contributor.authorNarasimhan, Meena L.
dc.date.accessioned2014-08-27T09:51:38Z
dc.date.available2014-08-27T09:51:38Z
dc.date.issued2011-04-21
dc.identifier.citationKim JI, Murphy AS, Baek D, Lee S-W, Yun D-J, et al. (2011) YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana. Journal of Experimental Botany 62: 3981-3992. doi:10.1093/jxb/err094.
dc.identifier.issn00220957
dc.identifier.pmid21511905
dc.identifier.doi10.1093/jxb/err094
dc.identifier.urihttp://hdl.handle.net/10754/325445
dc.description.abstractThe Arabidopsis thaliana YUCCA family of flavin monooxygenase proteins catalyses a rate-limiting step in de novo auxin biosynthesis. A YUCCA6 activation mutant, yuc6-1D, has been shown to contain an elevated free IAA level and to display typical high-auxin phenotypes. It is reported here that Arabidopsis plants over-expressing YUCCA6, such as the yuc6-1D activation mutant and 35S:YUC6 transgenic plants, displayed dramatic longevity. In addition, plants over-expressing YUCCA6 exhibited classical, delayed dark-induced and hormone-induced senescence in assays using detached rosette leaves. However, plants over-expressing an allele of YUCCA6, that carries mutations in the NADPH cofactor binding site, exhibited neither delayed leaf senescence phenotypes nor phenotypes typical of auxin overproduction. When the level of free IAA was reduced in yuc6-1D by conjugation to lysine, yuc6-1D leaves senesced at a rate similar to the wild-type leaves. Dark-induced senescence in detached leaves was accompanied by a decrease in their free IAA content, by the reduced expression of auxin biosynthesis enzymes such as YUCCA1 and YUCCA6 that increase cellular free IAA levels, and by the increased expression of auxin-conjugating enzymes encoded by the GH3 genes that reduce the cellular free auxin levels. Reduced transcript abundances of SAG12, NAC1, and NAC6 during senescence in yuc6-1D compared with the wild type suggested that auxin delays senescence by directly or indirectly regulating the expression of senescence-associated genes. 2011 The Author(s).
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
dc.subjectArabidopsis thaliana
dc.subjectauxin
dc.subjectleaf senescence
dc.subjectlongevity
dc.subjectYUCCA6
dc.subjectArabidopsis protein
dc.subjectindoleacetic acid
dc.subjectindoleacetic acid derivative
dc.subjectmixed function oxidase
dc.subjectphytohormone
dc.subjectYUCCA6 protein, Arabidopsis
dc.subjectArabidopsis
dc.subjectdominant gene
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjectmetabolism
dc.subjectmutation
dc.subjectplant leaf
dc.subjecttransgenic plant
dc.subjectArabidopsis
dc.subjectArabidopsis Proteins
dc.subjectGene Expression Regulation, Plant
dc.subjectGenes, Dominant
dc.subjectIndoleacetic Acids
dc.subjectMixed Function Oxygenases
dc.subjectMutagenesis, Insertional
dc.subjectMutation
dc.subjectPlant Growth Regulators
dc.subjectPlant Leaves
dc.subjectPlants, Genetically Modified
dc.subjectArabidopsis
dc.titleYUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentPlant Stress Genomics Research Lab
dc.identifier.journalJournal of Experimental Botany
dc.identifier.pmcidPMC3134353
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907-2010, United States
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907-2010, United States
dc.contributor.institutionDivision of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, South Korea
dc.contributor.institutionDepartment of Agronomy and Medicinal Plant Resources, Gyeongnam National University of Science and Technology, Jinju 660-758, South Korea
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personBressan, Ray Anthony
kaust.personNarasimhan, Meena L.
refterms.dateFOA2018-06-14T04:31:50Z
dc.date.published-online2011-04-21
dc.date.published-print2011-07


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