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    YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana

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    Type
    Article
    Authors
    Kim, Jeong Im
    Murphy, Angus S.
    Baek, Dongwon
    Lee, Shin-Woo
    Yun, Dae-Jin
    Bressan, Ray Anthony
    Narasimhan, Meena L.
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Desert Agriculture Initiative
    Plant Stress Genomics Research Lab
    Date
    2011-04-21
    Online Publication Date
    2011-04-21
    Print Publication Date
    2011-07
    Permanent link to this record
    http://hdl.handle.net/10754/325445
    
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    Abstract
    The Arabidopsis thaliana YUCCA family of flavin monooxygenase proteins catalyses a rate-limiting step in de novo auxin biosynthesis. A YUCCA6 activation mutant, yuc6-1D, has been shown to contain an elevated free IAA level and to display typical high-auxin phenotypes. It is reported here that Arabidopsis plants over-expressing YUCCA6, such as the yuc6-1D activation mutant and 35S:YUC6 transgenic plants, displayed dramatic longevity. In addition, plants over-expressing YUCCA6 exhibited classical, delayed dark-induced and hormone-induced senescence in assays using detached rosette leaves. However, plants over-expressing an allele of YUCCA6, that carries mutations in the NADPH cofactor binding site, exhibited neither delayed leaf senescence phenotypes nor phenotypes typical of auxin overproduction. When the level of free IAA was reduced in yuc6-1D by conjugation to lysine, yuc6-1D leaves senesced at a rate similar to the wild-type leaves. Dark-induced senescence in detached leaves was accompanied by a decrease in their free IAA content, by the reduced expression of auxin biosynthesis enzymes such as YUCCA1 and YUCCA6 that increase cellular free IAA levels, and by the increased expression of auxin-conjugating enzymes encoded by the GH3 genes that reduce the cellular free auxin levels. Reduced transcript abundances of SAG12, NAC1, and NAC6 during senescence in yuc6-1D compared with the wild type suggested that auxin delays senescence by directly or indirectly regulating the expression of senescence-associated genes. 2011 The Author(s).
    Citation
    Kim JI, Murphy AS, Baek D, Lee S-W, Yun D-J, et al. (2011) YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana. Journal of Experimental Botany 62: 3981-3992. doi:10.1093/jxb/err094.
    Publisher
    Oxford University Press (OUP)
    Journal
    Journal of Experimental Botany
    DOI
    10.1093/jxb/err094
    PubMed ID
    21511905
    PubMed Central ID
    PMC3134353
    ae974a485f413a2113503eed53cd6c53
    10.1093/jxb/err094
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Desert Agriculture Initiative

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