YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionDesert Agriculture Initiative
Plant Stress Genomics Research Lab
Date
2011-04-21Online Publication Date
2011-04-21Print Publication Date
2011-07Permanent link to this record
http://hdl.handle.net/10754/325445Metadata
Show full item recordAbstract
The Arabidopsis thaliana YUCCA family of flavin monooxygenase proteins catalyses a rate-limiting step in de novo auxin biosynthesis. A YUCCA6 activation mutant, yuc6-1D, has been shown to contain an elevated free IAA level and to display typical high-auxin phenotypes. It is reported here that Arabidopsis plants over-expressing YUCCA6, such as the yuc6-1D activation mutant and 35S:YUC6 transgenic plants, displayed dramatic longevity. In addition, plants over-expressing YUCCA6 exhibited classical, delayed dark-induced and hormone-induced senescence in assays using detached rosette leaves. However, plants over-expressing an allele of YUCCA6, that carries mutations in the NADPH cofactor binding site, exhibited neither delayed leaf senescence phenotypes nor phenotypes typical of auxin overproduction. When the level of free IAA was reduced in yuc6-1D by conjugation to lysine, yuc6-1D leaves senesced at a rate similar to the wild-type leaves. Dark-induced senescence in detached leaves was accompanied by a decrease in their free IAA content, by the reduced expression of auxin biosynthesis enzymes such as YUCCA1 and YUCCA6 that increase cellular free IAA levels, and by the increased expression of auxin-conjugating enzymes encoded by the GH3 genes that reduce the cellular free auxin levels. Reduced transcript abundances of SAG12, NAC1, and NAC6 during senescence in yuc6-1D compared with the wild type suggested that auxin delays senescence by directly or indirectly regulating the expression of senescence-associated genes. 2011 The Author(s).Citation
Kim JI, Murphy AS, Baek D, Lee S-W, Yun D-J, et al. (2011) YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana. Journal of Experimental Botany 62: 3981-3992. doi:10.1093/jxb/err094.Publisher
Oxford University Press (OUP)Journal
Journal of Experimental BotanyPubMed ID
21511905PubMed Central ID
PMC3134353Scopus Count
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