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    Actinomycetes from red sea sponges: Sources for chemical and phylogenetic diversity

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    Article-Marine_Dru-Actinomyce-2014.pdf
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    Type
    Article
    Authors
    Abdelmohsen, Usama Ramadan
    Yang, Chen
    Horn, Hannes
    Hajjar, Dina A. cc
    Ravasi, Timothy cc
    Hentschel, Ute cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Chemical Engineering Program
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    Integrative Systems Biology Lab
    Date
    2014-05-12
    Permanent link to this record
    http://hdl.handle.net/10754/325360
    
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    Abstract
    The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery. 2014 by the authors; licensee MDPI.
    Citation
    Abdelmohsen U, Yang C, Horn H, Hajjar D, Ravasi T, et al. (2014) Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity. Marine Drugs 12: 2771-2789. doi:10.3390/md12052771.
    Publisher
    MDPI AG
    Journal
    Marine Drugs
    DOI
    10.3390/md12052771
    PubMed ID
    24824024
    PubMed Central ID
    PMC4052315
    ae974a485f413a2113503eed53cd6c53
    10.3390/md12052771
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Integrative Systems Biology Lab; Chemical Engineering Program; Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division

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