• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguidePlumX LibguideSubmit an Item

    Statistics

    Display statistics

    A scalable pipeline for highly effective genetic modification of a malaria parasite

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Article-Nature_Met-A_scalable-2011.pdf
    Size:
    1.327Mb
    Format:
    PDF
    Description:
    Article - Full Text
    Download
    Thumbnail
    Name:
    Supplement_1_-_Nature_Met-A_scalable-2011.NIHMS36792-supplement-1.pdf
    Size:
    528.7Kb
    Format:
    PDF
    Description:
    Supplemental File 1
    Download
    Type
    Article
    Authors
    Pfander, Claudia
    Anar, Burcu
    Schwach, Frank
    Otto, Thomas D.
    Brochet, Mathieu
    Volkmann, Katrin
    Quail, Michael A.
    Pain, Arnab cc
    Rosen, Barry
    Skarnes, William
    Rayner, Julian C.
    Billker, Oliver
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Computational Bioscience Research Center (CBRC)
    Pathogen Genomics Laboratory
    Date
    2011-10-23
    Online Publication Date
    2011-10-23
    Print Publication Date
    2011-12
    Permanent link to this record
    http://hdl.handle.net/10754/325358
    
    Metadata
    Show full item record
    Abstract
    In malaria parasites, the systematic experimental validation of drug and vaccine targets by reverse genetics is constrained by the inefficiency of homologous recombination and by the difficulty of manipulating adenine and thymine (A+T)-rich DNA of most Plasmodium species in Escherichia coli. We overcame these roadblocks by creating a high-integrity library of Plasmodium berghei genomic DNA (>77% A+T content) in a bacteriophage N15-based vector that can be modified efficiently using the lambda Red method of recombineering. We built a pipeline for generating P. berghei genetic modification vectors at genome scale in serial liquid cultures on 96-well plates. Vectors have long homology arms, which increase recombination frequency up to tenfold over conventional designs. The feasibility of efficient genetic modification at scale will stimulate collaborative, genome-wide knockout and tagging programs for P. berghei. © 2011 Nature America, Inc. All rights reserved.
    Citation
    Pfander C, Anar B, Schwach F, Otto TD, Brochet M, et al. (2011) A scalable pipeline for highly effective genetic modification of a malaria parasite. Nature Methods 8: 1078-1082. doi:10.1038/nmeth.1742.
    Publisher
    Springer Nature
    Journal
    Nature Methods
    DOI
    10.1038/nmeth.1742
    PubMed ID
    22020067
    PubMed Central ID
    PMC3431185
    ae974a485f413a2113503eed53cd6c53
    10.1038/nmeth.1742
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Computational Bioscience Research Center (CBRC)

    entitlement

    Related articles

    • Recombination-mediated genetic engineering of Plasmodium berghei DNA.
    • Authors: Pfander C, Anar B, Brochet M, Rayner JC, Billker O
    • Issue date: 2013
    • Expansion of experimental genetics approaches for Plasmodium berghei with versatile transfection vectors.
    • Authors: Kooij TW, Rauch MM, Matuschewski K
    • Issue date: 2012 Sep
    • PlasmoGEM, a database supporting a community resource for large-scale experimental genetics in malaria parasites.
    • Authors: Schwach F, Bushell E, Gomes AR, Anar B, Girling G, Herd C, Rayner JC, Billker O
    • Issue date: 2015 Jan
    • Multifunctional Involvement of a C2H2 Zinc Finger Protein (PbZfp) in Malaria Transmission, Histone Modification, and Susceptibility to DNA Damage Response.
    • Authors: Gopalakrishnan AM, Aly ASI, Aravind L, Kumar N
    • Issue date: 2017 Aug 29
    • Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites.
    • Authors: Fonager J, Franke-Fayard BM, Adams JH, Ramesar J, Klop O, Khan SM, Janse CJ, Waters AP
    • Issue date: 2011 Mar 20
    DSpace software copyright © 2002-2021  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.