Role of RNA interference (RNAi) in the moss Physcomitrella patens
KAUST DepartmentDesert Agriculture Initiative
Biological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/325355
MetadataShow full item record
AbstractRNA interference (RNAi) is a mechanism that regulates genes by either transcriptional (TGS) or posttranscriptional gene silencing (PTGS), required for genome maintenance and proper development of an organism. Small non-coding RNAs are the key players in RNAi and have been intensively studied in eukaryotes. In plants, several classes of small RNAs with specific sizes and dedicated functions have evolved. The major classes of small RNAs include microRNAs (miRNAs) and small interfering RNAs (siRNAs), which differ in their biogenesis. miRNAs are synthesized from a short hairpin structure while siRNAs are derived from long double-stranded RNAs (dsRNA). Both miRNA and siRNAs control the expression of cognate target RNAs by binding to reverse complementary sequences mediating cleavage or translational inhibition of the target RNA. They also act on the DNA and cause epigenetic changes such as DNA methylation and histone modifications. In the last years, the analysis of plant RNAi pathways was extended to the bryophyte Physcomitrella patens, a non-flowering, non-vascular ancient land plant that diverged from the lineage of seed plants approximately 450 million years ago. Based on a number of characteristic features and its phylogenetic key position in land plant evolution P. patens emerged as a plant model species to address basic as well as applied topics in plant biology. Here we summarize the current knowledge on the role of RNAi in P. patens that shows functional overlap with RNAi pathways from seed plants, and also unique features specific to this species. 2013 by the authors; licensee MDPI, Basel, Switzerland.
CitationArif M, Frank W, Khraiwesh B (2013) Role of RNA Interference (RNAi) in the Moss Physcomitrella patens. International Journal of Molecular Sciences 14: 1516-1540. doi:10.3390/ijms14011516.
PubMed Central IDPMC3565333
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
- Expression of artificial microRNAs in Physcomitrella patens.
- Authors: Fattash I, Khraiwesh B, Arif MA, Frank W
- Issue date: 2012
- Physcomitrella patens DCL3 is required for 22-24 nt siRNA accumulation, suppression of retrotransposon-derived transcripts, and normal development.
- Authors: Cho SH, Addo-Quaye C, Coruh C, Arif MA, Ma Z, Frank W, Axtell MJ
- Issue date: 2008 Dec
- MicroRNAs in the moss Physcomitrella patens.
- Authors: Arazi T
- Issue date: 2012 Sep
- Specific gene silencing by artificial MicroRNAs in Physcomitrella patens: an alternative to targeted gene knockouts.
- Authors: Khraiwesh B, Ossowski S, Weigel D, Reski R, Frank W
- Issue date: 2008 Oct
- Comprehensive Annotation of Physcomitrella patens Small RNA Loci Reveals That the Heterochromatic Short Interfering RNA Pathway Is Largely Conserved in Land Plants.
- Authors: Coruh C, Cho SH, Shahid S, Liu Q, Wierzbicki A, Axtell MJ
- Issue date: 2015 Aug