• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Comparative genomics of the apicomplexan parasites Toxoplasma gondii and neospora caninum: Coccidia differing in host range and transmission strategy

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Article-PLoS_Patho-Comparativ-2012.pdf
    Size:
    1.027Mb
    Format:
    PDF
    Description:
    Article - Full Text
    Download
    Thumbnail
    Name:
    Supplement_1_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s001.eps
    Size:
    3.420Mb
    Format:
    Postscript
    Description:
    Supplemental File 1
    Download
    Thumbnail
    Name:
    Supplement_2_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s002.eps
    Size:
    9.568Mb
    Format:
    Postscript
    Description:
    Supplemental File 2
    Download
    Thumbnail
    Name:
    Supplement_3_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s003.eps
    Size:
    993.6Kb
    Format:
    Postscript
    Description:
    Supplemental File 3
    Download
    Thumbnail
    Name:
    Supplement_4_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s004.eps
    Size:
    951.7Kb
    Format:
    Postscript
    Description:
    Supplemental File 4
    Download
    Thumbnail
    Name:
    Supplement_5_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s005.eps
    Size:
    359.2Kb
    Format:
    Postscript
    Description:
    Supplemental File 5
    Download
    Thumbnail
    Name:
    Supplement_6_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s006.eps
    Size:
    987.5Kb
    Format:
    Postscript
    Description:
    Supplemental File 6
    Download
    Thumbnail
    Name:
    Supplement_7_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s007.eps
    Size:
    475.7Kb
    Format:
    Postscript
    Description:
    Supplemental File 7
    Download
    Thumbnail
    Name:
    Supplement_8_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s008.eps
    Size:
    712.1Kb
    Format:
    Postscript
    Description:
    Supplemental File 8
    Download
    Thumbnail
    Name:
    Supplement_9_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s009.eps
    Size:
    1.212Mb
    Format:
    Postscript
    Description:
    Supplemental File 9
    Download
    Thumbnail
    Name:
    Supplement_10_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s010.docx
    Size:
    104.0Kb
    Format:
    Microsoft Word 2007
    Description:
    Supplemental File 10
    Download
    Thumbnail
    Name:
    Supplement_11_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s011.docx
    Size:
    66.04Kb
    Format:
    Microsoft Word 2007
    Description:
    Supplemental File 11
    Download
    Thumbnail
    Name:
    Supplement_12_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s012.docx
    Size:
    42.17Kb
    Format:
    Microsoft Word 2007
    Description:
    Supplemental File 12
    Download
    Thumbnail
    Name:
    Supplement_13_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s013.docx
    Size:
    114.9Kb
    Format:
    Microsoft Word 2007
    Description:
    Supplemental File 13
    Download
    Thumbnail
    Name:
    Supplement_14_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s014.docx
    Size:
    85.89Kb
    Format:
    Microsoft Word 2007
    Description:
    Supplemental File 14
    Download
    Thumbnail
    Name:
    Supplement_15_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s015.docx
    Size:
    55.02Kb
    Format:
    Microsoft Word 2007
    Description:
    Supplemental File 15
    Download
    Thumbnail
    Name:
    Supplement_16_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s016.docx
    Size:
    98.51Kb
    Format:
    Microsoft Word 2007
    Description:
    Supplemental File 16
    Download
    Thumbnail
    Name:
    Supplement_17_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s017.doc
    Size:
    39Kb
    Format:
    Microsoft Word
    Description:
    Supplemental File 17
    Download
    Thumbnail
    Name:
    Supplement_18_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s018.doc
    Size:
    93.5Kb
    Format:
    Microsoft Word
    Description:
    Supplemental File 18
    Download
    Thumbnail
    Name:
    Supplement_19_-_PLoS_Patho-Comparativ-2012.ppat.1002567.s019.docx
    Size:
    107.7Kb
    Format:
    Microsoft Word 2007
    Description:
    Supplemental File 19
    Download
    View more filesView fewer files
    Type
    Article
    Authors
    Reid, Adam James
    Vermont, Sarah J.
    Cotton, James A.
    Harris, David
    Hill-Cawthorne, Grant A. cc
    Könen-Waisman, Stephanie
    Latham, Sophia M.
    Mourier, Tobias
    Norton, Rebecca
    Quail, Michael A.
    Sanders, Mandy
    Shanmugam, Dhanasekaran cc
    Sohal, Amandeep
    Wasmuth, James D.
    Brunk, Brian
    Grigg, Michael E.
    Howard, Jonathan C.
    Parkinson, John
    Roos, David S.
    Trees, Alexander J.
    Berriman, Matthew
    Pain, Arnab cc
    Wastling, Jonathan M.
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Computational Bioscience Research Center (CBRC)
    Pathogen Genomics Laboratory
    Date
    2012-03-22
    Permanent link to this record
    http://hdl.handle.net/10754/325344
    
    Metadata
    Show full item record
    Abstract
    Toxoplasma gondii is a zoonotic protozoan parasite which infects nearly one third of the human population and is found in an extraordinary range of vertebrate hosts. Its epidemiology depends heavily on horizontal transmission, especially between rodents and its definitive host, the cat. Neospora caninum is a recently discovered close relative of Toxoplasma, whose definitive host is the dog. Both species are tissue-dwelling Coccidia and members of the phylum Apicomplexa; they share many common features, but Neospora neither infects humans nor shares the same wide host range as Toxoplasma, rather it shows a striking preference for highly efficient vertical transmission in cattle. These species therefore provide a remarkable opportunity to investigate mechanisms of host restriction, transmission strategies, virulence and zoonotic potential. We sequenced the genome of N. caninum and transcriptomes of the invasive stage of both species, undertaking an extensive comparative genomics and transcriptomics analysis. We estimate that these organisms diverged from their common ancestor around 28 million years ago and find that both genomes and gene expression are remarkably conserved. However, in N. caninum we identified an unexpected expansion of surface antigen gene families and the divergence of secreted virulence factors, including rhoptry kinases. Specifically we show that the rhoptry kinase ROP18 is pseudogenised in N. caninum and that, as a possible consequence, Neospora is unable to phosphorylate host immunity-related GTPases, as Toxoplasma does. This defense strategy is thought to be key to virulence in Toxoplasma. We conclude that the ecological niches occupied by these species are influenced by a relatively small number of gene products which operate at the host-parasite interface and that the dominance of vertical transmission in N. caninum may be associated with the evolution of reduced virulence in this species.
    Citation
    Reid AJ, Vermont SJ, Cotton JA, Harris D, Hill-Cawthorne GA, et al. (2012) Comparative Genomics of the Apicomplexan Parasites Toxoplasma gondii and Neospora caninum: Coccidia Differing in Host Range and Transmission Strategy. PLoS Pathog 8: e1002567. doi:10.1371/journal.ppat.1002567.
    Publisher
    Public Library of Science (PLoS)
    Journal
    PLoS Pathogens
    DOI
    10.1371/journal.ppat.1002567
    PubMed ID
    22457617
    PubMed Central ID
    PMC3310773
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.ppat.1002567
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Computational Bioscience Research Center (CBRC)

    entitlement

    Related articles

    • Toxoplasma gondii and Neospora caninum induce different host cell responses at proteome-wide phosphorylation events; a step forward for uncovering the biological differences between these closely related parasites.
    • Authors: Al-Bajalan MMM, Xia D, Armstrong S, Randle N, Wastling JM
    • Issue date: 2017 Oct
    • ROP18 is a key factor responsible for virulence difference between Toxoplasma gondii and Neospora caninum.
    • Authors: Lei T, Wang H, Liu J, Nan H, Liu Q
    • Issue date: 2014
    • Comprehensive evaluation of Toxoplasma gondii VEG and Neospora caninum LIV genomes with tachyzoite stage transcriptome and proteome defines novel transcript features.
    • Authors: Ramaprasad A, Mourier T, Naeem R, Malas TB, Moussa E, Panigrahi A, Vermont SJ, Otto TD, Wastling J, Pain A
    • Issue date: 2015
    • Secreted effectors in Toxoplasma gondii and related species: determinants of host range and pathogenesis?
    • Authors: English ED, Adomako-Ankomah Y, Boyle JP
    • Issue date: 2015 Mar
    • Comparison of the major antigens of Neospora caninum and Toxoplasma gondii.
    • Authors: Howe DK, Sibley LD
    • Issue date: 1999 Oct

    Related items

    Showing items related by title, author, creator and subject.

    • Thumbnail

      This is a genome assembly third party annotation of Toxoplasma gondii VEG strain based on strand-specific RNA-sequencing and manual re-annotation identifying novel features of UTRs and non-coding transcripts.

      Ramaprasad, Abhinay; Mourier, Tobias; Naeem, Raeece; Moussa, Ehab; Vermont, Sarah J.; Otto, Thomas D.; Wastling, Jonathan; Pain, Arnab; Malas, Tareq Majed Yasin; Panigrahi, Aswini Kumar (NCBI, 2015-02-26) [Bioproject, Dataset]
      Toxoplasma gondii is an important protozoan parasite that infects all warm-blooded animals and causes opportunistic infections in immuno-compromised humans. Its closest relative, Neospora caninum, is an important veterinary pathogen that causes spontaneous abortion in livestock. Comparative genomics of these two closely related coccidians has been of particular interest to identify genes that contribute to varied host cell specificity and disease. Automated gene prediction tools that were used for gene annotation can lead to inaccurate gene models and lack information on untranslated regions and non-coding transcripts. Here, we describe a manual re-annotation of these genomes based on strand-specific RNA sequencing and shotgun proteomics. We have corrected the structures of over one third of the gene models and have annotated the complete set of untranslated regions (UTRs). We observe distinctly long UTRs in both the ?organisms??, almost four times longer than other eukaryotes?. We have also identified a putative set of cis-natural antisense transcripts (cis-NATs) and long intergenic non-coding RNAs (lincRNAs). With these, we have significantly improved the quality of annotation in the genomes to serve as a manually curated base for future research on these organisms.
    • Thumbnail

      Comprehensive Evaluation of Toxoplasma gondii VEG and Neospora caninum LIV Genomes with Tachyzoite Stage Transcriptome and Proteome Defines Novel Transcript Features

      Ramaprasad, Abhinay; Mourier, Tobias; Naeem, Raeece; Malas, Tareq Majed Yasin; Moussa, Ehab; Panigrahi, Aswini Kumar; Vermont, Sarah J.; Otto, Thomas D.; Wastling, Jonathan; Pain, Arnab (PLoS ONE, Public Library of Science (PLoS), 2015-04-13) [Article]
      Toxoplasma gondii is an important protozoan parasite that infects all warm-blooded animals and causes opportunistic infections in immuno-compromised humans. Its closest relative, Neospora caninum, is an important veterinary pathogen that causes spontaneous abortion in livestock. Comparative genomics of these two closely related coccidians has been of particular interest to identify genes that contribute to varied host cell specificity and disease. Here, we describe a manual evaluation of these genomes based on strand-specific RNA sequencing and shotgun proteomics from the invasive tachyzoite stages of these two parasites. We have corrected predicted structures of over one third of the previously annotated gene models and have annotated untranslated regions (UTRs) in over half of the predicted protein-coding genes. We observe distinctly long UTRs in both the organisms, almost four times longer than other model eukaryotes. We have also identified a putative set of cis-natural antisense transcripts (cis-NATs) and long intergenic non-coding RNAs (lincRNAs). We have significantly improved the annotation quality in these genomes that would serve as a manually curated dataset for Toxoplasma and Neospora research communities.
    • Thumbnail

      Development and Application of Classical Genetics in Toxoplasma gondii

      Ajioka, James W.; Sibley, L. David (Elsevier, 2014) [Book Chapter]
      Toxoplasma gondii undergoes its sexual phase within the feline intestine. The sexual stages have been an important tool for understanding the biology of the parasite using forward genetics approaches. Genetic crosses were performed by feeding cats bradyzoites from T. gondii strains that differ in virulence and drug resistance. Using progeny from these crosses, the genes responsible for virulence and other biologically important phenotypes have been mapped using classical forward genetic techniques. This chapter reviews the principles of forward genetics in T. gondii and illustrates how the classical genetics approach enabled identification of Toxoplasma virulence genes including the ROP family of rhoptry kinases and pseudokinases. © 2014 Elsevier Ltd. All rights reserved.
    DSpace software copyright © 2002-2023  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.