Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio
dc.contributor.author | Gomes-Santos, Carina S. S. | * |
dc.contributor.author | Braks, Joanna | * |
dc.contributor.author | Prudêncio, Miguel | * |
dc.contributor.author | Carret, Céline | * |
dc.contributor.author | Gomes, Ana Rita | * |
dc.contributor.author | Pain, Arnab | * |
dc.contributor.author | Feltwell, Theresa | * |
dc.contributor.author | Khan, Shahid | * |
dc.contributor.author | Waters, Andrew | * |
dc.contributor.author | Janse, Chris | * |
dc.contributor.author | Mair, Gunnar R. | * |
dc.contributor.author | Mota, Maria M. | * |
dc.date.accessioned | 2014-08-27T09:48:13Z | |
dc.date.available | 2014-08-27T09:48:13Z | |
dc.date.issued | 2011-05-19 | en |
dc.identifier.citation | Gomes-Santos CSS, Braks J, Prudêncio M, Carret C, Gomes AR, et al. (2011) Transition of Plasmodium Sporozoites into Liver Stage-Like Forms Is Regulated by the RNA Binding Protein Pumilio. PLoS Pathog 7: e1002046. doi:10.1371/journal.ppat.1002046. | en |
dc.identifier.issn | 15537366 | en |
dc.identifier.pmid | 21625527 | en |
dc.identifier.doi | 10.1371/journal.ppat.1002046 | en |
dc.identifier.uri | http://hdl.handle.net/10754/325343 | en |
dc.description.abstract | Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host. Here we identify a Plasmodium member of the RNA binding protein family PUF as a key regulator of this transformation. In the absence of Pumilio-2 (Puf2) sporozoites initiate EEF development inside mosquito salivary glands independently of the normal transmission-associated environmental cues. Puf2- sporozoites exhibit genome-wide transcriptional changes that result in loss of gliding motility, cell traversal ability and reduction in infectivity, and, moreover, trigger metamorphosis typical of early Plasmodium intra-hepatic development. These data demonstrate that Puf2 is a key player in regulating sporozoite developmental control, and imply that transformation of salivary gland-resident sporozoites into liver stage-like parasites is regulated by a post-transcriptional mechanism. 2011 Gomes-Santos et al. | en |
dc.language.iso | en | en |
dc.publisher | Public Library of Science (PLoS) | en |
dc.rights | Gomes-Santos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.rights | Archived with thanks to PLoS Pathogens | en |
dc.subject | RNA binding protein | en |
dc.subject | RNA binding protein pumilio | en |
dc.subject | transcriptome | en |
dc.subject | unclassified drug | en |
dc.subject | protozoal protein | en |
dc.subject | RNA binding protein | en |
dc.subject | asexual reproduction | en |
dc.subject | controlled study | en |
dc.subject | disease transmission | en |
dc.subject | environment | en |
dc.subject | gene expression | en |
dc.subject | genetic analysis | en |
dc.subject | metamorphosis | en |
dc.subject | mosquito | en |
dc.subject | Northern blotting | en |
dc.subject | Plasmodium | en |
dc.subject | posttranscriptional gene silencing | en |
dc.subject | reverse transcription polymerase chain reaction | en |
dc.subject | salivary gland | en |
dc.subject | sporozoite | en |
dc.subject | Anopheles | en |
dc.subject | Bagg albino mouse | en |
dc.subject | C57BL mouse | en |
dc.subject | gene expression profiling | en |
dc.subject | genetics | en |
dc.subject | growth, development and aging | en |
dc.subject | liver | en |
dc.subject | malaria | en |
dc.subject | metabolism | en |
dc.subject | microarray analysis | en |
dc.subject | mouse | en |
dc.subject | parasitology | en |
dc.subject | pathogenicity | en |
dc.subject | Plasmodium berghei | en |
dc.subject | protein processing | en |
dc.subject | ultrastructure | en |
dc.subject | Eukaryota | en |
dc.subject | Plasmodium (Apicomplexa) | en |
dc.subject | Vertebrata | en |
dc.subject | Anopheles | en |
dc.subject | Gene Expression Profiling | en |
dc.subject | Liver | en |
dc.subject | Malaria | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred BALB C | en |
dc.subject | Mice, Inbred C57BL | en |
dc.subject | Microarray Analysis | en |
dc.subject | Plasmodium berghei | en |
dc.subject | Protein Processing, Post-Translational | en |
dc.subject | Protozoan Proteins | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | RNA-Binding Proteins | en |
dc.subject | Salivary Glands | en |
dc.subject | Sporozoites | en |
dc.title | Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio | en |
dc.type | Article | en |
dc.contributor.department | Biological and Environmental Sciences and Engineering (BESE) Division | * |
dc.contributor.department | Computational Bioscience Research Center (CBRC) | * |
dc.identifier.journal | PLoS Pathogens | en |
dc.identifier.pmcid | PMC3098293 | en |
dc.eprint.version | Publisher's Version/PDF | en |
dc.contributor.institution | Malaria Unit, Instituto de Medicina Molecular, Lisboa, Portugal | * |
dc.contributor.institution | PhD Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal | * |
dc.contributor.institution | Leiden Malaria Research Group, Parasitology, Leiden University Medical Centre, Leiden, Netherlands | * |
dc.contributor.institution | Molecular Parasitology Unit, Instituto de Medicina Molecular, Lisbon, Portugal | * |
dc.contributor.institution | Pathogen Genetics Group, Wellcome Trust Sanger Institute, Cambridge, United Kingdom | * |
dc.contributor.institution | Division of Infection and Immunity, Institute of Biomedical Life Sciences and Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, United Kingdom | * |
dc.contributor.affiliation | King Abdullah University of Science and Technology (KAUST) | * |
kaust.author | Pain, Arnab | * |
refterms.dateFOA | 2018-06-13T15:15:21Z |