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dc.contributor.authorGomes-Santos, Carina S. S.*
dc.contributor.authorBraks, Joanna*
dc.contributor.authorPrudêncio, Miguel*
dc.contributor.authorCarret, Céline*
dc.contributor.authorGomes, Ana Rita*
dc.contributor.authorPain, Arnab*
dc.contributor.authorFeltwell, Theresa*
dc.contributor.authorKhan, Shahid*
dc.contributor.authorWaters, Andrew*
dc.contributor.authorJanse, Chris*
dc.contributor.authorMair, Gunnar R.*
dc.contributor.authorMota, Maria M.*
dc.date.accessioned2014-08-27T09:48:13Z
dc.date.available2014-08-27T09:48:13Z
dc.date.issued2011-05-19en
dc.identifier.citationGomes-Santos CSS, Braks J, Prudêncio M, Carret C, Gomes AR, et al. (2011) Transition of Plasmodium Sporozoites into Liver Stage-Like Forms Is Regulated by the RNA Binding Protein Pumilio. PLoS Pathog 7: e1002046. doi:10.1371/journal.ppat.1002046.en
dc.identifier.issn15537366en
dc.identifier.pmid21625527en
dc.identifier.doi10.1371/journal.ppat.1002046en
dc.identifier.urihttp://hdl.handle.net/10754/325343en
dc.description.abstractMany eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host. Here we identify a Plasmodium member of the RNA binding protein family PUF as a key regulator of this transformation. In the absence of Pumilio-2 (Puf2) sporozoites initiate EEF development inside mosquito salivary glands independently of the normal transmission-associated environmental cues. Puf2- sporozoites exhibit genome-wide transcriptional changes that result in loss of gliding motility, cell traversal ability and reduction in infectivity, and, moreover, trigger metamorphosis typical of early Plasmodium intra-hepatic development. These data demonstrate that Puf2 is a key player in regulating sporozoite developmental control, and imply that transformation of salivary gland-resident sporozoites into liver stage-like parasites is regulated by a post-transcriptional mechanism. 2011 Gomes-Santos et al.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.rightsGomes-Santos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rightsArchived with thanks to PLoS Pathogensen
dc.subjectRNA binding proteinen
dc.subjectRNA binding protein pumilioen
dc.subjecttranscriptomeen
dc.subjectunclassified drugen
dc.subjectprotozoal proteinen
dc.subjectRNA binding proteinen
dc.subjectasexual reproductionen
dc.subjectcontrolled studyen
dc.subjectdisease transmissionen
dc.subjectenvironmenten
dc.subjectgene expressionen
dc.subjectgenetic analysisen
dc.subjectmetamorphosisen
dc.subjectmosquitoen
dc.subjectNorthern blottingen
dc.subjectPlasmodiumen
dc.subjectposttranscriptional gene silencingen
dc.subjectreverse transcription polymerase chain reactionen
dc.subjectsalivary glanden
dc.subjectsporozoiteen
dc.subjectAnophelesen
dc.subjectBagg albino mouseen
dc.subjectC57BL mouseen
dc.subjectgene expression profilingen
dc.subjectgeneticsen
dc.subjectgrowth, development and agingen
dc.subjectliveren
dc.subjectmalariaen
dc.subjectmetabolismen
dc.subjectmicroarray analysisen
dc.subjectmouseen
dc.subjectparasitologyen
dc.subjectpathogenicityen
dc.subjectPlasmodium bergheien
dc.subjectprotein processingen
dc.subjectultrastructureen
dc.subjectEukaryotaen
dc.subjectPlasmodium (Apicomplexa)en
dc.subjectVertebrataen
dc.subjectAnophelesen
dc.subjectGene Expression Profilingen
dc.subjectLiveren
dc.subjectMalariaen
dc.subjectMiceen
dc.subjectMice, Inbred BALB Cen
dc.subjectMice, Inbred C57BLen
dc.subjectMicroarray Analysisen
dc.subjectPlasmodium bergheien
dc.subjectProtein Processing, Post-Translationalen
dc.subjectProtozoan Proteinsen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectRNA-Binding Proteinsen
dc.subjectSalivary Glandsen
dc.subjectSporozoitesen
dc.titleTransition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilioen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division*
dc.contributor.departmentComputational Bioscience Research Center (CBRC)*
dc.identifier.journalPLoS Pathogensen
dc.identifier.pmcidPMC3098293en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionMalaria Unit, Instituto de Medicina Molecular, Lisboa, Portugal*
dc.contributor.institutionPhD Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal*
dc.contributor.institutionLeiden Malaria Research Group, Parasitology, Leiden University Medical Centre, Leiden, Netherlands*
dc.contributor.institutionMolecular Parasitology Unit, Instituto de Medicina Molecular, Lisbon, Portugal*
dc.contributor.institutionPathogen Genetics Group, Wellcome Trust Sanger Institute, Cambridge, United Kingdom*
dc.contributor.institutionDivision of Infection and Immunity, Institute of Biomedical Life Sciences and Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, United Kingdom*
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)*
kaust.authorPain, Arnab*
refterms.dateFOA2018-06-13T15:15:21Z


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