Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio
Name:
Article-PLoS_Patho-Transition-2011.pdf
Size:
1.093Mb
Format:
PDF
Description:
Article - Full Text
Name:
Supplement_1_-_PLoS_Patho-Transition-2011.ppat.1002046.s001.tif
Size:
326.5Kb
Format:
TIFF image
Description:
Supplemental File 1
Name:
Supplement_2_-_PLoS_Patho-Transition-2011.ppat.1002046.s002.tif
Size:
197.9Kb
Format:
TIFF image
Description:
Supplemental File 2
Name:
Supplement_3_-_PLoS_Patho-Transition-2011.ppat.1002046.s003.tif
Size:
251.0Kb
Format:
TIFF image
Description:
Supplemental File 3
Name:
Supplement_4_-_PLoS_Patho-Transition-2011.ppat.1002046.s004.tif
Size:
81.19Kb
Format:
TIFF image
Description:
Supplemental File 4
Name:
Supplement_5_-_PLoS_Patho-Transition-2011.ppat.1002046.s005.tif
Size:
81.79Kb
Format:
TIFF image
Description:
Supplemental File 5
Name:
Supplement_6_-_PLoS_Patho-Transition-2011.ppat.1002046.s006.tif
Size:
358.8Kb
Format:
TIFF image
Description:
Supplemental File 6
Name:
Supplement_7_-_PLoS_Patho-Transition-2011.ppat.1002046.s007.tif
Size:
215.0Kb
Format:
TIFF image
Description:
Supplemental File 7
Name:
Supplement_8_-_PLoS_Patho-Transition-2011.ppat.1002046.s008.tif
Size:
196.0Kb
Format:
TIFF image
Description:
Supplemental File 8
Name:
Supplement_9_-_PLoS_Patho-Transition-2011.ppat.1002046.s009.tif
Size:
264.0Kb
Format:
TIFF image
Description:
Supplemental File 9
Name:
Supplement_10_-_PLoS_Patho-Transition-2011.ppat.1002046.s010.jpg
Size:
213.1Kb
Format:
JPEG image
Description:
Supplemental File 10
Name:
Supplement_11_-_PLoS_Patho-Transition-2011.ppat.1002046.s011.doc
Size:
36Kb
Format:
Microsoft Word
Description:
Supplemental File 11
Name:
Supplement_12_-_PLoS_Patho-Transition-2011.ppat.1002046.s012.doc
Size:
31Kb
Format:
Microsoft Word
Description:
Supplemental File 12
Name:
Supplement_13_-_PLoS_Patho-Transition-2011.ppat.1002046.s013.xls
Size:
334Kb
Format:
Microsoft Excel
Description:
Supplemental File 13
Name:
Supplement_14_-_PLoS_Patho-Transition-2011.ppat.1002046.s014.doc
Size:
29.5Kb
Format:
Microsoft Word
Description:
Supplemental File 14
Name:
Supplement_15_-_PLoS_Patho-Transition-2011.ppat.1002046.s015.doc
Size:
37Kb
Format:
Microsoft Word
Description:
Supplemental File 15
Name:
Supplement_16_-_PLoS_Patho-Transition-2011.ppat.1002046.s016.doc
Size:
29.5Kb
Format:
Microsoft Word
Description:
Supplemental File 16
Name:
Supplement_17_-_PLoS_Patho-Transition-2011.ppat.1002046.s017.doc
Size:
43Kb
Format:
Microsoft Word
Description:
Supplemental File 17
Name:
Supplement_18_-_PLoS_Patho-Transition-2011.ppat.1002046.s018.doc
Size:
36.5Kb
Format:
Microsoft Word
Description:
Supplemental File 18
Name:
Supplement_19_-_PLoS_Patho-Transition-2011.ppat.1002046.s019.avi
Size:
2.858Mb
Format:
Unknown
Description:
Supplemental File 19
Name:
Supplement_20_-_PLoS_Patho-Transition-2011.ppat.1002046.s020.avi
Size:
2.945Mb
Format:
Unknown
Description:
Supplemental File 20
Name:
Supplement_21_-_PLoS_Patho-Transition-2011.ppat.1002046.s021.avi
Size:
3.005Mb
Format:
Unknown
Description:
Supplemental File 21
Type
ArticleAuthors
Gomes-Santos, Carina S. S.Braks, Joanna
Prudêncio, Miguel
Carret, Céline
Gomes, Ana Rita
Pain, Arnab

Feltwell, Theresa
Khan, Shahid
Waters, Andrew
Janse, Chris
Mair, Gunnar R.
Mota, Maria M.
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Computational Bioscience Research Center (CBRC)
Pathogen Genomics Laboratory
Date
2011-05-19Permanent link to this record
http://hdl.handle.net/10754/325343
Metadata
Show full item recordAbstract
Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host. Here we identify a Plasmodium member of the RNA binding protein family PUF as a key regulator of this transformation. In the absence of Pumilio-2 (Puf2) sporozoites initiate EEF development inside mosquito salivary glands independently of the normal transmission-associated environmental cues. Puf2- sporozoites exhibit genome-wide transcriptional changes that result in loss of gliding motility, cell traversal ability and reduction in infectivity, and, moreover, trigger metamorphosis typical of early Plasmodium intra-hepatic development. These data demonstrate that Puf2 is a key player in regulating sporozoite developmental control, and imply that transformation of salivary gland-resident sporozoites into liver stage-like parasites is regulated by a post-transcriptional mechanism. 2011 Gomes-Santos et al.Citation
Gomes-Santos CSS, Braks J, Prudêncio M, Carret C, Gomes AR, et al. (2011) Transition of Plasmodium Sporozoites into Liver Stage-Like Forms Is Regulated by the RNA Binding Protein Pumilio. PLoS Pathog 7: e1002046. doi:10.1371/journal.ppat.1002046.Publisher
Public Library of Science (PLoS)Journal
PLoS PathogensPubMed ID
21625527PubMed Central ID
PMC3098293ae974a485f413a2113503eed53cd6c53
10.1371/journal.ppat.1002046
Scopus Count
Related articles
- The Puf-family RNA-binding protein Puf2 controls sporozoite conversion to liver stages in the malaria parasite.
- Authors: Müller K, Matuschewski K, Silvie O
- Issue date: 2011
- Translational Control of UIS4 Protein of the Host-Parasite Interface Is Mediated by the RNA Binding Protein Puf2 in Plasmodium berghei Sporozoites.
- Authors: Silva PA, Guerreiro A, Santos JM, Braks JA, Janse CJ, Mair GR
- Issue date: 2016
- A Novel and Conserved Plasmodium Sporozoite Membrane Protein SPELD is Required for Maturation of Exo-erythrocytic Forms.
- Authors: Al-Nihmi FM, Kolli SK, Reddy SR, Mastan BS, Togiri J, Maruthi M, Gupta R, Sijwali PS, Mishra S, Kumar KA
- Issue date: 2017 Jan 9
- Disruption of Plasmodium sporozoite transmission by depletion of sporozoite invasion-associated protein 1.
- Authors: Engelmann S, Silvie O, Matuschewski K
- Issue date: 2009 Apr
- Plasmodium berghei sporozoite specific genes- PbS10 and PbS23/SSP3 are required for the development of exo-erythrocytic forms.
- Authors: Togiri J, Segireddy RR, Mastan BS, Singh D, Kolli SK, Ghosh A, Al-Nihmi FMA, Maruthi M, Choudhary HH, Dey S, Mishra S, Kumar KA
- Issue date: 2019 Sep
Related items
Showing items related by title, author, creator and subject.
-
Defining the protein interaction network of human malaria parasite Plasmodium falciparumRamaprasad, Abhinay; Pain, Arnab; Ravasi, Timothy (Genomics, Elsevier BV, 2012-02) [Article]Malaria, caused by the protozoan parasite Plasmodium falciparum, affects around 225. million people yearly and a huge international effort is directed towards combating this grave threat to world health and economic development. Considerable advances have been made in malaria research triggered by the sequencing of its genome in 2002, followed by several high-throughput studies defining the malaria transcriptome and proteome. A protein-protein interaction (PPI) network seeks to trace the dynamic interactions between proteins, thereby elucidating their local and global functional relationships. Experimentally derived PPI network from high-throughput methods such as yeast two hybrid (Y2H) screens are inherently noisy, but combining these independent datasets by computational methods tends to give a greater accuracy and coverage. This review aims to discuss the computational approaches used till date to construct a malaria protein interaction network and to catalog the functional predictions and biological inferences made from analysis of the PPI network. © 2011 Elsevier Inc.
-
Normocyte-binding protein required for human erythrocyte invasion by the zoonotic malaria parasitePlasmodium knowlesiMoon, Robert W.; Sharaf, Hazem; Hastings, Claire H.; Shwen, Ho; Nair, Mridul; Rchiad, Zineb; Knuepfer, Ellen; Ramaprasad, Abhinay; Mohring, Franziska; Amir, Amirah; Yusuf, Noor A.; Hall, Joanna; Almond, Neil; Lau, Yee Ling; Pain, Arnab; Blackman, Michael J.; Holder, Anthony A. (Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, 2016-06-14) [Article]The dominant cause of malaria in Malaysia is now Plasmodium knowlesi, a zoonotic parasite of cynomolgus macaque monkeys found throughout South East Asia. Comparative genomic analysis of parasites adapted to in vitro growth in either cynomolgus or human RBCs identified a genomic deletion that includes the gene encoding normocyte-binding protein Xa (NBPXa) in parasites growing in cynomolgus RBCs but not in human RBCs. Experimental deletion of the NBPXa gene in parasites adapted to growth in human RBCs (which retain the ability to grow in cynomolgus RBCs) restricted them to cynomolgus RBCs, demonstrating that this gene is selectively required for parasite multiplication and growth in human RBCs. NBPXa-null parasites could bind to human RBCs, but invasion of these cells was severely impaired. Therefore, NBPXa is identified as a key mediator of P. knowlesi human infection and may be a target for vaccine development against this emerging pathogen.
-
Targeted disruption of py235ebp-1: Invasion of erythrocytes by Plasmodium yoelii using an alternative py235 erythrocyte binding proteinOgun, Solabomi A.; Tewari, Rita; Otto, Thomas D.; Howell, Steven A.; Knuepfer, Ellen; Cunningham, Deirdre A.; Xu, Zhengyao; Pain, Arnab; Holder, Anthony A. (PLoS Pathogens, Public Library of Science (PLoS), 2011-02-17) [Article]Plasmodium yoelii YM asexual blood stage parasites express multiple members of the py235 gene family, part of the super-family of genes including those coding for Plasmodium vivax reticulocyte binding proteins and Plasmodium falciparum RH proteins. We previously identified a Py235 erythrocyte binding protein (Py235EBP-1, encoded by the PY01365 gene) that is recognized by protective mAb 25.77. Proteins recognized by a second protective mAb 25.37 have been identified by mass spectrometry and are encoded by two genes, PY01185 and PY05995/PY03534. We deleted the PY01365 gene and examined the phenotype. The expression of the members of the py235 family in both the WT and gene deletion parasites was measured by quantitative RT-PCR and RNA-Seq. py235ebp-1 expression was undetectable in the knockout parasite, but transcription of other members of the family was essentially unaffected. The knockout parasites continued to react with mAb 25.77; and the 25.77-binding proteins in these parasites were the PY01185 and PY05995/PY03534 products. The PY01185 product was also identified as erythrocyte binding. There was no clear change in erythrocyte invasion profile suggesting that the PY01185 gene product (designated PY235EBP-2) is able to fulfill the role of EBP-1 by serving as an invasion ligand although the molecular details of its interaction with erythrocytes have not been examined. The PY01365, PY01185, and PY05995/PY03534 genes are part of a distinct subset of the py235 family. In P. falciparum, the RH protein genes are under epigenetic control and expression correlates with binding to distinct erythrocyte receptors and specific invasion pathways, whereas in P. yoelii YM all the genes are expressed and deletion of one does not result in upregulation of another. We propose that simultaneous expression of multiple Py235 ligands enables invasion of a wide range of host erythrocytes even in the presence of antibodies to one or more of the proteins and that this functional redundancy at the protein level gives the parasite phenotypic plasticity in the absence of differences in gene expression. © 2011 Ogun et al.