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dc.contributor.authorHonsdorf, Nora
dc.contributor.authorMarch, Timothy John
dc.contributor.authorBerger, Bettina
dc.contributor.authorTester, Mark A.
dc.contributor.authorPillen, Klaus
dc.date.accessioned2014-08-27T09:47:47Z
dc.date.available2014-08-27T09:47:47Z
dc.date.issued2014-05-13
dc.identifier.citationHonsdorf N, March TJ, Berger B, Tester M, Pillen K (2014) High-Throughput Phenotyping to Detect Drought Tolerance QTL in Wild Barley Introgression Lines. PLoS ONE 9: e97047. doi:10.1371/journal.pone.0097047.
dc.identifier.issn19326203
dc.identifier.pmid24823485
dc.identifier.doi10.1371/journal.pone.0097047
dc.identifier.urihttp://hdl.handle.net/10754/325338
dc.description.abstractDrought is one of the most severe stresses, endangering crop yields worldwide. In order to select drought tolerant genotypes, access to exotic germplasm and efficient phenotyping protocols are needed. In this study the high-throughput phenotyping platform "The Plant Accelerator", Adelaide, Australia, was used to screen a set of 47 juvenile (six week old) wild barley introgression lines (S42ILs) for drought stress responses. The kinetics of growth development was evaluated under early drought stress and well watered treatments. High correlation (r = 0.98) between image based biomass estimates and actual biomass was demonstrated, and the suitability of the system to accurately and non-destructively estimate biomass was validated. Subsequently, quantitative trait loci (QTL) were located, which contributed to the genetic control of growth under drought stress. In total, 44 QTL for eleven out of 14 investigated traits were mapped, which for example controlled growth rate and water use efficiency. The correspondence of those QTL with QTL previously identified in field trials is shown. For instance, six out of eight QTL controlling plant height were also found in previous field and glasshouse studies with the same introgression lines. This indicates that phenotyping juvenile plants may assist in predicting adult plant performance. In addition, favorable wild barley alleles for growth and biomass parameters were detected, for instance, a QTL that increased biomass by approximately 36%. In particular, introgression line S42IL-121 revealed improved growth under drought stress compared to the control Scarlett. The introgression line showed a similar behavior in previous field experiments, indicating that S42IL-121 may be an attractive donor for breeding of drought tolerant barley cultivars. © 2014 Honsdorf et al.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.subjectallele
dc.subjectbarley
dc.subjectbiomass production
dc.subjectcontrolled study
dc.subjectcultivar
dc.subjectdrought tolerance
dc.subjectgene identification
dc.subjectgenetic analyzer
dc.subjectgenetic line
dc.subjectgenetic regulation
dc.subjectgrowth curve
dc.subjecthigh throughput phenotyping platform
dc.subjectintrogression
dc.subjectjuvenile plant
dc.subjectphenotype
dc.subjectplant growth
dc.subjectplant height
dc.subjectplant water use
dc.subjectquantitative trait locus
dc.subjectquantitative trait locus mapping
dc.subjectvalidation process
dc.subjectwild species
dc.titleHigh-throughput phenotyping to detect drought tolerance QTL in wild barley introgression lines
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Science Program
dc.contributor.departmentThe Salt Lab
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC4019662
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Plant Breeding, Institute of Agricultural and Nutritional Sciences, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
dc.contributor.institutionInterdisciplinary Center for Crop Plant Research (IZN), Halle (Saale), Germany
dc.contributor.institutionSchool of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Adelaide, SA, Australia
dc.contributor.institutionPlant Accelerator, University of Adelaide, Waite Campus, Adelaide, SA, Australia
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personTester, Mark A.
refterms.dateFOA2018-06-14T04:16:38Z


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