Proteomic Changes between Male and Female Worms of the Polychaetous Annelid Neanthes arenaceodentata before and after Spawning
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Computational Bioscience Research Center (CBRC)
Integrative Systems Biology Lab
KAUST Global Collaborative Research Program
Date
2013-08-30Permanent link to this record
http://hdl.handle.net/10754/325325
Metadata
Show full item recordAbstract
The Neanthes acuminata species complex (Polychaeta) are cosmopolitan in distribution. Neanthes arenaceodentata, complex, has been widely used as toxicological test animal in the marine environment. Method of reproduction is unique in this polychaete complex. Same sexes fight and opposite sexes lie side by side until egg laying. Females lose about 75% of their weight and die after laying eggs. The male, capable of reproducing up to nine times, fertilizes the eggs and incubates the embryos for 3-4 weeks. The objective of this study was to determine if there is any set of proteins that influences this unique pattern of reproduction. Gel-based two-dimensional electrophoresis (2-DE) and gel-free quantitative proteomics methods were used to identify differential protein expression patterns before and after spawning in both male and female N. arenaceodentata. Males showed a higher degree of similarity in protein expression patterns but females showed large changes in phosphoproteme before and after spawning. There was a decrease (about 70%) in the number of detected phosphoproteins in spent females. The proteins involved in muscular development, cell signaling, structure and integrity, and translation were differentially expressed. This study provides proteomic insights of the male and female worms that may serve as a foundation for better understanding of unusual reproductive patterns in polychaete worms. © 2013 Chandramouli et al.Citation
Chandramouli KH, Ravasi T, Reish D, Qian P-Y (2013) Proteomic Changes between Male and Female Worms of the Polychaetous Annelid Neanthes arenaceodentata before and after Spawning. PLoS ONE 8: e72990. doi:10.1371/journal.pone.0072990.Publisher
Public Library of Science (PLoS)Journal
PLoS ONEPubMed ID
24023665PubMed Central ID
PMC3758283ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0072990
Scopus Count
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