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dc.contributor.authorLafontant, Pascal J.
dc.contributor.authorBehzad, Ali Reza
dc.contributor.authorBrown, Evelyn
dc.contributor.authorLandry, Paul
dc.contributor.authorHu, Norman
dc.contributor.authorBurns, Alan R.
dc.date.accessioned2014-08-27T09:46:57Z
dc.date.available2014-08-27T09:46:57Z
dc.date.issued2013-08-23
dc.identifier.citationLafontant PJ, Behzad AR, Brown E, Landry P, Hu N, et al. (2013) Cardiac Myocyte Diversity and a Fibroblast Network in the Junctional Region of the Zebrafish Heart Revealed by Transmission and Serial Block-Face Scanning Electron Microscopy. PLoS ONE 8: e72388. doi:10.1371/journal.pone.0072388.
dc.identifier.issn19326203
dc.identifier.pmid24058412
dc.identifier.doi10.1371/journal.pone.0072388
dc.identifier.urihttp://hdl.handle.net/10754/325323
dc.description.abstractThe zebrafish has emerged as an important model of heart development and regeneration. While the structural characteristics of the developing and adult zebrafish ventricle have been previously studied, little attention has been paid to the nature of the interface between the compact and spongy myocardium. Here we describe how these two distinct layers are structurally and functionally integrated. We demonstrate by transmission electron microscopy that this interface is complex and composed primarily of a junctional region occupied by collagen, as well as a population of fibroblasts that form a highly complex network. We also describe a continuum of uniquely flattened transitional cardiac myocytes that form a circumferential plate upon which the radially-oriented luminal trabeculae are anchored. In addition, we have uncovered within the transitional ring a subpopulation of markedly electron dense cardiac myocytes. At discrete intervals the transitional cardiac myocytes form contact bridges across the junctional space that are stabilized through localized desmosomes and fascia adherentes junctions with adjacent compact cardiac myocytes. Finally using serial block-face scanning electron microscopy, segmentation and volume reconstruction, we confirm the three-dimensional nature of the junctional region as well as the presence of the sheet-like fibroblast network. These ultrastructural studies demonstrate the previously unrecognized complexity with which the compact and spongy layers are structurally integrated, and provide a new basis for understanding development and regeneration in the zebrafish heart. © 2013 Lafontant et al.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rightsArchived with thanks to PLoS ONE
dc.subjectactin
dc.subjectcardiac myosin
dc.subjectcollagen
dc.subjectactin filament
dc.subjectanimal cell
dc.subjectanimal tissue
dc.subjectbiodiversity
dc.subjectcaveola
dc.subjectcell interaction
dc.subjectcell junction
dc.subjectcell subpopulation
dc.subjectcell ultrastructure
dc.subjectcellular, subcellular and molecular biological phenomena and functions
dc.subjectdesmosome
dc.subjectelectron dense transitional cardiac myocyte
dc.subjectelectron translucent transitional cardiac myocyte
dc.subjectfascia
dc.subjectfibroblast network
dc.subjectfilopodium
dc.subjectheart muscle cell
dc.subjectimage reconstruction
dc.subjectphenotype
dc.subjectscanning electron microscopy
dc.subjectserial block face scanning electron microscopy
dc.subjectstructure analysis
dc.subjectthree dimensional imaging
dc.subjecttransitional cell ring
dc.subjecttransmission electron microscopy
dc.subjectultrastructure
dc.subjectzebra fish
dc.subjectCollagen
dc.subjectFibroblasts
dc.subjectMicroscopy, Electron, Scanning
dc.subjectMicroscopy, Electron, Transmission
dc.subjectMyocardium
dc.subjectMyocytes, Cardiac
dc.subjectZebrafish
dc.titleCardiac Myocyte Diversity and a Fibroblast Network in the Junctional Region of the Zebrafish Heart Revealed by Transmission and Serial Block-Face Scanning Electron Microscopy
dc.typeArticle
dc.contributor.departmentImaging and Characterization Core Lab
dc.contributor.departmentAdvanced Nanofabrication, Imaging and Characterization Core Lab
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC3751930
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Biology, DePauw University, Greencastle, IN, United States
dc.contributor.institutionCollege of Optometry, University of Houston, Houston, TX, United States
dc.contributor.institutionDepartment of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, United States
dc.contributor.institutionDepartment of Pediatrics, Baylor College of Medicine, Houston, TX, United States
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personBehzad, Ali Reza
refterms.dateFOA2018-06-13T14:57:04Z


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