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    RNA-Interference Components Are Dispensable for Transcriptional Silencing of the Drosophila Bithorax-Complex

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    Type
    Article
    Authors
    Cernilogar, Filippo M.
    Burroughs, A. Maxwell
    Lanzuolo, Chiara
    Breiling, Achim
    Imhof, Axel
    Orlando, Valerio cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    KAUST Environmental Epigenetics Research Program (KEEP)
    Date
    2013-06-13
    Permanent link to this record
    http://hdl.handle.net/10754/325319
    
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    Abstract
    Background:Beyond their role in post-transcriptional gene silencing, Dicer and Argonaute, two components of the RNA interference (RNAi) machinery, were shown to be involved in epigenetic regulation of centromeric heterochromatin and transcriptional gene silencing. In particular, RNAi mechanisms appear to play a role in repeat induced silencing and some aspects of Polycomb-mediated gene silencing. However, the functional interplay of RNAi mechanisms and Polycomb group (PcG) pathways at endogenous loci remains to be elucidated.Principal Findings:Here we show that the endogenous Dicer-2/Argonaute-2 RNAi pathway is dispensable for the PcG mediated silencing of the homeotic Bithorax Complex (BX-C). Although Dicer-2 depletion triggers mild transcriptional activation at Polycomb Response Elements (PREs), this does not induce transcriptional changes at PcG-repressed genes. Moreover, Dicer-2 is not needed to maintain global levels of methylation of lysine 27 of histone H3 and does not affect PRE-mediated higher order chromatin structures within the BX-C. Finally bioinformatic analysis, comparing published data sets of PcG targets with Argonaute-2-bound small RNAs reveals no enrichment of these small RNAs at promoter regions associated with PcG proteins.Conclusions:We conclude that the Dicer-2/Argonaute-2 RNAi pathway, despite its role in pairing sensitive gene silencing of transgenes, does not have a role in PcG dependent silencing of major homeotic gene cluster loci in Drosophila. © 2013 Cernilogar et al.
    Citation
    Cernilogar FM, Burroughs AM, Lanzuolo C, Breiling A, Imhof A, et al. (2013) RNA-Interference Components Are Dispensable for Transcriptional Silencing of the Drosophila Bithorax-Complex. PLoS ONE 8: e65740. doi:10.1371/journal.pone.0065740.
    Publisher
    Public Library of Science (PLoS)
    Journal
    PLoS ONE
    DOI
    10.1371/journal.pone.0065740
    PubMed ID
    23785447
    PubMed Central ID
    PMC3681981
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0065740
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program

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