KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Molecular Signalling Group
Visual Computing Center (VCC)
Permanent link to this recordhttp://hdl.handle.net/10754/325307
MetadataShow full item record
AbstractThe quest for the discovery of mathematical principles that underlie biological phenomena is ancient and ongoing. We present a geometric analysis of the complex interdigitated pavement cells in the Arabidopsis thaliana (Col.) adaxial epidermis with a view to discovering some geometric characteristics that may govern the formation of this tissue. More than 2,400 pavement cells from 10, 17 and 24 day old leaves were analyzed. These interdigitated cells revealed a number of geometric properties that remained constant across the three age groups. In particular, the number of digits per cell rarely exceeded 15, irrespective of cell area. Digit numbers per 100 ?m2 cell area reduce with age and as cell area increases, suggesting early developmental programming of digits. Cell shape proportions as defined by length:width ratios were highly conserved over time independent of the size and, interestingly, both the mean and the medians were close to the golden ratio 1.618034. With maturity, the cell area:perimeter ratios increased from a mean of 2.0 to 2.4. Shape properties as defined by the medial axis transform (MAT) were calculated and revealed that branch points along the MAT typically comprise one large and two small angles. These showed consistency across the developmental stages considered here at 140° (± 5°) for the largest angles and 110° (± 5°) for the smaller angles. Voronoi diagram analyses of stomatal center coordinates revealed that giant pavement cells (?500 ?m2) tend to be arranged along Voronoi boundaries suggesting that they could function as a scaffold of the epidermis. In addition, we propose that pavement cells have a role in spacing and positioning of the stomata in the growing leaf and that they do so by growing within the limits of a set of 'geometrical rules'. © 2012 Staff et al.
CitationStaff L, Hurd P, Reale L, Seoighe C, Rockwood A, et al. (2012) The Hidden Geometries of the Arabidopsis thaliana Epidermis. PLoS ONE 7: e43546. doi:10.1371/journal.pone.0043546.
PublisherPublic Library of Science (PLoS)
PubMed Central IDPMC3439452
- UV radiation reduces epidermal cell expansion in leaves of Arabidopsis thaliana.
- Authors: Hectors K, Jacques E, Prinsen E, Guisez Y, Verbelen JP, Jansen MA, Vissenberg K
- Issue date: 2010 Oct
- Differential growth of pavement cells of Arabidopsis thaliana leaf epidermis as revealed by microbead labeling.
- Authors: Elsner J, Lipowczan M, Kwiatkowska D
- Issue date: 2018 Feb
- Natural variation in stomatal abundance of Arabidopsis thaliana includes cryptic diversity for different developmental processes.
- Authors: Delgado D, Alonso-Blanco C, Fenoll C, Mena M
- Issue date: 2011 Jun
- Quantitative analysis of microtubule orientation in interdigitated leaf pavement cells.
- Authors: Akita K, Higaki T, Kutsuna N, Hasezawa S
- Issue date: 2015
- Model-based analysis of Arabidopsis leaf epidermal cells reveals distinct division and expansion patterns for pavement and guard cells.
- Authors: Asl LK, Dhondt S, Boudolf V, Beemster GT, Beeckman T, Inzé D, Govaerts W, De Veylder L
- Issue date: 2011 Aug
Showing items related by title, author, creator and subject.
Characterization of Plant Growth under Single-Wavelength Laser Light Using the Model Plant Arabidopsis ThalianaOoi, Amanda (2016-12) [Dissertation]
Advisor: Xiong, Liming
Committee members: Gehring, Christoph A; Ooi, Boon S.; Habuchi, Satoshi; Irving, HelenIndoor horticulture offers a promising solution for sustainable food production and is becoming increasingly widespread. However, it incurs high energy and cost due to the use of artificial lighting such as high-pressure sodium lamps, fluorescent light or increasingly, the light-emitting diodes (LEDs). The energy efficiency and light quality of currently available lighting is suboptimal, therefore less than ideal for sustainable and cost-effective large-scale plant production. Here, we demonstrate the use of high-powered single-wavelength lasers for indoor horticulture. Lasers are highly energy-efficient and can be remotely guided to the site of plant growth, thus reducing on-site heat accumulation. Besides, laser beams can be tailored to match the absorption profiles of different plants. We have developed a prototype laser growth chamber and demonstrate that laser-grown plants can complete a full growth cycle from seed to seed with phenotypes resembling those of plants grown under LEDs. Importantly, the plants have lower expression of proteins diagnostic for light and radiation stress. The phenotypical, biochemical and proteomic data show that the singlewavelength laser light is suitable for plant growth and therefore, potentially able to unlock the advantages of this next generation lighting technology for highly energy-efficient horticulture. Furthermore, stomatal movement partly determines the plant productivity and stress management. Abscisic acid (ABA) induces stomatal closure by promoting net K+-efflux from guard cells through outwardrectifying K+ (K+ out) channels to regulate plant water homeostasis. Here, we show that the Arabidopsis thaliana guard cell outward-rectifying K+ (ATGORK) channel is a direct target for ABA in the regulation of stomatal aperture and hence gas exchange and transpiration. Addition of (±)-ABA, but not the biologically inactive (−)-isomer, increases K+ out channel activity in Vicia faba guard cell protoplast. A similar ABA-modulated K+ channel conductance was observed when ATGORK was heterologously expressed in human embryonic kidney 293 (HEK-293) cells. Alignment of ATGORK with known PYR/PYL/RCARs ABA receptors revealed that ATGORK harbors amino acid residues that are similar to those at the latchlike region of the ABA-binding sites. In ATGORK, the double mutations K559A and Y562A at the predicted ABA-interacting site impaired ABA-dependent channel activation and reduced the affinity for ABA in vitro.
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