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dc.contributor.authorWong, Yue Him
dc.contributor.authorWang, Hao
dc.contributor.authorRavasi, Timothy
dc.contributor.authorQian, Pei-Yuan
dc.date.accessioned2014-08-27T09:45:51Z
dc.date.available2014-08-27T09:45:51Z
dc.date.issued2012-03-20
dc.identifier.citationWong YH, Wang H, Ravasi T, Qian P-Y (2012) Involvement of Wnt Signaling Pathways in the Metamorphosis of the Bryozoan Bugula neritina. PLoS ONE 7: e33323. doi:10.1371/journal.pone.0033323.
dc.identifier.issn19326203
dc.identifier.pmid22448242
dc.identifier.doi10.1371/journal.pone.0033323
dc.identifier.urihttp://hdl.handle.net/10754/325301
dc.description.abstractIn this study, we analyzed the metamorphosis of the marine bryozoan Bugula neritina. We observed the morphogenesis of the ancestrula. We defined three distinct pre-ancestrula stages based on the anatomy of the developing polypide and the overall morphology of pre-ancestrula. We then used an annotation based enrichment analysis tool to analyze the B. neritina transcriptome and identified over-representation of genes related to Wnt signaling pathways, suggesting its involvement in metamorphosis. Finally, we studied the temporal-spatial gene expression studies of several Wnt pathway genes. We found that one of the Wnt ligand, BnWnt10, was expressed spatially opposite to the Wnt antagonist BnsFRP within the blastemas, which is the presumptive polypide. Down-stream components of the canonical Wnt signaling pathway were exclusively expressed in the blastemas. Bn?catenin and BnFz5/8 were exclusively expressed in the blastemas throughout the metamorphosis. Based on the genes expression patterns, we propose that BnWnt10 and BnsFRP may relate to the patterning of the polypide, in which the two genes served as positional signals and contributed to the polarization of the blastemas. Another Wnt ligand, BnWnt6, was expressed in the apical part of the pre-ancestrula epidermis. Overall, our findings suggest that the Wnt signaling pathway may be important to the pattern formation of polypide and the development of epidermis. © 2012 Wong et al.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.rightsWong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rightsArchived with thanks to PLoS ONE
dc.subjecttranscriptome
dc.subjectbiological marker
dc.subjectmessenger RNA
dc.subjecttranscriptome
dc.subjectanimal tissue
dc.subjectbeta catenin gene
dc.subjectblastema
dc.subjectBryozoa
dc.subjectBugula neritina
dc.subjectcontrolled study
dc.subjectdevelopmental gene
dc.subjectdevelopmental stage
dc.subjectembryo pattern formation
dc.subjectFz5 8 gene
dc.subjectgene expression profiling
dc.subjectgene function
dc.subjectgene identification
dc.subjectgene location
dc.subjectmetamorphosis
dc.subjectmorphogenesis
dc.subjectWnt signaling pathway
dc.subjectWnt10 gene
dc.subjectBryozoa
dc.subjectDNA microarray
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjectin situ hybridization
dc.subjectlarva
dc.subjectmetabolism
dc.subjectreal time polymerase chain reaction
dc.subjectreverse transcription polymerase chain reaction
dc.subjectsignal transduction
dc.subjectBryozoa
dc.subjectBugula neritina
dc.subjectGymnolaemata
dc.subjectBiological Markers
dc.subjectBryozoa
dc.subjectGene Expression Regulation, Developmental
dc.subjectIn Situ Hybridization
dc.subjectLarva
dc.subjectMetamorphosis, Biological
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectSignal Transduction
dc.subjectTranscriptome
dc.subjectWnt Signaling Pathway
dc.titleInvolvement of wnt signaling pathways in the metamorphosis of the bryozoan bugula neritina
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentIntegrative Systems Biology Lab
dc.contributor.departmentKAUST Global Collaborative Research Program
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC3308966
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionUnidad Académica de Sistemas Arrecifales (Puerto Morelos), Instituto de Ciencias Del Mar y Limnología, Universidad Nacional Autõnoma de México, Puerto Morelos, QR 77580, Mexico
dc.contributor.institutionSchool of Natural Sciences, University of California Merced, 5200 North Lake Road, Merced, CA 95343, United States
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personRavasi, Timothy
refterms.dateFOA2018-06-13T14:51:42Z


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