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dc.contributor.authorChen, Zhang-Fan
dc.contributor.authorMatsumura, Kiyotaka
dc.contributor.authorWang, Hao
dc.contributor.authorArellano, Shawn M.
dc.contributor.authorYan, Xingcheng
dc.contributor.authorAlam, Intikhab
dc.contributor.authorArcher, John A.C.
dc.contributor.authorBajic, Vladimir B.
dc.contributor.authorQian, Pei-Yuan
dc.date.accessioned2014-08-27T09:45:19Z
dc.date.available2014-08-27T09:45:19Z
dc.date.issued2011-07-29
dc.identifier.citationChen Z-F, Matsumura K, Wang H, Arellano SM, Yan X, et al. (2011) Toward an Understanding of the Molecular Mechanisms of Barnacle Larval Settlement: A Comparative Transcriptomic Approach. PLoS ONE 6: e22913. doi:10.1371/journal.pone.0022913.
dc.identifier.issn19326203
dc.identifier.pmid21829555
dc.identifier.doi10.1371/journal.pone.0022913
dc.identifier.urihttp://hdl.handle.net/10754/325292
dc.description.abstractBackground: The barnacle Balanus amphitrite is a globally distributed biofouler and a model species in intertidal ecology and larval settlement studies. However, a lack of genomic information has hindered the comprehensive elucidation of the molecular mechanisms coordinating its larval settlement. The pyrosequencing-based transcriptomic approach is thought to be useful to identify key molecular changes during larval settlement. Methodology and Principal Findings: Using 454 pyrosequencing, we collected totally 630,845 reads including 215,308 from the larval stages and 415,537 from the adults; 23,451 contigs were generated while 77,785 remained as singletons. We annotated 31,720 of the 92,322 predicted open reading frames, which matched hits in the NCBI NR database, and identified 7,954 putative genes that were differentially expressed between the larval and adult stages. Of these, several genes were further characterized with quantitative real-time PCR and in situ hybridization, revealing some key findings: 1) vitellogenin was uniquely expressed in late nauplius stage, suggesting it may be an energy source for the subsequent non-feeding cyprid stage; 2) the locations of mannose receptors suggested they may be involved in the sensory system of cyprids; 3) 20 kDa-cement protein homologues were expressed in the cyprid cement gland and probably function during attachment; and 4) receptor tyrosine kinases were expressed higher in cyprid stage and may be involved in signal perception during larval settlement. Conclusions: Our results provide not only the basis of several new hypotheses about gene functions during larval settlement, but also the availability of this large transcriptome dataset in B. amphitrite for further exploration of larval settlement and developmental pathways in this important marine species. © 2011 Chen et al.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.rightsChen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rightsArchived with thanks to PLoS ONE
dc.subjectmannose receptor
dc.subjecttyrosine kinase receptor
dc.subjectvitellogenin
dc.subjectmessenger RNA
dc.subjecttranscriptome
dc.subjectbarnacle
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectcypris (larva)
dc.subjectenergy resource
dc.subjectgene expression
dc.subjectgene expression profiling
dc.subjectgene function
dc.subjectgenetic identification
dc.subjectgenetic transcription
dc.subjectin situ hybridization
dc.subjectlarva
dc.subjectlarval stage
dc.subjectmolecular mechanics
dc.subjectopen reading frame
dc.subjectprotein expression
dc.subjectpyrosequencing
dc.subjectreal time polymerase chain reaction
dc.subjectsequence homology
dc.subjectsignal detection
dc.subjecttranscriptomics
dc.subjectDNA barcoding
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjectreverse transcription polymerase chain reaction
dc.subjectThoracica
dc.subjectBalanus amphitrite
dc.subjectCyprididae
dc.subjectThoracica
dc.subjectDNA Barcoding, Taxonomic
dc.subjectLarva
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectThoracica
dc.subjectTranscriptome
dc.titleToward an understanding of the molecular mechanisms of barnacle larval settlement: A comparative transcriptomic approach
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentKAUST Global Collaborative Research Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC3146488
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionWoods Hole Oceanographic Institution, Woods Hole, MA, United States
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personAlam, Intikhab
kaust.personArcher, John A.C.
kaust.personBajic, Vladimir B.
refterms.dateFOA2018-06-14T03:38:46Z


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