Interaction between the triglyceride lipase ATGL and the arf1 activator GBF1
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AuthorsEllong, Emy Njoh
Soni, Krishnakant G.
Jackson, Catherine L.
KAUST DepartmentImaging and Characterization Core Lab
Advanced Nanofabrication, Imaging and Characterization Core Lab
MetadataShow full item record
AbstractThe Arf1 exchange factor GBF1 (Golgi Brefeldin A resistance factor 1) and its effector COPI are required for delivery of ATGL (adipose triglyceride lipase) to lipid droplets (LDs). Using yeast two hybrid, co-immunoprecipitation in mammalian cells and direct protein binding approaches, we report here that GBF1 and ATGL interact directly and in cells, through multiple contact sites on each protein. The C-terminal region of ATGL interacts with N-terminal domains of GBF1, including the catalytic Sec7 domain, but not with full-length GBF1 or its entire N-terminus. The N-terminal lipase domain of ATGL (called the patatin domain) interacts with two C-terminal domains of GBF1, HDS (Homology downstream of Sec7) 1 and HDS2. These two domains of GBF1 localize to lipid droplets when expressed alone in cells, but not to the Golgi, unlike the full-length GBF1 protein, which localizes to both. We suggest that interaction of GBF1 with ATGL may be involved in the membrane trafficking pathway mediated by GBF1, Arf1 and COPI that contributes to the localization of ATGL to lipid droplets.
CitationEllong EN, Soni KG, Bui Q-T, Sougrat R, Golinelli-Cohen M-P, et al. (2011) Interaction between the Triglyceride Lipase ATGL and the Arf1 Activator GBF1. PLoS ONE 6: e21889. doi:10.1371/journal.pone.0021889.
PublisherPublic Library of Science (PLoS)
PubMed Central IDPMC3138737
- GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis.
- Authors: Takashima K, Saitoh A, Hirose S, Nakai W, Kondo Y, Takasu Y, Kakeya H, Shin HW, Nakayama K
- Issue date: 2011
- Rab1b interacts with GBF1 and modulates both ARF1 dynamics and COPI association.
- Authors: Monetta P, Slavin I, Romero N, Alvarez C
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- Targeting of the Arf-GEF GBF1 to lipid droplets and Golgi membranes.
- Authors: Bouvet S, Golinelli-Cohen MP, Contremoulins V, Jackson CL
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- Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking.
- Authors: Szul T, Grabski R, Lyons S, Morohashi Y, Shestopal S, Lowe M, Sztul E
- Issue date: 2007 Nov 15
- Dynamics of GBF1, a Brefeldin A-sensitive Arf1 exchange factor at the Golgi.
- Authors: Niu TK, Pfeifer AC, Lippincott-Schwartz J, Jackson CL
- Issue date: 2005 Mar
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