dPORE-miRNA: Polymorphic regulation of microRNA genes
Name:
Article-PLoS_ONE-dPORE-miRN-2011.pdf
Size:
228.2Kb
Format:
PDF
Description:
Article - Full Text
Type
Article
KAUST Department
Computational Bioscience Research Center (CBRC)Date
2011-02-04Permanent link to this record
http://hdl.handle.net/10754/325287Metadata
Show full item recordAbstract
Background: MicroRNAs (miRNAs) are short non-coding RNA molecules that act as post-transcriptional regulators and affect the regulation of protein-coding genes. Mostly transcribed by PolII, miRNA genes are regulated at the transcriptional level similarly to protein-coding genes. In this study we focus on human miRNAs. These miRNAs are involved in a variety of pathways and can affect many diseases. Our interest is on possible deregulation of the transcription initiation of the miRNA encoding genes, which is facilitated by variations in the genomic sequence of transcriptional control regions (promoters). Methodology: Our aim is to provide an online resource to facilitate the investigation of the potential effects of single nucleotide polymorphisms (SNPs) on miRNA gene regulation. We analyzed SNPs overlapped with predicted transcription factor binding sites (TFBSs) in promoters of miRNA genes. We also accounted for the creation of novel TFBSs due to polymorphisms not present in the reference genome. The resulting changes in the original TFBSs and potential creation of new TFBSs were incorporated into the Dragon Database of Polymorphic Regulation of miRNA genes (dPORE-miRNA). Conclusions: The dPORE-miRNA database enables researchers to explore potential effects of SNPs on the regulation of miRNAs. dPORE-miRNA can be interrogated with regards to: a/miRNAs (their targets, or involvement in diseases, or biological pathways), b/SNPs, or c/transcription factors. dPORE-miRNA can be accessed at http://cbrc.kaust.edu.sa/dpore and http://apps.sanbi.ac.za/dpore/. Its use is free for academic and non-profit users. © 2011 Schmeier et al.Citation
Schmeier S, Schaefer U, MacPherson CR, Bajic VB (2011) dPORE-miRNA: Polymorphic Regulation of MicroRNA Genes. PLoS ONE 6: e16657. doi:10.1371/journal.pone.0016657.Publisher
Public Library of Science (PLoS)Journal
PLoS ONEISSN
19326203PubMed ID
21326606PubMed Central ID
PMC3033892Scopus Count
Related items
Showing items related by title, author, creator and subject.
-
Novel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carteri(Marine Biodiversity, Springer Nature, 2013-04-04) [Article]Despite the ubiquitous role sponges play in reef ecosystem dynamics, little is known about population-level connectivity in these organisms. The general field of population genetics in sponges remains in its infancy. To date, microsatellite markers have only been developed for few sponge species and no sponge population genetics studies using microsatellites have been conducted in the Red Sea. Here, with the use of next-generation sequencing, we characterize 12 novel polymorphic loci for the common reef sponge, Stylissa carteri. The number of alleles per loci ranged between three and eight. Observed heterozygosity frequencies (Ho) ranged from 0.125 to 0.870, whereas expected (He) heterozygosity frequencies ranged from 0.119 to 0.812. Only one locus showed consistent deviations from Hardy-Weinberg equilibrium (HWE) in both populations and two loci consistently showed the possible presence of null alleles. No significant linkage disequilibrium was detected for any pairs of loci. These microsatellites will be of use for numerous ecological studies focused on this common and abundant sponge. 2013 The Author(s).
-
A barcode of organellar genome polymorphisms identifies the geographic origin of Plasmodium falciparum strains(Nature Communications, Nature Publishing Group, 2014-06-13) [Article]Malaria is a major public health problem that is actively being addressed in a global eradication campaign. Increased population mobility through international air travel has elevated the risk of re-introducing parasites to elimination areas and dispersing drug-resistant parasites to new regions. A simple genetic marker that quickly and accurately identifies the geographic origin of infections would be a valuable public health tool for locating the source of imported outbreaks. Here we analyse the mitochondrion and apicoplast genomes of 711 Plasmodium falciparum isolates from 14 countries, and find evidence that they are non-recombining and co-inherited. The high degree of linkage produces a panel of relatively few single-nucleotide polymorphisms (SNPs) that is geographically informative. We design a 23-SNP barcode that is highly predictive (?92%) and easily adapted to aid case management in the field and survey parasite migration worldwide. 2014 Macmillan Publishers Limited. All rights reserved.
-
Lack of association between SMOC2 polymorphism and age-related macular degeneration in Jordanian Arabs(Saudi Medical Journal, Ministry of Defence And Aviation, 2012) [Article]
