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dc.contributor.authorGaravaglia, Betiana S.
dc.contributor.authorThomas, Ludivine
dc.contributor.authorGottig, Natalia
dc.contributor.authorDunger, Germán
dc.contributor.authorGarofalo, Cecilia G.
dc.contributor.authorDaurelio, Lucas D.
dc.contributor.authorNdimba, Bongani
dc.contributor.authorOrellano, Elena G.
dc.contributor.authorGehring, Christoph A
dc.contributor.authorOttado, Jorgelina
dc.date.accessioned2014-08-27T09:44:49Z
dc.date.available2014-08-27T09:44:49Z
dc.date.issued2010-01-28
dc.identifier.citationGaravaglia BS, Thomas L, Gottig N, Dunger G, Garofalo CG, et al. (2010) A Eukaryotic-Acquired Gene by a Biotrophic Phytopathogen Allows Prolonged Survival on the Host by Counteracting the Shut-Down of Plant Photosynthesis. PLoS ONE 5: e8950. doi:10.1371/journal.pone.0008950.
dc.identifier.issn19326203
dc.identifier.pmid20126632
dc.identifier.doi10.1371/journal.pone.0008950
dc.identifier.urihttp://hdl.handle.net/10754/325283
dc.description.abstractXanthomonas citri pv. citri, the bacteria responsible for citrus canker posses a biological active plant natriuretic peptide (PNP)-like protein, not present in any other bacteria. PNPs are a class of extracellular, systemically mobile peptides that elicit a number of plant responses important in homeostasis and growth. Previously, we showed that a Xanthomonas citri pv. citri mutant lacking the PNP-like protein XacPNP produced more necrotic lesions in citrus leaves than wild type infections and suggested a role for XacPNP in the regulation of host homeostasis. Here we have analyzed the proteome modifications observed in citrus leaves infected with the wild type and XacPNP deletion mutant bacteria. While both of them cause downregulation of enzymes related to photosynthesis as well as chloroplastic ribosomal proteins, proteins related to defense responses are up-regulated. However, leaves infiltrated with the XacPNP deletion mutant show a more pronounced decrease in photosynthetic proteins while no reduction in defense related proteins as compared to the wild-type pathogen. This suggests that XacPNP serves the pathogen to maintain host photosynthetic efficiency during pathogenesis. The results from the proteomics analyses are consistent with our chlorophyll fluorescence data and transcript analyses of defense genes that show a more marked reduction in photosynthesis in the mutant but no difference in the induction of genes diagnostic for biotic-stress responses. We therefore conclude that XacPNP counteracts the shut-down of host photosynthesis during infection and in that way maintains the tissue in better conditions, suggesting that the pathogen has adapted a host gene to modify its natural host and render it a better reservoir for prolonged bacterial survival and thus for further colonization. 2010 Garavaglia et al.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.rightsGaravaglia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rightsArchived with thanks to PLoS ONE
dc.subjectnatriuretic factor
dc.subjectproteome
dc.subjectribosome protein
dc.subjectvegetable protein
dc.subjectabiotic stress
dc.subjectbacterial infection
dc.subjectbacterial survival
dc.subjectbiotic stress
dc.subjectcarbon metabolism
dc.subjectchlorophyll fluorescence
dc.subjectcontrolled study
dc.subjectdeletion mutant
dc.subjectdown regulation
dc.subjecteukaryote
dc.subjecthomeostasis
dc.subjectinnate immunity
dc.subjectnatural host
dc.subjectnucleotide sequence
dc.subjectpathogenesis
dc.subjectphotosynthesis
dc.subjectplant leaf
dc.subjectplant response
dc.subjectprotein modification
dc.subjectsweet orange
dc.subjectultraviolet radiation
dc.subjectupregulation
dc.subjectXanthomonas
dc.subjectxanthomonas citri
dc.subjectCitrus
dc.subjectElectrophoresis, Gel, Two-Dimensional
dc.subjectGenes, Plant
dc.subjectHomeostasis
dc.subjectPhotosynthesis
dc.subjectPlant Leaves
dc.subjectPlant Proteins
dc.subjectProteome
dc.subjectSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
dc.subjectXanthomonas
dc.subjectBacteria (microorganisms)
dc.subjectCitrus
dc.subjectEukaryota
dc.subjectXanthomonas citri
dc.titleA eukaryotic-acquired gene by a biotrophic phytopathogen allows prolonged survival on the host by counteracting the shut-down of plant photosynthesis
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC2812515
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionMolecular Biology Division, Instituto de Biologa Molecular Y Celular de Rosario, Universidad Nacional de Rosario, Rosario, Argentina
dc.contributor.institutionConsejo de Investigaciones, Universidad Nacional de Rosario, Rosario, Argentina
dc.contributor.institutionDepartment of Biotechnology, University of the Western Cape, Bellville, South Africa
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personGehring, Christoph A.
refterms.dateFOA2018-06-13T14:47:17Z


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