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    A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors

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    Type
    Article
    Authors
    Melcher, Karsten
    Ng, Ley-Moy
    Zhou, X. Edward
    Soon, Fen-Fen
    Xu, Yong
    Suino-Powell, Kelly M.
    Park, Sang-Youl
    Weiner, Joshua J.
    Fujii, Hiroaki cc
    Chinnusamy, Viswanathan
    Kovach, Amanda
    Li, Jun
    Wang, Yonghong
    Li, Jiayang
    Peterson, Francis C.
    Jensen, Davin R.
    Yong, Eu-Leong
    Volkman, Brian F.
    Cutler, Sean R.
    Zhu, Jian-Kang cc
    Xu, H. Eric
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Center for Desert Agriculture
    Plant Stress Genomics Research Lab
    Date
    2009-12-03
    Online Publication Date
    2009-12-03
    Print Publication Date
    2009-12
    Permanent link to this record
    http://hdl.handle.net/10754/325280
    
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    Abstract
    Abscisic acid (ABA) is a ubiquitous hormone that regulates plant growth, development and responses to environmental stresses. Its action is mediated by the PYR/PYL/RCAR family of START proteins, but it remains unclear how these receptors bind ABA and, in turn, how hormone binding leads to inhibition of the downstream type 2C protein phosphatase (PP2C) effectors. Here we report crystal structures of apo and ABA-bound receptors as well as a ternary PYL2-ABA-PP2C complex. The apo receptors contain an open ligand-binding pocket flanked by a gate that closes in response to ABA by way of conformational changes in two highly conserved ?-loops that serve as a gate and latch. Moreover, ABA-induced closure of the gate creates a surface that enables the receptor to dock into and competitively inhibit the PP2C active site. A conserved tryptophan in the PP2C inserts directly between the gate and latch, which functions to further lock the receptor in a closed conformation. Together, our results identify a conserved gate-latch-lock mechanism underlying ABA signalling. © 2009 Macmillan Publishers Limited. All rights reserved.
    Citation
    Melcher K, Ng L-M, Zhou XE, Soon F-F, Xu Y, et al. (2009) A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Nature 462: 602-608. doi:10.1038/nature08613.
    Publisher
    Springer Nature
    Journal
    Nature
    DOI
    10.1038/nature08613
    PubMed ID
    19898420
    PubMed Central ID
    PMC2810868
    ae974a485f413a2113503eed53cd6c53
    10.1038/nature08613
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Center for Desert Agriculture

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