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    Assembly of the Genome of the Disease Vector Aedes aegypti onto a Genetic Linkage Map Allows Mapping of Genes Affecting Disease Transmission

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    Type
    Article
    Authors
    Juneja, Punita
    Osei-Poku, Jewelna
    Ho, Yung S.
    Ariani, Cristina V.
    Palmer, William J.
    Pain, Arnab cc
    Jiggins, Francis M.
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Computational Bioscience Research Center (CBRC)
    Date
    2014-01-30
    Permanent link to this record
    http://hdl.handle.net/10754/325279
    
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    Abstract
    The mosquito Aedes aegypti transmits some of the most important human arboviruses, including dengue, yellow fever and chikungunya viruses. It has a large genome containing many repetitive sequences, which has resulted in the genome being poorly assembled - there are 4,758 scaffolds, few of which have been assigned to a chromosome. To allow the mapping of genes affecting disease transmission, we have improved the genome assembly by scoring a large number of SNPs in recombinant progeny from a cross between two strains of Ae. aegypti, and used these to generate a genetic map. This revealed a high rate of misassemblies in the current genome, where, for example, sequences from different chromosomes were found on the same scaffold. Once these were corrected, we were able to assign 60% of the genome sequence to chromosomes and approximately order the scaffolds along the chromosome. We found that there are very large regions of suppressed recombination around the centromeres, which can extend to as much as 47% of the chromosome. To illustrate the utility of this new genome assembly, we mapped a gene that makes Ae. aegypti resistant to the human parasite Brugia malayi, and generated a list of candidate genes that could be affecting the trait. © 2014 Juneja et al.
    Citation
    Juneja P, Osei-Poku J, Ho YS, Ariani CV, Palmer WJ, et al. (2014) Assembly of the Genome of the Disease Vector Aedes aegypti onto a Genetic Linkage Map Allows Mapping of Genes Affecting Disease Transmission. PLoS Negl Trop Dis 8: e2652. doi:10.1371/journal.pntd.0002652.
    Publisher
    Public Library of Science (PLoS)
    Journal
    PLoS Neglected Tropical Diseases
    DOI
    10.1371/journal.pntd.0002652
    PubMed ID
    24498447
    PubMed Central ID
    PMC3907309
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pntd.0002652
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Computational Bioscience Research Center (CBRC)

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