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dc.contributor.authorChen, Tao
dc.contributor.authorCui, Peng
dc.contributor.authorChen, Hao
dc.contributor.authorAli, Shahjahan
dc.contributor.authorZhang, ShouDong
dc.contributor.authorXiong, Liming
dc.date.accessioned2014-08-27T09:44:34Z
dc.date.available2014-08-27T09:44:34Z
dc.date.issued2013-10-17
dc.identifier.citationChen T, Cui P, Chen H, Ali S, Zhang S, et al. (2013) A KH-Domain RNA-Binding Protein Interacts with FIERY2/CTD Phosphatase-Like 1 and Splicing Factors and Is Important for Pre-mRNA Splicing in Arabidopsis. PLoS Genet 9: e1003875. doi:10.1371/journal.pgen.1003875.
dc.identifier.issn15537390
dc.identifier.pmid24146632
dc.identifier.doi10.1371/journal.pgen.1003875
dc.identifier.urihttp://hdl.handle.net/10754/325278
dc.description.abstractEukaryotic genomes encode hundreds of RNA-binding proteins, yet the functions of most of these proteins are unknown. In a genetic study of stress signal transduction in Arabidopsis, we identified a K homology (KH)-domain RNA-binding protein, HOS5 (High Osmotic Stress Gene Expression 5), as required for stress gene regulation and stress tolerance. HOS5 was found to interact with FIERY2/RNA polymerase II (RNAP II) carboxyl terminal domain (CTD) phosphatase-like 1 (FRY2/CPL1) both in vitro and in vivo. This interaction is mediated by the first double-stranded RNA-binding domain of FRY2/CPL1 and the KH domains of HOS5. Interestingly, both HOS5 and FRY2/CPL1 also interact with two novel serine-arginine (SR)-rich splicing factors, RS40 and RS41, in nuclear speckles. Importantly, FRY2/CPL1 is required for the recruitment of HOS5. In fry2 mutants, HOS5 failed to be localized in nuclear speckles but was found mainly in the nucleoplasm. hos5 mutants were impaired in mRNA export and accumulated a significant amount of mRNA in the nuclei, particularly under salt stress conditions. Arabidopsis mutants of all these genes exhibit similar stress-sensitive phenotypes. RNA-seq analyses of these mutants detected significant intron retention in many stress-related genes under salt stress but not under normal conditions. Our study not only identified several novel regulators of pre-mRNA processing as important for plant stress response but also suggested that, in addition to RNAP II CTD that is a well-recognized platform for the recruitment of mRNA processing factors, FRY2/CPL1 may also recruit specific factors to regulate the co-transcriptional processing of certain transcripts to deal with environmental challenges. © 2013 Chen et al.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rightsArchived with thanks to PLoS Genetics
dc.subjectdouble stranded RNA
dc.subjectFIERY2 RNA polymerase 2 carboxyl terminal domain phosphatase like 1 potein
dc.subjecthigh osmotic stress gene expression 5
dc.subjectK homology domain RNA binding protein
dc.subjectmembrane protein
dc.subjectRNA binding protein
dc.subjectRNA polymerase II
dc.subjectunclassified drug
dc.subjectamino terminal sequence
dc.subjectArabidopsis
dc.subjectcarboxy terminal sequence
dc.subjectcontrolled study
dc.subjectenvironmental stress
dc.subjectgene control
dc.subjectgene frequency
dc.subjectgene mapping
dc.subjectgenetic transcription
dc.subjectgenetic transformation
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectintron
dc.subjectmolecular recognition
dc.subjectphenotype
dc.subjectplant growth
dc.subjectpolyadenylation
dc.subjectpromoter region
dc.subjectprotein binding
dc.subjectprotein localization
dc.subjectprotein phosphorylation
dc.subjectprotein protein interaction
dc.subjectprotein structure
dc.subjectprotoplast
dc.subjectreporter gene
dc.subjectreverse transcription polymerase chain reaction
dc.subjectRNA sequence
dc.subjectRNA splicing
dc.subjectsalt stress
dc.subjectsignal transduction
dc.subjectstress
dc.subjectArabidopsis
dc.subjectArabidopsis Proteins
dc.subjectCell Nucleus
dc.subjectGene Expression Regulation, Plant
dc.subjectMutation
dc.subjectPhosphoprotein Phosphatases
dc.subjectProtein Binding
dc.subjectProtein Structure, Tertiary
dc.subjectRNA Precursors
dc.subjectRNA Splicing
dc.subjectRNA, Double-Stranded
dc.subjectRNA-Binding Proteins
dc.subjectSalinity
dc.subjectSignal Transduction
dc.subjectTranscription Factors
dc.subjectTranscription, Genetic
dc.titleA KH-Domain RNA-Binding Protein Interacts with FIERY2/CTD Phosphatase-Like 1 and Splicing Factors and Is Important for Pre-mRNA Splicing in Arabidopsis
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Core Lab
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Stress Genomics Research Lab
dc.identifier.journalPLoS Genetics
dc.identifier.pmcidPMC3798263
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionUnidad Académica de Sistemas Arrecifales (Puerto Morelos), Instituto de Ciencias Del Mar y Limnología, Universidad Nacional Autõnoma de México, Puerto Morelos, QR 77580, Mexico
dc.contributor.institutionSchool of Natural Sciences, University of California Merced, 5200 North Lake Road, Merced, CA 95343, United States
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personCui, Peng
kaust.personAli, Shahjahan
kaust.personZhang, ShouDong
kaust.personXiong, Liming
kaust.personChen, Tao
kaust.personChen, Hao
refterms.dateFOA2018-06-14T03:47:18Z


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