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dc.contributor.authorCui, Peng
dc.contributor.authorZhang, ShouDong
dc.contributor.authorDing, Feng
dc.contributor.authorAli, Shahjahan
dc.contributor.authorXiong, Liming
dc.date.accessioned2014-08-27T09:44:09Z
dc.date.available2014-08-27T09:44:09Z
dc.date.issued2014-01-07
dc.identifier.citationCui P, Zhang S, Ding F, Ali S, Xiong L (2014) Dynamic regulation of genome-wide pre-mRNA splicing and stress tolerance by the Sm-like protein LSm5 in Arabidopsis. Genome Biology 15: R1. doi:10.1186/gb-2014-15-1-r1.
dc.identifier.issn1474760X
dc.identifier.pmid24393432
dc.identifier.doi10.1186/gb-2014-15-1-r1
dc.identifier.urihttp://hdl.handle.net/10754/325272
dc.description.abstractBackground: Sm-like proteins are highly conserved proteins that form the core of the U6 ribonucleoprotein and function in several mRNA metabolism processes, including pre-mRNA splicing. Despite their wide occurrence in all eukaryotes, little is known about the roles of Sm-like proteins in the regulation of splicing.Results: Here, through comprehensive transcriptome analyses, we demonstrate that depletion of the Arabidopsis supersensitive to abscisic acid and drought 1 gene (SAD1), which encodes Sm-like protein 5 (LSm5), promotes an inaccurate selection of splice sites that leads to a genome-wide increase in alternative splicing. In contrast, overexpression of SAD1 strengthens the precision of splice-site recognition and globally inhibits alternative splicing. Further, SAD1 modulates the splicing of stress-responsive genes, particularly under salt-stress conditions. Finally, we find that overexpression of SAD1 in Arabidopsis improves salt tolerance in transgenic plants, which correlates with an increase in splicing accuracy and efficiency for stress-responsive genes.Conclusions: We conclude that SAD1 dynamically controls splicing efficiency and splice-site recognition in Arabidopsis, and propose that this may contribute to SAD1-mediated stress tolerance through the metabolism of transcripts expressed from stress-responsive genes. Our study not only provides novel insights into the function of Sm-like proteins in splicing, but also uncovers new means to improve splicing efficiency and to enhance stress tolerance in a higher eukaryote. 2014 Cui et al.; licensee BioMed Central Ltd.
dc.language.isoen
dc.publisherSpringer Nature
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.subjectArabidopsis protein
dc.subjectLSm5 protein
dc.subjectmessenger RNA precursor
dc.subjectSm like protein
dc.subjectunclassified drug
dc.subjectalternative RNA splicing
dc.subjectArabidopsis
dc.subjectcontrolled study
dc.subjectexon
dc.subjectgene mapping
dc.subjectgene overexpression
dc.subjectintron
dc.subjectphenotype
dc.subjectplant gene
dc.subjectplant genome
dc.subjectplant stress
dc.subjectprotein function
dc.subjectreverse transcription polymerase chain reaction
dc.subjectRNA sequence
dc.subjectRNA splicing
dc.subjectsalt stress
dc.subjectsalt tolerance
dc.subjectsequence alignment
dc.subjectsequence analysis
dc.subjectsupersensitive to abscisic acid and drought 1 gene
dc.subjecttransgenic plant
dc.titleDynamic regulation of genome-wide pre-mRNA splicing and stress tolerance by the Sm-like protein LSm5 in Arabidopsis
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalGenome Biology
dc.identifier.pmcidPMC4053965
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionUnidad Académica de Sistemas Arrecifales (Puerto Morelos), Instituto de Ciencias Del Mar y Limnología, Universidad Nacional Autõnoma de México, Puerto Morelos, QR 77580, Mexico
dc.contributor.institutionSchool of Natural Sciences, University of California Merced, 5200 North Lake Road, Merced, CA 95343, United States
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personCui, Peng
kaust.personZhang, ShouDong
kaust.personDing, Feng
kaust.personAli, Shahjahan
kaust.personXiong, Liming
refterms.dateFOA2018-06-14T03:46:48Z


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This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.