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dc.contributor.authorMeier, Stuart
dc.contributor.authorTzfadia, Oren
dc.contributor.authorVallabhaneni, Ratnakar
dc.contributor.authorGehring, Christoph A
dc.contributor.authorWurtzel, Eleanore T
dc.date.accessioned2014-08-27T09:43:46Z
dc.date.available2014-08-27T09:43:46Z
dc.date.issued2011-05-19
dc.identifier.citationMeier S, Tzfadia O, Vallabhaneni R, Gehring C, Wurtzel ET (2011) A transcriptional analysis of carotenoid, chlorophyll and plastidial isoprenoid biosynthesis genes during development and osmotic stress responses in Arabidopsis thaliana. BMC Systems Biology 5: 77. doi:10.1186/1752-0509-5-77.
dc.identifier.issn17520509
dc.identifier.pmid21595952
dc.identifier.doi10.1186/1752-0509-5-77
dc.identifier.urihttp://hdl.handle.net/10754/325268
dc.description.abstractBackground: The carotenoids are pure isoprenoids that are essential components of the photosynthetic apparatus and are coordinately synthesized with chlorophylls in chloroplasts. However, little is known about the mechanisms that regulate carotenoid biosynthesis or the mechanisms that coordinate this synthesis with that of chlorophylls and other plastidial synthesized isoprenoid-derived compounds, including quinones, gibberellic acid and abscisic acid. Here, a comprehensive transcriptional analysis of individual carotenoid and isoprenoid-related biosynthesis pathway genes was performed in order to elucidate the role of transcriptional regulation in the coordinated synthesis of these compounds and to identify regulatory components that may mediate this process in Arabidopsis thaliana.Results: A global microarray expression correlation analysis revealed that the phytoene synthase gene, which encodes the first dedicated and rate-limiting enzyme of carotenogenesis, is highly co-expressed with many photosynthesis-related genes including many isoprenoid-related biosynthesis pathway genes. Chemical and mutant analysis revealed that induction of the co-expressed genes following germination was dependent on gibberellic acid and brassinosteroids (BR) but was inhibited by abscisic acid (ABA). Mutant analyses further revealed that expression of many of the genes is suppressed in dark grown plants by Phytochrome Interacting transcription Factors (PIFs) and activated by photoactivated phytochromes, which in turn degrade PIFs and mediate a coordinated induction of the genes. The promoters of PSY and the co-expressed genes were found to contain an enrichment in putative BR-auxin response elements and G-boxes, which bind PIFs, further supporting a role for BRs and PIFs in regulating expression of the genes. In osmotically stressed root tissue, transcription of Calvin cycle, methylerythritol 4-phosphate pathway and carotenoid biosynthesis genes is induced and uncoupled from that of chlorophyll biosynthesis genes in a manner that is consistent with the increased synthesis of carotenoid precursors for ABA biosynthesis. In all tissues examined, induction of ?-carotene hydroxylase transcript levels are linked to an increased demand for ABA.Conclusions: This analysis provides compelling evidence to suggest that coordinated transcriptional regulation of isoprenoid-related biosynthesis pathway genes plays a major role in coordinating the synthesis of functionally related chloroplast localized isoprenoid-derived compounds. 2011 Meier et al; licensee BioMed Central Ltd.
dc.language.isoen
dc.publisherSpringer Nature
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.subjectArabidopsis
dc.subjectArabidopsis thaliana
dc.subjectabscisic acid
dc.subjectbeta carotene
dc.subjectcarotenoid
dc.subjectchlorophyll
dc.subjectterpene
dc.subjectArabidopsis
dc.subjectbiological model
dc.subjectbiology
dc.subjectchemistry
dc.subjectchloroplast
dc.subjectDNA microarray
dc.subjectgene expression regulation
dc.subjectgenetic transcription
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmethodology
dc.subjectmutation
dc.subjectosmosis
dc.subjectplastid
dc.subjectpromoter region
dc.subjectsystems biology
dc.subjectAbscisic Acid
dc.subjectArabidopsis
dc.subjectbeta Carotene
dc.subjectCarotenoids
dc.subjectChlorophyll
dc.subjectChloroplasts
dc.subjectComputational Biology
dc.subjectGene Expression Regulation, Plant
dc.subjectModels, Biological
dc.subjectMutation
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectOsmosis
dc.subjectPlastids
dc.subjectPromoter Regions, Genetic
dc.subjectSystems Biology
dc.subjectTerpenes
dc.subjectTranscription, Genetic
dc.titleA transcriptional analysis of carotenoid, chlorophyll and plastidial isoprenoid biosynthesis genes during development and osmotic stress responses in Arabidopsis thaliana
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalBMC Systems Biology
dc.identifier.pmcidPMC3123201
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Biological Sciences, Lehman College, The City University of New York, 250 Bedford Park Blvd. West, Bronx, NY 10468, United States
dc.contributor.institutionThe Graduate School and University Center-CUNY, 365 Fifth Ave., New York, NY 10016-4309, United States
dc.contributor.institutionDepartment of Biotechnology, University of the Western Cape, Private Bag X17, Cape Town - Bellville 7535, South Africa
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personMeier, Stuart Kurt
kaust.personGehring, Christoph A
refterms.dateFOA2018-06-13T14:42:26Z


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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.