Molecular characterization of a naturally occurring intraspecific recombinant begomovirus with close relatives widespread in southern Arabia
Name:
Article-Virology_J-Molecular_-2014.pdf
Size:
1.171Mb
Format:
PDF
Description:
Article - Full Text
Name:
Supplement_1_-_Virology_J-Molecular_-2014.1743-422X-11-103-S1.docx
Size:
18.65Kb
Format:
Microsoft Word 2007
Description:
Supplemental File 1
Name:
Supplement_2_-_Virology_J-Molecular_-2014.1743-422X-11-103-S2.pptx
Size:
681.2Kb
Format:
Microsoft PowerPoint 2007
Description:
Supplemental File 2
Name:
Supplement_3_-_Virology_J-Molecular_-2014.1743-422X-11-103-S3.pptx
Size:
79.47Kb
Format:
Microsoft PowerPoint 2007
Description:
Supplemental File 3
Name:
Supplement_4_-_Virology_J-Molecular_-2014.1743-422X-11-103-S4.pptx
Size:
564.4Kb
Format:
Microsoft PowerPoint 2007
Description:
Supplemental File 4
Name:
Supplement_5_-_Virology_J-Molecular_-2014.1743-422X-11-103-S5.pptx
Size:
547.1Kb
Format:
Microsoft PowerPoint 2007
Description:
Supplemental File 5
Type
ArticleKAUST Department
Center for Desert AgricultureDate
2014-06-02Online Publication Date
2014-06-02Print Publication Date
2014Permanent link to this record
http://hdl.handle.net/10754/325262
Metadata
Show full item recordAbstract
Background: Tomato leaf curl Sudan virus (ToLCSDV) is a single-stranded DNA begomovirus of tomato that causes downward leaf curl, yellowing, and stunting. Leaf curl disease results in significant yield reduction in tomato crops in the Nile Basin. ToLCSDV symptoms resemble those caused by Tomato yellow leaf curl virus, a distinct and widespread begomovirus originating in the Middle East. In this study, tomato samples exhibiting leaf curl symptoms were collected from Gezira, Sudan. The associated viral genome was molecularly characterized, analyzed phylogenetically, and an infectious clone for one isolate was constructed. Findings. The complete genomes for five newly discovered variants of ToLCSDV, ranging in size from 2765 to 2767-bp, were cloned and sequenced, and subjected to pairwise and phylogenetic analyses. Pairwise analysis indicated that the five Gezira isolates shared 97-100% nucleotide identity with each other. Further, these variants of ToLCSDV shared their highest nucleotide identity at 96-98%, 91-95%, 91-92%, and 91-92% with the Shambat, Gezira, Oman and Yemen strains of ToLCSDV, respectively. Based on the high maximum nucleotide identities shared between these ToLCSDV variants from Gezira and other previously recognized members of this taxonomic group, they are considered isolates of the Shambat strain of ToLCSDV. Analysis of the complete genome sequence for these new variants revealed that they were naturally occurring recombinants between two previously reported strains of ToLCSDV. Finally, a dimeric clone constructed from one representative ToLCSV genome from Gezira was shown to be infectious following inoculation to tomato and N. benthamiana plants. Conclusion: Five new, naturally occurring recombinant begomovirus variants (>96% shared nt identity) were identified in tomato plants from Gezira in Sudan, and shown to be isolates of the Shambat strain of ToLCSDV. The cloned viral genome was infectious in N. benthamiana and tomato plants, and symptoms in tomato closely resembled those observed in field infected tomato plants, indicating the virus is the causal agent of the leaf curl disease. The symptoms that developed in tomato seedlings closely resembled those observed in field infected tomato plants, indicating that ToLCSDV is the causal agent of the leaf curl disease in Gezira. 2014 Al-Saleh et al.; licensee BioMed Central Ltd.Citation
Al-Saleh MA, Al-Shahwan IM, Brown JK, Idris AM (2014) Molecular characterization of a naturally occurring intraspecific recombinant begomovirus with close relatives widespread in southern Arabia. Virology Journal 11: 103. doi:10.1186/1743-422X-11-103.Publisher
Springer NatureJournal
Virology JournalPubMed ID
24890736PubMed Central ID
PMC4071017ae974a485f413a2113503eed53cd6c53
10.1186/1743-422X-11-103
Scopus Count
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
Related articles
- Leaf curl diseases of two solanaceous species in Southwest Arabia are caused by a monopartite begomovirus evolutionarily most closely related to a species from the Nile Basin and unique suite of betasatellites.
- Authors: Idris AM, Abdullah NM, Brown JK
- Issue date: 2012 Oct
- Cotton leaf curl Gezira alphasatellite associated with tomato leaf curl Sudan virus approaches the expected upper size limit in the evolution of alphasatellites.
- Authors: Fiallo-Olivé E, Hamed A, Navas-Castillo J, Moriones E
- Issue date: 2013 Dec 26
- Evidence for interspecific-recombination for three monopartite begomoviral genomes associated with the tomato leaf curl disease from central Sudan.
- Authors: Idris AM, Brown JK
- Issue date: 2005 May
- Recent evolution of a novel begomovirus causing tomato leaf curl disease in the Al-Batinah region of Oman.
- Authors: Khan AJ, Akhtar S, Singh AK, Al-Shehi AA, Al-Matrushi AM, Ammara U, Briddon RW
- Issue date: 2014 Mar
- Molecular and biological characterization of Chilli leaf curl virus and associated Tomato leaf curl betasatellite infecting tobacco in Oman.
- Authors: Shahid MS, Shafiq M, Raza A, Al-Sadi AM, Briddon RW
- Issue date: 2019 Nov 9