The response and recovery of the Arabidopsis thaliana transcriptome to phosphate starvation
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MacPherson, Cameron Ross
Hannah, Matthew A
Bajic, Vladimir B.
KAUST DepartmentComputational Bioscience Research Center (CBRC)
MetadataShow full item record
AbstractBackground: Over application of phosphate fertilizers in modern agriculture contaminates waterways and disrupts natural ecosystems. Nevertheless, this is a common practice among farmers, especially in developing countries as abundant fertilizers are believed to boost crop yields. The study of plant phosphate metabolism and its underlying genetic pathways is key to discovering methods of efficient fertilizer usage. The work presented here describes a genome-wide resource on the molecular dynamics underpinning the response and recovery in roots and shoots of Arabidopsis thaliana to phosphate-starvation.Results: Genome-wide profiling by micro- and tiling-arrays (accessible from GEO: GSE34004) revealed minimal overlap between root and shoot transcriptomes suggesting two independent phosphate-starvation regulons. Novel gene expression patterns were detected for over 1000 candidates and were classified as either initial, persistent, or latent responders. Comparative analysis to AtGenExpress identified cohorts of genes co-regulated across multiple stimuli. The hormone ABA displayed a dominant role in regulating many phosphate-responsive candidates. Analysis of co-regulation enabled the determination of specific versus generic members of closely related gene families with respect to phosphate-starvation. Thus, among others, we showed that PHR1-regulated members of closely related phosphate-responsive families (PHT1;1, PHT1;7-9, SPX1-3, and PHO1;H1) display greater specificity to phosphate-starvation than their more generic counterparts. Conclusion: Our results uncover much larger, staged responses to phosphate-starvation than previously described. To our knowledge, this work describes the most complete genome-wide data on plant nutrient stress to-date. 2012 Woo et al.; licensee BioMed Central Ltd.
CitationWoo J, MacPherson C, Liu J, Wang H, Kiba T, et al. (2012) The response and recovery of the Arabidopsis thaliana transcriptome to phosphate starvation. BMC Plant Biol 12: 62. doi:10.1186/1471-2229-12-62.
JournalBMC Plant Biology
PubMed Central IDPMC3520718
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Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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- Issue date: 2012 Jul 23
- The transcription factor PHR1 plays a key role in the regulation of sulfate shoot-to-root flux upon phosphate starvation in Arabidopsis.
- Authors: Rouached H, Secco D, Arpat B, Poirier Y
- Issue date: 2011 Jan 24
- Root Cell-Specific Regulators of Phosphate-Dependent Growth.
- Authors: Linn J, Ren M, Berkowitz O, Ding W, van der Merwe MJ, Whelan J, Jost R
- Issue date: 2017 Jul
- Arabidopsis Pht1;5 mobilizes phosphate between source and sink organs and influences the interaction between phosphate homeostasis and ethylene signaling.
- Authors: Nagarajan VK, Jain A, Poling MD, Lewis AJ, Raghothama KG, Smith AP
- Issue date: 2011 Jul
- A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis.
- Authors: Bustos R, Castrillo G, Linhares F, Puga MI, Rubio V, Pérez-Pérez J, Solano R, Leyva A, Paz-Ares J
- Issue date: 2010 Sep 9
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