Show simple item record

dc.contributor.authorZimaro, Tamara
dc.contributor.authorThomas, Ludivine
dc.contributor.authorMarondedze, Claudius
dc.contributor.authorSgro, Germán G
dc.contributor.authorGarofalo, Cecilia G
dc.contributor.authorFicarra, Florencia A
dc.contributor.authorGehring, Christoph A
dc.contributor.authorOttado, Jorgelina
dc.contributor.authorGottig, Natalia
dc.date.accessioned2014-08-27T09:42:18Z
dc.date.available2014-08-27T09:42:18Z
dc.date.issued2014-04-18
dc.identifier.citationZimaro T, Thomas L, Marondedze C, Sgro GG, Garofalo CG, et al. (2014) The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation. BMC Microbiology 14: 96. doi:10.1186/1471-2180-14-96.
dc.identifier.issn14712180
dc.identifier.pmid24742141
dc.identifier.doi10.1186/1471-2180-14-96
dc.identifier.urihttp://hdl.handle.net/10754/325248
dc.description.abstractBackground: Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein secretion system (T3SS). The role of T3SS in bacterial pathogenesis is well established but whether this system is involved in multicellular processes, such as bacterial biofilm formation has not been elucidated. Here, the phytopathogen Xanthomonas citri subsp. citri (X. citri) was used as a model to gain further insights about the role of the T3SS in biofilm formation. Results: The capacity of biofilm formation of different X. citri T3SS mutants was compared to the wild type strain and it was observed that this secretion system was necessary for this process. Moreover, the T3SS mutants adhered proficiently to leaf surfaces but were impaired in leaf-associated growth. A proteomic study of biofilm cells showed that the lack of the T3SS causes changes in the expression of proteins involved in metabolic processes, energy generation, exopolysaccharide (EPS) production and bacterial motility as well as outer membrane proteins. Furthermore, EPS production and bacterial motility were also altered in the T3SS mutants. Conclusions: Our results indicate a novel role for T3SS in X. citri in the modulation of biofilm formation. Since this process increases X. citri virulence, this study reveals new functions of T3SS in pathogenesis. 2014 Zimaro et al.; licensee BioMed Central Ltd.
dc.language.isoen
dc.publisherSpringer Nature
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.subjectBiofilm
dc.subjectProteomics
dc.subjectT3SS
dc.subjectXanthomonas citri subsp. citri
dc.subjectbacterial protein
dc.subjectexopolysaccharide
dc.subjectouter membrane protein
dc.subjectbacterial cell
dc.subjectbacterial metabolism
dc.subjectbacterium adherence
dc.subjectbacterium mutant
dc.subjectbiofilm
dc.subjectcarbohydrate synthesis
dc.subjectcontrolled study
dc.subjectenergy yield
dc.subjectleaf surface
dc.subjectprotein expression
dc.subjectprotein secretion
dc.subjectproteomics
dc.subjecttype III protein secretion system
dc.subjecttype III secretion system
dc.subjectwild type
dc.subjectXanthomonas citri
dc.subjectBacteria (microorganisms)
dc.subjectXanthomonas citri
dc.titleThe type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Core Lab
dc.contributor.departmentBioscience Program
dc.contributor.departmentMolecular Signalling Group
dc.identifier.journalBMC Microbiology
dc.identifier.pmcidPMC4021560
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstituto de Biologa Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Cientficas y Tcnicas (IBR-CONICET), Universidad Nacional de Rosario. Ocampo y Esmeralda, Rosario 2000, Argentina
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personThomas, Ludivine
kaust.personMarondedze, Claudius
kaust.personGehring, Christoph A.
refterms.dateFOA2018-06-14T03:46:30Z
dc.date.published-online2014-04-18
dc.date.published-print2014


Files in this item

Thumbnail
Name:
Article-BMC_Microb-The_type_I-2014.pdf
Size:
2.227Mb
Format:
PDF
Description:
Article - Full Text
Thumbnail
Name:
Supplement_1_-_BMC_Microb-The_type_I-2014.1471-2180-14-96-S1.pdf
Size:
137.0Kb
Format:
PDF
Description:
Supplemental File 1
Thumbnail
Name:
Supplement_2_-_BMC_Microb-The_type_I-2014.1471-2180-14-96-S2.pdf
Size:
818.8Kb
Format:
PDF
Description:
Supplemental File 2
Thumbnail
Name:
Supplement_3_-_BMC_Microb-The_type_I-2014.1471-2180-14-96-S3.pdf
Size:
7.070Kb
Format:
PDF
Description:
Supplemental File 3

This item appears in the following Collection(s)

Show simple item record

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.