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Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein

dc.contributor.authorMahfouz, Magdy M.
dc.contributor.authorLi, Lixin
dc.contributor.authorPiatek, Marek J.
dc.contributor.authorFang, Xiaoyun
dc.contributor.authorMansour, Hicham
dc.contributor.authorBangarusamy, Dhinoth Kumar
dc.contributor.authorZhu, Jian-Kang
dc.date.accessioned2014-08-27T09:42:15Z
dc.date.available2014-08-27T09:42:15Z
dc.date.issued2011-12-14
dc.identifier.citationMahfouz MM, Li L, Piatek M, Fang X, Mansour H, et al. (2011) Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein. Plant Molecular Biology 78: 311-321. doi:10.1007/s11103-011-9866-x.
dc.identifier.issn01674412
dc.identifier.pmid22167390
dc.identifier.doi10.1007/s11103-011-9866-x
dc.identifier.urihttp://hdl.handle.net/10754/325247
dc.description.abstractTranscriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3. SRDX protein efficiently repressed the transcription of the RD29A
dc.language.isoen
dc.publisherSpringer Nature
dc.rights© The Author(s) 2011
dc.rightsArchived with thanks to Plant Molecular Biology
dc.subjectChimeric repressors
dc.subjectEAR-repression domain
dc.subjectTALE-based repressors
dc.subjectTargeted repression
dc.subjectArabidopsis protein
dc.subjecthybrid protein
dc.subjectplant DNA
dc.subjectRD29a protein, Arabidopsis
dc.subjectrepressor protein
dc.subjectArabidopsis
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmolecular genetics
dc.subjectnucleotide sequence
dc.subjectplant gene
dc.subjectpromoter region
dc.subjecttransgenic plant
dc.subjectArabidopsis
dc.subjectArabidopsis Proteins
dc.subjectBase Sequence
dc.subjectDNA, Plant
dc.subjectGenes, Plant
dc.subjectMolecular Sequence Data
dc.subjectPlants, Genetically Modified
dc.subjectPromoter Regions, Genetic
dc.subjectRecombinant Fusion Proteins
dc.subjectRepressor Proteins
dc.subjectArabidopsis
dc.subjectBacteria (microorganisms)
dc.subjectMammalia
dc.subjectXanthomonas
dc.titleTargeted transcriptional repression using a chimeric TALE-SRDX repressor protein
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Core Lab
dc.contributor.departmentBioscience Program
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Stress Genomics Research Lab
dc.identifier.journalPlant Molecular Biology
dc.identifier.pmcidPMC3259320
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personMahfouz, Magdy M.
kaust.personLi, Lixin
kaust.personMansour, Hicham
kaust.personBangarusamy, Dhinoth Kumar
kaust.personPiatek, Marek J.
kaust.personFang, Xiaoyun
refterms.dateFOA2018-06-13T14:36:32Z
dc.date.published-online2011-12-14
dc.date.published-print2012-02


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