Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein
Type
ArticleAuthors
Mahfouz, Magdy M.
Li, Lixin
Piatek, Marek J.
Fang, Xiaoyun
Mansour, Hicham
Bangarusamy, Dhinoth K.
Zhu, Jian-Kang
KAUST Department
Plant Stress Genomics Research LabDate
2011-12-14Metadata
Show full item recordAbstract
Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3. SRDX protein efficiently repressed the transcription of the RD29ACitation
Mahfouz MM, Li L, Piatek M, Fang X, Mansour H, et al. (2011) Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein. Plant Molecular Biology 78: 311-321. doi:10.1007/s11103-011-9866-x.Publisher
Springer NatureJournal
Plant Molecular BiologyISSN
01674412PubMed ID
22167390PubMed Central ID
PMC3259320Handle URI
http://hdl.handle.net/10754/325247Scopus Count
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