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    Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein

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    Type
    Article
    Authors
    Mahfouz, Magdy M. cc
    Li, Lixin
    Piatek, Marek J. cc
    Fang, Xiaoyun
    Mansour, Hicham
    Bangarusamy, Dhinoth Kumar
    Zhu, Jian-Kang cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Core Lab
    Bioscience Program
    Center for Desert Agriculture
    Laboratory for Genome Engineering
    Plant Science
    Plant Stress Genomics Research Lab
    Date
    2011-12-14
    Online Publication Date
    2011-12-14
    Print Publication Date
    2012-02
    Permanent link to this record
    http://hdl.handle.net/10754/325247
    
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    Abstract
    Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3. SRDX protein efficiently repressed the transcription of the RD29A
    Citation
    Mahfouz MM, Li L, Piatek M, Fang X, Mansour H, et al. (2011) Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein. Plant Molecular Biology 78: 311-321. doi:10.1007/s11103-011-9866-x.
    Publisher
    Springer Nature
    Journal
    Plant Molecular Biology
    DOI
    10.1007/s11103-011-9866-x
    PubMed ID
    22167390
    PubMed Central ID
    PMC3259320
    ae974a485f413a2113503eed53cd6c53
    10.1007/s11103-011-9866-x
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Bioscience Core Lab; Center for Desert Agriculture

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