Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein
AuthorsMahfouz, Magdy M.
Piatek, Marek J.
Bangarusamy, Dhinoth Kumar
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Bioscience Core Lab
Center for Desert Agriculture
Laboratory for Genome Engineering
Plant Stress Genomics Research Lab
Online Publication Date2011-12-14
Print Publication Date2012-02
Permanent link to this recordhttp://hdl.handle.net/10754/325247
MetadataShow full item record
AbstractTranscriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3. SRDX protein efficiently repressed the transcription of the RD29A
CitationMahfouz MM, Li L, Piatek M, Fang X, Mansour H, et al. (2011) Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein. Plant Molecular Biology 78: 311-321. doi:10.1007/s11103-011-9866-x.
JournalPlant Molecular Biology
PubMed Central IDPMC3259320
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