Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein
Type
ArticleAuthors
Mahfouz, Magdy M.
Li, Lixin
Piatek, Marek J.
Fang, Xiaoyun
Mansour, Hicham
Bangarusamy, Dhinoth Kumar
Zhu, Jian-Kang
KAUST Department
Bioscience Core LabBioscience Program
Desert Agriculture Initiative
Laboratory for Genome Engineering
Plant Science
Plant Stress Genomics Research Lab
Date
2011-12-14Online Publication Date
2011-12-14Print Publication Date
2012-02Permanent link to this record
http://hdl.handle.net/10754/325247Metadata
Show full item recordAbstract
Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3. SRDX protein efficiently repressed the transcription of the RD29ACitation
Mahfouz MM, Li L, Piatek M, Fang X, Mansour H, et al. (2011) Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein. Plant Molecular Biology 78: 311-321. doi:10.1007/s11103-011-9866-x.Publisher
Springer NatureJournal
Plant Molecular BiologyPubMed ID
22167390PubMed Central ID
PMC3259320Scopus Count
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