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dc.contributor.authorZimaro, Tamara
dc.contributor.authorThomas, Ludivine
dc.contributor.authorMarondedze, Claudius
dc.contributor.authorGaravaglia, Betiana S
dc.contributor.authorGehring, Christoph A
dc.contributor.authorOttado, Jorgelina
dc.contributor.authorGottig, Natalia
dc.date.accessioned2014-08-27T09:42:12Z
dc.date.available2014-08-27T09:42:12Z
dc.date.issued2013-08-07
dc.identifier.citationZimaro T, Thomas L, Marondedze C, Garavaglia BS, Gehring C, et al. (2013) Insights into xanthomonas axonopodis pv. citri biofilm through proteomics. BMC Microbiology 13: 186. doi:10.1186/1471-2180-13-186.
dc.identifier.issn14712180
dc.identifier.pmid23924281
dc.identifier.doi10.1186/1471-2180-13-186
dc.identifier.urihttp://hdl.handle.net/10754/325246
dc.description.abstractBackground: Xanthomonas axonopodis pv. Citri (X. a. pv. Citri) causes citrus canker that can result in defoliation and premature fruit drop with significant production losses worldwide. Biofilm formation is an important process in bacterial pathogens and several lines of evidence suggest that in X. a. pv. Citri this process is a requirement to achieve maximal virulence since it has a major role in host interactions. In this study, proteomics was used to gain further insights into the functions of biofilms. Results: In order to identify differentially expressed proteins, a comparative proteomic study using 2D difference gel electrophoresis was carried out on X. a. pv. Citri mature biofilm and planktonic cells. The biofilm proteome showed major variations in the composition of outer membrane proteins and receptor or transport proteins. Among them, several porins and TonB-dependent receptor were differentially regulated in the biofilm compared to the planktonic cells, indicating that these proteins may serve in maintaining specific membrane-associated functions including signaling and cellular homeostasis. In biofilms, UDP-glucose dehydrogenase with a major role in exopolysaccharide production and the non-fimbrial adhesin YapH involved in adherence were over-expressed, while a polynucleotide phosphorylase that was demonstrated to negatively control biofilm formation in E. coli was down-regulated. In addition, several proteins involved in protein synthesis, folding and stabilization were up-regulated in biofilms. Interestingly, some proteins related to energy production, such as ATP-synthase were down-regulated in biofilms. Moreover, a number of enzymes of the tricarboxylic acid cycle were differentially expressed. In addition, X. a. pv. Citri biofilms also showed down-regulation of several antioxidant enzymes. The respective gene expression patterns of several identified proteins in both X. a. pv. Citri mature biofilm and planktonic cells were evaluated by quantitative real-time PCR and shown to consistently correlate with those deduced from the proteomic study. Conclusions: Differentially expressed proteins are enriched in functional categories. Firstly, proteins that are down-regulated in X. a. pv. Citri biofilms are enriched for the gene ontology (GO) terms 'generation of precursor metabolites and energy' and secondly, the biofilm proteome mainly changes in 'outer membrane and receptor or transport'. We argue that the differentially expressed proteins have a critical role in maintaining a functional external structure as well as enabling appropriate flow of nutrients and signals specific to the biofilm lifestyle. 2013 Zimaro et al.; licensee BioMed Central Ltd.
dc.language.isoen
dc.publisherSpringer Nature
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.subjectBiofilm
dc.subjectCitrus canker
dc.subjectProteomics
dc.subjectXanthomonas axonopodis pv. Citri
dc.subjectcarrier protein
dc.subjectexopolysaccharide
dc.subjectpolyribonucleotide nucleotidyltransferase
dc.subjectporin
dc.subjectprotein TonB
dc.subjectproton transporting adenosine triphosphate synthase
dc.subjectreceptor
dc.subjecturidine diphosphate glucose dehydrogenase
dc.subjectbacterium adherence
dc.subjectbiofilm
dc.subjectcitric acid cycle
dc.subjectcontrolled study
dc.subjectdown regulation
dc.subjectenergy yield
dc.subjectgel electrophoresis
dc.subjectgene expression
dc.subjectplankton
dc.subjectprotein folding
dc.subjectprotein synthesis
dc.subjectproteomics
dc.subjectquantitative analysis
dc.subjectreal time polymerase chain reaction
dc.subjectregulatory mechanism
dc.subjectsignal transduction
dc.subjectupregulation
dc.subjectXanthomonas citri
dc.subjectBacterial Proteins
dc.subjectBiofilms
dc.subjectCitrus
dc.subjectGene Expression Regulation, Bacterial
dc.subjectPlant Diseases
dc.subjectXanthomonas axonopodis
dc.subjectBacteria (microorganisms)
dc.subjectCitrus
dc.subjectXanthomonas axonopodis pv. citri
dc.titleInsights into xanthomonas axonopodis pv. Citri biofilm through proteomics
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Core Lab
dc.contributor.departmentBioscience Program
dc.contributor.departmentMolecular Signalling Group
dc.identifier.journalBMC Microbiology
dc.identifier.pmcidPMC3750573
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstituto de Biologa Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Cientficas y Tcnicas , Ocampo y Esmeralda, Rosario, Santa Fe, Argentina
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personThomas, Ludivine
kaust.personMarondedze, Claudius
kaust.personGehring, Christoph A.
refterms.dateFOA2018-06-14T03:46:25Z
dc.date.published-online2013-08-07
dc.date.published-print2013


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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.