Recent Submissions

  • Natal philopatry increases relatedness within groups of coral reef cardinalfish

    Rueger, Theresa; Harrison, Hugo B.; Buston, Peter M.; Gardiner, Naomi M.; Berumen, Michael L.; Jones, G. P. (Proceedings of the Royal Society B: Biological Sciences, The Royal Society, 2020-07-08) [Article]
    A central issue in evolutionary ecology is how patterns of dispersal influence patterns of relatedness in populations. In terrestrial organisms, limited dispersal of offspring leads to groups of related individuals. By contrast, for most marine organisms, larval dispersal in open waters is thought to minimize kin associations within populations. However, recent molecular evidence and theoretical approaches have shown that limited dispersal, sibling cohesion and/or differential reproductive success can lead to kin association and elevated relatedness. Here, we tested the hypothesis that limited dispersal explains small-scale patterns of relatedness in the pajama cardinalfish Sphaeramia nematoptera. We used 19 microsatellite markers to assess parentage of 233 juveniles and pairwise relatedness among 527 individuals from 41 groups in Kimbe Bay, Papua New Guinea. Our findings support three predictions of the limited dispersal hypothesis: (i) elevated relatedness within groups, compared with among groups and elevated relatedness within reefs compared with among reefs; (ii) a weak negative correlation of relatedness with distance; (iii) more juveniles than would be expected by chance in the same group and the same reef as their parents. We provide the first example for natal philopatry at the group level causing small-scale patterns of genetic relatedness in a marine fish.
  • Chemoselective Hydrogenation of Alkynes to (Z)-Alkenes Using an Air-Stable Base Metal Catalyst

    Zubar, Viktoriia; Sklyaruk, Jan; Brzozowska, Aleksandra; Rueping, Magnus (Organic Letters, American Chemical Society (ACS), 2020-07-08) [Article]
    A highly selective hydrogenation of alkynes using an air-stable and readily available manganese catalyst has been achieved. The reaction proceeds under mild reaction conditions and tolerates various functional groups, resulting in (Z)-alkenes and allylic alcohols in high yields. Mechanistic experiments suggest that the reaction proceeds via a bifunctional activation involving metal–ligand cooperativity.
  • Seagrass losses since mid-20th century fuelled CO 2 emissions from soil carbon stocks

    Salinas, Cristian; Duarte, Carlos M.; Lavery, P. S.; Masqué, Pere; Arias-Ortiz, Ariane; Leon, Javier X.; Callaghan, David; Kendrick, G. A.; Serrano, Oscar (Global Change Biology, Wiley, 2020-07-07) [Article]
    Seagrass meadows store globally significant organic carbon (Corg) stocks which, if disturbed, can lead to CO2 emissions, contributing to climate change. Eutrophication and thermal stress continue to be a major cause of seagrass decline worldwide, but the associated CO2 emissions remain poorly understood. This study presents comprehensive estimates of seagrass soil Corg erosion following eutrophication-driven seagrass loss in Cockburn Sound (23 km2 between 1960s and 1990s) and identifies the main drivers. We estimate that shallow seagrass meadows (<5 m depth) had significantly higher Corg stocks in 50 cm thick soils (4.5 ± 0.7 kg Corg/m2) than previously vegetated counterparts (0.5 ± 0.1 kg Corg/m2). In deeper areas (>5 m), however, soil Corg stocks in seagrass and bare but previously vegetated areas were not significantly different (2.6 ± 0.3 and 3.0 ± 0.6 kg Corg/m2, respectively). The soil Corg sequestration capacity prevailed in shallow and deep vegetated areas (55 ± 11 and 21 ± 7 g Corg m−2 year−1, respectively), but was lost in bare areas. We identified that seagrass canopy loss alone does not necessarily drive changes in soil Corg but, when combined with high hydrodynamic energy, significant erosion occurred. Our estimates point at ~0.20 m/s as the critical shear velocity threshold causing soil Corg erosion. We estimate, from field studies and satellite imagery, that soil Corg erosion (within the top 50 cm) following seagrass loss likely resulted in cumulative emissions of 0.06–0.14 Tg CO2-eq over the last 40 years in Cockburn Sound. We estimated that indirect impacts (i.e. eutrophication, thermal stress and light stress) causing the loss of ~161,150 ha of seagrasses in Australia, likely resulted in the release of 11–21 Tg CO2-eq since the 1950s, increasing cumulative CO2 emissions from land-use change in Australia by 1.1%–2.3% per annum. The patterns described serve as a baseline to estimate potential CO2 emissions following disturbance of seagrass meadows.
  • SARS-CoV-2 infections and COVID-19 mortalities strongly correlate with ACE1 I/D genotype.

    Yamamoto, Naoki; Ariumi, Yasuo; Nishida, Nao; Yamamoto, Rain; Bauer, Georg; Gojobori, Takashi; Shimotohno, Kunitada; Mizokami, Masashi (Gene, Elsevier BV, 2020-07-07) [Article]
    Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19). The relentless spread and pathogenicity of the virus have become a global public health emergency. One of the striking features of this pandemic is the pronounced impact on specific regions and ethnic groups. In particular, compared with East Asia, where the virus first emerged, SARS-CoV-2 has caused high rates of morbidity and mortality in Europe. This has not been experienced in past global viral infections, such as influenza, severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) and is unique to SARS-CoV-2. For this reason, we investigated the involvement of genetic factors associated with SARS-CoV-2 infection with a focus on angiotensin-converting enzyme (ACE)-related genes, because ACE2 is a receptor for SARS-CoV-2. We found that the ACE1 II genotype frequency in a population was significantly negatively correlated with the number of SARS-CoV-2 cases. Similarly, the ACE1 II genotype was negatively correlated with the number of deaths due to SARS-CoV-2 infection. These data suggest that the ACE1 II genotype may influence the prevalence and clinical outcome of COVID-19 and serve as a predictive marker for COVID-19 risk and severity.
  • Semiparametric estimation of cross-covariance functions for multivariate random fields

    Qadir, Ghulam A.; Sun, Ying (Biometrics, Wiley, 2020-07-06) [Article]
    The prevalence of spatially referenced multivariate data has impelled researchers to develop procedures for joint modeling of multiple spatial processes. This ordinarily involves modeling marginal and cross-process dependence for any arbitrary pair of locations using a multivariate spatial covariance function. However, building a flexible multivariate spatial covariance function that is nonnegative definite is challenging. Here, we propose a semiparametric approach for multivariate spatial covariance function estimation with approximate Matérn marginals and highly flexible cross-covariance functions via their spectral representations. The flexibility in our cross-covariance function arises due to B-spline based specification of the underlying coherence functions, which in turn allows us to capture non-trivial cross-spectral features. We then develop a likelihood-based estimation procedure and perform multiple simulation studies to demonstrate the performance of our method, especially on the coherence function estimation. Finally, we analyze particulate matter concentrations (PM2.5) and wind speed data over the West-North-Central climatic region of the United States, where we illustrate that our proposed method outperforms the commonly used full bivariate Matérn model and the linear model of coregionalization for spatial prediction.
  • Expression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco.

    Moreno, Juan C; Mi, Jianing; Agrawal, Shreya; Kössler, Stella; Turečková, Veronika; Tarkowská, Danuše; Thiele, Wolfram; Al-Babili, Salim; Bock, Ralph; Schöttler, Mark Aurel (The Plant journal : for cell and molecular biology, Wiley, 2020-07-06) [Article]
    Because carotenoids act as accessory pigments in photosynthesis, play a key photoprotective role, and are of major nutritional importance, carotenogenesis has been a target for crop improvement. Although carotenoids are important precursors of phytohormones, previous genetic manipulations reported little if any effects on biomass production and plant development, but resulted in specific modifications in carotenoid content. Unexpectedly, the expression of the carrot lycopene b-cyclase (DcLCYB1) in Nicotiana tabacum cv. Xanthi not only resulted in increased carotenoid accumulation, but also in altered plant architecture characterized by longer internodes, faster plant growth, early flowering and increased biomass. Here, we have challenged these transformants with a range of growth conditions to determine the robustness of their phenotype and analyze the underlying mechanisms. Transgenic DcLCYB1 lines showed increased transcript levels of key genes involved in carotenoid, chlorophyll, gibberellin (GA) and abscisic acid (ABA) biosynthesis, but also in photosynthesis-related genes. Accordingly, their carotenoid, chlorophyll, ABA and GA contents were increased. Hormone application and inhibitor experiments confirmed the key role of altered GA/ABA contents in the growth phenotype. Because the longer internodes reduce shading of mature leaves, induction of leaf senescence was delayed, and mature leaves maintained a high photosynthetic capacity. This increased total plant assimilation, as reflected in higher plant yields under both fully-controlled constant and fluctuating light, and in non-controlled conditions. Furthermore, our data is a warning that engineering of isoprenoid metabolism can cause complex changes in phytohormone homeostasis and therefore plant development, which have not been sufficiently considered in previous studies.
  • Unfamiliar partnerships limit cnidarian holobiont acclimation to warming

    Herrera Sarrias, Marcela; Klein, Shannon; Schmidt-Roach, Sebastian; Campana, Sara; Cziesielski, Maha Joana; Chen, Jit Ern; Duarte, Carlos M.; Aranda, Manuel (Global Change Biology, Wiley, 2020-07-06) [Article]
    Enhancing the resilience of corals to rising temperatures is now a matter of urgency, leading to growing efforts to explore the use of heat tolerant symbiont species to improve their thermal resilience. The notion that adaptive traits can be retained by transferring the symbionts alone, however, challenges the holobiont concept, a fundamental paradigm in coral research. Holobiont traits are products of a specific community (holobiont) and all its co-evolutionary and local adaptations, which might limit the retention or transference of holobiont traits by exchanging only one partner. Here, we evaluate how interchanging partners affect the short- and long-term performance of holobionts under heat stress using clonal lineages of the cnidarian model system Aiptasia (host and Symbiodiniaceae strains) originating from distinct thermal environments. Our results show that holobionts from more thermally variable environments have higher plasticity to heat stress, but this resilience could not be transferred to other host genotypes through the exchange of symbionts. Importantly, our findings highlight the role of the host in determining holobiont productivity in response to thermal stress and indicate that local adaptations of holobionts will likely limit the efficacy of interchanging unfamiliar compartments to enhance thermal tolerance.
  • Numerical Study of CH4 Generation and Transport in XLPE-Insulated Cables in Continuous Vulcanization

    Ruslan, Mohd Fuad Anwari Che; Youn, Dong Joon; Aarons, Roshan; Sun, Yabin; Sun, Shuyu (Materials, MDPI AG, 2020-07-06) [Article]
    <jats:p>In this work, we apply a computational diffusion model based on Fick’s laws to study the generation and transport of methane (CH 4 ) during the production of a cross-linked polyethylene (XLPE) insulated cable. The model takes into account the heating process in a curing tube where most of the cross-linking reaction occurs and the subsequent two-stage cooling process, with water and air as the cooling media. For the calculation of CH 4 generation, the model considers the effect of temperature on the cross-linking reaction selectivity. The cross-linking reaction selectivity is a measure of the preference of cumyloxy to proceed either with a hydrogen abstraction reaction, which produces cumyl alcohol, or with a β -scission reaction, which produces acetophenone and CH 4 . The simulation results show that, during cable production, a significant amount of CH 4 is generated in the XLPE layer, which diffuses out of the cable and into the conductor part of the cable. Therefore, the diffusion pattern becomes a non-uniform radial distribution of CH 4 at the cable take-up point, which corresponds well with existing experimental data. Using the model, we perform a series of parametric studies to determine the effect of the cable production conditions, such as the curing temperature, line speed, and cooling water flow rate, on CH 4 generation and transport during cable production. The results show that the curing temperature has the largest impact on the amount of CH 4 generated and its distribution within the cable. We found that under similar curing and cooling conditions, varying the line speed induces a notable effect on the CH 4 transport within the cable, while the cooling water flow rate had no significant impact.</jats:p>
  • Solution-processable and photopolymerisable TiO2 nanorods as dielectric layers for thin film transistors

    Cheng, Fei; Verrelli, Emanuele; Alharthi, Fahad A.; Das, Satyajit; Anthopoulos, Thomas D.; Lai, Khue T.; Kemp, Neil T.; O'Neill, Mary; Kelly, Stephen M. (RSC Advances, Royal Society of Chemistry (RSC), 2020-07-06) [Article]
    <p>A prototype solution-processed n-type thin film transistor was fabricated. The film incorporates a dielectric layer prepared from solution-processed and photopolymerised inorganic/organic TiO$_{2}$ nanorods and zinc oxide as the semiconductor, also deposited from solution.</p>
  • Biofouling control by phosphorus limitation strongly depends on the assimilable organic carbon concentration.

    Javier, Luisa; Farhat, Nadia M; Desmond, Peter; Linares, Rodrigo Valladares; Bucs,Szilard; Kruithof, Joop C; Vrouwenvelder, Johannes S. (Water research, Elsevier BV, 2020-07-05) [Article]
    Nutrient limitation is a biofouling control strategy in reverse osmosis (RO) membrane systems. In seawater, the assimilable organic carbon content available for bacterial growth ranges from about 50 to 400 μg C·L-1, while the phosphorus concentration ranges from 3 to 11 μg P·L-1. Several studies monitored biofouling development, limiting either carbon or phosphorus. The effect of carbon to phosphorus ratio and the restriction of both nutrients on membrane system performance have not yet been investigated. This study examines the impact of reduced phosphorus concentration (from 25 μg P·L-1 and 3 μg P·L-1, to a low concentration of ≤0.3 μg P·L-1), combined with two different carbon concentrations (250 C L-1 and 30 μg C·L-1), on biofilm development in an RO system. Feed channel pressure drop was measured to determine the effect of the developed biofilm on system performance. The morphology of the accumulated biomass for both carbon concentrations was characterized by optical coherence tomography (OCT) and the biomass amount and composition was quantified by measuring total organic carbon (TOC), adenosine triphosphate (ATP), total cell counts (TCC), and extracellular polymeric substances (EPS) concentration for the developed biofilms under phosphorus restricted (P-restricted) and dosed (P-dosed) conditions. For both carbon concentrations, P-restricted conditions (≤0.3 μg P·L-1) limited bacterial growth (lower values of ATP, TCC). A faster pressure drop increase was observed for P-restricted conditions compared to P-dosed conditions when 250 μg C·L-1 was dosed. This faster pressure drop increase can be explained by a higher area covered by biofilm in the flow channel and a higher amount of produced EPS. Conversely, a slower pressure drop increase was observed for P-restricted conditions compared to P-dosed conditions when 30 μg C·L-1 was dosed. Results of this study demonstrate that P-limitation delayed biofilm formation effectively when combined with low assimilable organic carbon concentration and thereby, lengthening the overall membrane system performance.
  • Effect of organic micropollutants on biofouling in a forward osmosis process integrating seawater desalination and wastewater reclamation

    Kim,Youngjin; Kim, Lan Hee; Vrouwenvelder, Johannes S.; Ghaffour, NorEddine (Journal of Hazardous Materials, Elsevier BV, 2020-07-04) [Article]
    This study systematically investigated the effect of organic micropollutants (OMPs) on biofouling in forward osmosis (FO) integrating wastewater treatment and seawater dilution. Synthetic seawater (0.6 M sodium chloride) was used as a draw solution and synthetic municipal wastewater as a feed solution. To evaluate the impact of OMPs in a replicate parallel study, wastewater was supplemented with a mixture of 7 OMPs (OMPs-feed) and without OMPs (control) during 8 batch filtration cycles with feed and draw solution replacement after each filtration. The FO performance (water flux), development and microbial composition properties of biofilm layers on the wastewater side of the FO membrane were studied. Compared to the control without OMPs, the FO fed with OMPs containing wastewater showed (i) initially the same water flux and flux decline during the first filtration cycle, (ii) with increasing filtration cycle a lower flux decline and (iii) lower concentrations for the total cells, ATP, EPS carbohydrates and proteins in biofilm layers, and (iv) a lower diversity of the biofilm microbial community composition (indicating selective pressure) and (v) increasing rejection of 6 of the 7 OMPs. In essence, biofouling on the FO membrane showed (i) a lower flux decline in the presence of OMPs in the feed water and (ii) a higher OMPs rejection, both illustrating better membrane performance. This study has a significant implication for optimizing osmotic dilution in terms of FO operation and OMPs rejection.
  • Autoignition of diethyl ether and a diethyl ether/ethanol blend

    Issayev, Gani; Sarathy, Mani; Farooq, Aamir (Fuel, Elsevier BV, 2020-07-04) [Article]
    Binary blends of fast-reacting diethyl ether (DEE) and slow-reacting ethanol (EtOH) are quite promising as renewable replacements for conventional fuels in modern compression ignition engines. In this work, pure diethyl ether and a 50/50 M binary blend of diethyl ether and ethanol (DEE/EtOH) were investigated in a shock tube and a rapid compression machine. Ignition delay times were measured over the temperature range of 550–1000 K, pressures of 20–40 bar, and equivalence ratios of 0.5–1. Literature reaction mechanisms of diethyl ether and ethanol were combined to simulate the reactivity trends of the blends. Species rate-of-production and sensitivity analyses were performed to analyze the interplay between radicals originating from the two fuels. Multistage ignition behavior was observed in both experiments and simulations, with peculiar 3-stage ignition visible at fuel-lean conditions. Kinetic analyses were used to identify the reactions controlling various stages of ignition. Reactivity comparison of DEE/EtOH and dimethyl ether/ethanol (DME/EtOH) blends showed that the oxidation of DEE blends is controlled by acetaldehyde whereas formaldehyde controls the oxidation of DME blends.
  • Unrealistic energy and materials requirement for direct air capture in deep mitigation pathways

    Chatterjee, Sudipta; Huang, Kuo-Wei (Nature Communications, Springer Science and Business Media LLC, 2020-07-03) [Article]
    The increasing global atmospheric CO2 concentration due to heavy reliance on fossil fuels as the primary energy sources (~410 ppm in 2019)1 has made direct extraction or removal of CO2 from ambient air (direct air carbon capture (DACC)) the most logical alternative over traditional modes of carbon capture from large stationary sources because of many of the perceived advantages and compelling arguments2. With the current level of CO2 emissions (32.6 gigatons (Gt)-CO2/year2017)1, Realmonte and co-workers recently imposed the global capacity at 30 Gt-CO2/year as a case study for DACC, and concluded that “in theory DACCS can be an enabling factor for the Paris Agreement objectives” and recommended the policy makers to “support an acceleration in development and deployment of DACCS”3. While challenges of large-scale CO2 utilization and sequestration were recognized and these approaches were deemed impractical4,5, our analysis further showed that the energy and materials requirements for DACC are unrealistic even when the most promising technologies are employed. Thus, DACC is unfortunately only an energetically and financially costly distraction in effective mitigation of climate changes at a meaningful scale before we achieve the status of a significant surplus of carbon-neutral/low-carbon energy.
  • Bioconversion of swine manure into high-value products of medium chain fatty acids.

    Zhang, Wanqin; Yin, Fubin; Dong, Hongmin; Cao, Qitao; Wang, Shunli; Xu, Jiajie; Zhu, Zhiping (Waste management (New York, N.Y.), Elsevier BV, 2020-07-03) [Article]
    This research proposes and demonstrates, for the first time, the utilization of swine manure as a complex feedstock to produce high-value medium chain fatty acids (MCFA). The two-stage anaerobic digestion (AD) carboxylates platform was adopted for the conversion of swine manure to short chain fatty acids (SCFAs) and then SCFAs to MCFA (n-caproate, n-heptanoate, and n-caprylate) with ethanol supplementation. We defined the appropriate initial pH of 10.0 for SCFAs production with a carbon conversion rate of 71.2%, and acetate, propionate were the main products, which accounted for around 72.9% of the total SCFAs in the primary stage (I). Through the addition of ethanol, 61.3% of the converted carbon in the complex SCFAs solution was converted into MCFA (C6-C8) in the chain elongation stage (II), while only 6.7% was attributed to methane formation. The concentrations of n-caproate, n-heptanoate, and n-caprylate reached 8.6 g COD/L (3.9 g/L), 6.4 g COD/L (2.7 g/L), and 2.6 g COD/L (1.07 g/L), respectively. This study achieved a relatively higher concentration of n-heptanoate compared with past studies of MCFA from other feedstock. These findings demonstrated a new route for resource recovery and the operating parameters for producing MCFA from swine manure.
  • A Highly Conductive Titanium Oxynitride Electron-Selective Contact for Efficient Photovoltaic Devices.

    Yang, Xinbo; Lin, Yuanbao; Liu, Jiang; Liu, Wenzhu; Bi, Qunyu; Song, Xin; Kang, Jingxuan; Xu, Fuzong; Xu, Lujia; Hedhili, Mohamed N.; Baran, Derya; Zhang, Xiaohong; Anthopoulos, Thomas D.; De Wolf, Stefaan (Advanced materials (Deerfield Beach, Fla.), Wiley, 2020-07-03) [Article]
    High-quality carrier-selective contacts with suitable electronic properties are a prerequisite for photovoltaic devices with high power conversion efficiency (PCE). In this work, an efficient electron-selective contact, titanium oxynitride (TiOx Ny ), is developed for crystalline silicon (c-Si) and organic photovoltaic devices. Atomic-layer-deposited TiOx Ny is demonstrated to be highly conductive with a proper work function (4.3 eV) and a wide bandgap (3.4 eV). Thin TiOx Ny films simultaneously provide a moderate surface passivation and enable a low contact resistivity on c-Si surfaces. By implementation of an optimal TiOx Ny -based contact, a state-of-the-art PCE of 22.3% is achieved for a c-Si solar cell featuring a full-area dopant-free electron-selective contact. Simultaneously, conductive TiOx Ny is proven to be an efficient electron-transport layer for organic photovoltaic (OPV) devices. A remarkably high PCE of 17.02% is achieved for an OPV device with an electron-transport TiOx Ny layer, which is superior to conventional ZnO-based devices with a PCE of 16.10%. Atomic-layer-deposited TiOx Ny ETL on a large area with a high uniformity may help accelerate the commercialization of emerging solar technologies.
  • Temperature-resilient solid-state organic artificial synapses for neuromorphic computing

    Melianas, Armantas; Quill, T. J.; LeCroy, G.; Tuchman, Y.; Loo, H. v.; Keene, S. T.; Giovannitti, Alexander; Lee, H. R.; Maria, I. P.; McCulloch, Iain; Salleo, Alberto (Science Advances, American Association for the Advancement of Science (AAAS), 2020-07-03) [Article]
    <jats:p>Devices with tunable resistance are highly sought after for neuromorphic computing. Conventional resistive memories, however, suffer from nonlinear and asymmetric resistance tuning and excessive write noise, degrading artificial neural network (ANN) accelerator performance. Emerging electrochemical random-access memories (ECRAMs) display write linearity, which enables substantially faster ANN training by array programing in parallel. However, state-of-the-art ECRAMs have not yet demonstrated stable and efficient operation at temperatures required for packaged electronic devices (~90°C). Here, we show that (semi)conducting polymers combined with ion gel electrolyte films enable solid-state ECRAMs with stable and nearly temperature-independent operation up to 90°C. These ECRAMs show linear resistance tuning over a >2× dynamic range, 20-nanosecond switching, submicrosecond write-read cycling, low noise, and low-voltage (±1 volt) and low-energy (~80 femtojoules per write) operation combined with excellent endurance (>10$^\{9}$$ write-read operations at 90°C). Demonstration of these high-performance ECRAMs is a fundamental step toward their implementation in hardware ANNs.</jats:p>
  • Deracemization and Stereoinversion of Alcohols Using Two Mutants of Secondary Alcohol Dehydrogenase from Thermoanaerobacter pseudoethanolicus

    Nafiu, Sodiq A.; Takahashi, Masateru; Takahashi, Etsuko; Hamdan, Samir; Musa, Musa M. (European Journal of Organic Chemistry, Wiley, 2020-07-03) [Article]
    We developed a one-pot two-step deracemization approach for alcohols using two mutants of Thermoanaerobacter pseudoethanolicus secondary alcohol dehydrogenase ( Te SADH). This approach relies on consecutive non-stereospecific oxidation of alcohols and stereoselective reduction of their prochiral ketones using two mutants of Te SADH with poor and good stereoselectivities, respectively. More specifically, W110G Te SADH enables a non-stereospecific oxidation of alcohol racemates to their corresponding prochiral ketones, followed by W110V Te SADH-catalyzed stereoselective reduction of the resultant ketone intermediates to enantiopure ( S )-configured alcohols in up to >99% enantiomeric excess. A heat treatment after the oxidation step was required to avoid the interference of the marginally stereoselective W110G Te SADH in the reduction step; this heat treatment was eliminated by using sol-gel encapsulated W110G Te SADH in the oxidation step. Moreover, this bi-enzymatic approach was implemented in the stereoinversion of ( R )-configured alcohols, and ( S )-configured alcohols with up to >99% enantiomeric excess were obtained by this Mitsunobu-like stereoinversion reaction.
  • Transmissivity and Reflectivity of a TE-polarized Wave Incident on a Microcavity Containing Strongly Coupled Excitons with In-plane Uniaxially Oriented Transition Dipole Moments

    Le Roux, Florian; Anthony Taylor, Robert; Bradley, Donal Donat Conor (physica status solidi (b), Wiley, 2020-07-03) [Article]
    This work examines the reflectivity and transmissivity of a TE-polarized wave incident on a microcavity containing strongly coupled excitons with in-plane uniaxially oriented transition dipole moments and presents a different interpretation to a previous report. The propagation of the electric field inside the cavity is discussed and a distinction is made between two different physical cases: the first, previously observed, and the second, which enables the interpretation of measurements performed on a microcavity containing an oriented layer of liquid-crystalline poly(9,9-dioctylfluorene). In all cases, the reflected and transmitted electric fields derive from photons leaking parallel and perpendicular to the transition dipole moment orientation.
  • Early-Stage Growth Mechanism and Synthesis Conditions-Dependent Morphology of Nanocrystalline Bi Films Electrodeposited from Perchlorate Electrolyte.

    Tishkevich, Daria; Grabchikov, Sergey; Zubar, Tatiana; Vasin, Denis; Trukhanov, Sergei; Vorobjova, Alla; Yakimchuk, Dmitry; Kozlovskiy, Artem; Zdorovets, Maxim; Giniyatova, Sholpan; Shimanovich, Dmitriy; Lyakhov, Dmitry; Michels, Dominik L.; Dong, Mengge; Gudkova, Svetlana; Trukhanov, Alex (Nanomaterials (Basel, Switzerland), MDPI AG, 2020-07-02) [Article]
    Bi nanocrystalline films were formed from perchlorate electrolyte (PE) on Cu substrate via electrochemical deposition with different duration and current densities. The microstructural, morphological properties, and elemental composition were studied using scanning electron microscopy (SEM), atomic force microscopy (AFM), and energy-dispersive X-ray microanalysis (EDX). The optimal range of current densities for Bi electrodeposition in PE using polarization measurements was demonstrated. For the first time, it was shown and explained why, with a deposition duration of 1 s, co-deposition of Pb and Bi occurs. The correlation between synthesis conditions and chemical composition and microstructure for Bi films was discussed. The analysis of the microstructure evolution revealed the changing mechanism of the films' growth from pillar-like (for Pb-rich phase) to layered granular form (for Bi) with deposition duration rising. This abnormal behavior is explained by the appearance of a strong Bi growth texture and coalescence effects. The investigations of porosity showed that Bi films have a closely-packed microstructure. The main stages and the growth mechanism of Bi films in the galvanostatic regime in PE with a deposition duration of 1-30 s are proposed.
  • Alternative splicing and allosteric regulation modulate the chromatin binding of UHRF1.

    Tauber, Maria; Kreuz, Sarah; Lemak, Alexander; Mandal, Papita; Yerkesh, Zhadyra; Veluchamy, Alaguraj; Al-Gashgari, Bothayna; Aljahani, Abrar; Cortés-Medina, Lorena V; Azhibek, Dulat; Fan, Lixin; Ong, Michelle S; Duan, Shili; Houliston, Scott; Arrowsmith, Cheryl H; Fischle, Wolfgang (Nucleic acids research, Oxford University Press (OUP), 2020-07-02) [Article]
    UHRF1 is an important epigenetic regulator associated with apoptosis and tumour development. It is a multidomain protein that integrates readout of different histone modification states and DNA methylation with enzymatic histone ubiquitylation activity. Emerging evidence indicates that the chromatin-binding and enzymatic modules of UHRF1 do not act in isolation but interplay in a coordinated and regulated manner. Here, we compared two splicing variants (V1, V2) of murine UHRF1 (mUHRF1) with human UHRF1 (hUHRF1). We show that insertion of nine amino acids in a linker region connecting the different TTD and PHD histone modification-binding domains causes distinct H3K9me3-binding behaviour of mUHRF1 V1. Structural analysis suggests that in mUHRF1 V1, in contrast to V2 and hUHRF1, the linker is anchored in a surface groove of the TTD domain, resulting in creation of a coupled TTD-PHD module. This establishes multivalent, synergistic H3-tail binding causing distinct cellular localization and enhanced H3K9me3-nucleosome ubiquitylation activity. In contrast to hUHRF1, H3K9me3-binding of the murine proteins is not allosterically regulated by phosphatidylinositol 5-phosphate that interacts with a separate less-conserved polybasic linker region of the protein. Our results highlight the importance of flexible linkers in regulating multidomain chromatin binding proteins and point to divergent evolution of their regulation.

View more