Unique prokaryotic consortia in geochemically distinct sediments from Red Sea Atlantis II and discovery deep brine pools.
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Figure S1 - Taxonomic assignment and relative abundance of bacterial OTUs in ATII, DD, CD and BI sediment samples.
Figure S2 - Heat map and dendrogram of low-abundance bacterial groups based on recovery of 16S rDNA tags.
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Table S1 - The archaeal assigned OTUs (from Fig. 3A) and the bacterial assigned OTUs (from Fig. 3B) are tabulated.
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Table S2 - The rare bacterial assigned OTUs (from SF2) are tabulated.
Mustafa, Ghada A
Ramadan, Adham R
Bajic, Vladimir B.
Marsis, Nardine G R
Coolen, Marco J L
Ferreira, Ari J S
Dorry, Hamza El
KAUST DepartmentRed Sea Research Center (RSRC)
Computational Bioscience Research Center (CBRC)
Applied Mathematics and Computational Science Program
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Marine Science Program
Biological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/323524
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AbstractThe seafloor is a unique environment, which allows insights into how geochemical processes affect the diversity of biological life. Among its diverse ecosystems are deep-sea brine pools - water bodies characterized by a unique combination of extreme conditions. The 'polyextremophiles' that constitute the microbial assemblage of these deep hot brines have not been comprehensively studied. We report a comparative taxonomic analysis of the prokaryotic communities of the sediments directly below the Red Sea brine pools, namely, Atlantis II, Discovery, Chain Deep, and an adjacent brine-influenced site. Analyses of sediment samples and high-throughput pyrosequencing of PCR-amplified environmental 16S ribosomal RNA genes (16S rDNA) revealed that one sulfur (S)-rich Atlantis II and one nitrogen (N)-rich Discovery Deep section contained distinct microbial populations that differed from those found in the other sediment samples examined. Proteobacteria, Actinobacteria, Cyanobacteria, Deferribacteres, and Euryarchaeota were the most abundant bacterial and archaeal phyla in both the S- and N-rich sections. Relative abundance-based hierarchical clustering of the 16S rDNA pyrotags assigned to major taxonomic groups allowed us to categorize the archaeal and bacterial communities into three major and distinct groups; group I was unique to the S-rich Atlantis II section (ATII-1), group II was characteristic for the N-rich Discovery sample (DD-1), and group III reflected the composition of the remaining sediments. Many of the groups detected in the S-rich Atlantis II section are likely to play a dominant role in the cycling of methane and sulfur due to their phylogenetic affiliations with bacteria and archaea involved in anaerobic methane oxidation and sulfate reduction.
CitationSiam R, Mustafa GA, Sharaf H, Moustafa A, Ramadan AR, et al. (2012) Unique Prokaryotic Consortia in Geochemically Distinct Sediments from Red Sea Atlantis II and Discovery Deep Brine Pools. PLoS ONE 7: e42872. doi:10.1371/journal.pone.0042872.
PublisherPublic Library of Science (PLoS)
PubMed Central IDPMC3423430
CollectionsArticles; Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC); Marine Science Program; Applied Mathematics and Computational Science Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
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