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dc.contributor.authorJessen, Christian
dc.contributor.authorLizcano, Javier
dc.contributor.authorBayer, Till
dc.contributor.authorRoder, Cornelia
dc.contributor.authorAranda, Manuel
dc.contributor.authorWild, Christian
dc.contributor.authorVoolstra, Christian R.
dc.date.accessioned2014-07-21T13:17:41Z
dc.date.available2014-07-21T13:17:41Z
dc.date.issued2013-04-22
dc.identifier.citationJessen C, Villa Lizcano JF, Bayer T, Roder C, Aranda M, et al. (2013) In-situ Effects of Eutrophication and Overfishing on Physiology and Bacterial Diversity of the Red Sea Coral Acropora hemprichii. PLoS ONE 8: e62091. doi:10.1371/journal.pone.0062091.
dc.identifier.issn1932-6203
dc.identifier.pmid23630625
dc.identifier.doi10.1371/journal.pone.0062091
dc.identifier.urihttp://hdl.handle.net/10754/323520
dc.description.abstractCoral reefs of the Central Red Sea display a high degree of endemism, and are increasingly threatened by anthropogenic effects due to intense local coastal development measures. Overfishing and eutrophication are among the most significant local pressures on these reefs, but there is no information available about their potential effects on the associated microbial community. Therefore, we compared holobiont physiology and 16S-based bacterial communities of tissue and mucus of the hard coral Acropora hemprichii after 1 and 16 weeks of in-situ inorganic nutrient enrichment (via fertilizer diffusion) and/or herbivore exclusion (via caging) in an offshore reef of the Central Red Sea. Simulated eutrophication and/or overfishing treatments did not affect coral physiology with respect to coral respiration rates, chlorophyll a content, zooxanthellae abundance, or δ (15)N isotopic signatures. The bacterial community of A. hemprichii was rich and uneven, and diversity increased over time in all treatments. While distinct bacterial species were identified as a consequence of eutrophication, overfishing, or both, two bacterial species that could be classified to the genus Endozoicomonas were consistently abundant and constituted two thirds of bacteria in the coral. Several nitrogen-fixing and denitrifying bacteria were found in the coral specimens that were exposed to experimentally increased nutrients. However, no particular bacterial species was consistently associated with the coral under a given treatment and the single effects of manipulated eutrophication and overfishing could not predict the combined effect. Our data underlines the importance of conducting field studies in a holobiont framework, taking both, physiological and molecular measures into account.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.relation.urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0062091
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3632597/
dc.rightsArchived with thanks to PloS one
dc.subject.meshAnalysis of Variance
dc.subject.meshAnimals
dc.subject.meshAnthozoa
dc.subject.meshBacteria
dc.subject.meshBiodiversity
dc.subject.meshConservation of Natural Resources
dc.subject.meshEutrophication
dc.subject.meshFisheries
dc.subject.meshGammaproteobacteria
dc.subject.meshHigh-Throughput Nucleotide Sequencing
dc.subject.meshIndian Ocean
dc.subject.meshMolecular Typing
dc.subject.meshNitrogen Fixation
dc.subject.meshPhylogeny
dc.subject.meshPrincipal Component Analysis
dc.subject.meshRNA, Bacterial
dc.subject.meshRNA, Ribosomal, 16S
dc.subject.meshSequence Analysis, RNA
dc.subject.meshAnalysis of Variance
dc.subject.meshAnimals
dc.subject.meshAnthozoa
dc.subject.meshBacteria
dc.subject.meshBiodiversity
dc.subject.meshConservation of Natural Resources
dc.subject.meshEutrophication
dc.subject.meshFisheries
dc.subject.meshGammaproteobacteria
dc.subject.meshHigh-Throughput Nucleotide Sequencing
dc.subject.meshIndian Ocean
dc.subject.meshMolecular Typing
dc.subject.meshNitrogen Fixation
dc.subject.meshPhylogeny
dc.subject.meshPrincipal Component Analysis
dc.subject.meshRNA, Bacterial
dc.subject.meshRNA, Ribosomal, 16S
dc.subject.meshSequence Analysis, RNA
dc.titleIn-situ effects of eutrophication and overfishing on physiology and bacterial diversity of the red sea coral Acropora hemprichii.
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentReef Genomics Lab
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC3632597
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionCoral Reef Ecology Group (CORE), Leibniz Center for Tropical Marine Ecology (ZMT), Bremen, Germany.
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personBayer, Till
kaust.personRoder, Cornelia
kaust.personAranda, Manuel
kaust.personVoolstra, Christian R.
kaust.personLizcano, Javier
refterms.dateFOA2018-06-13T14:32:50Z


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Article - PLoS ONE - In-situ Effects of Eutrophication and Overfishing on Physiology and Bacterial Diversity of the Red Sea Coral Acropora hemprichii - 2011.pdf
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Supplemental File 1 - Figure S1 - Study site. Right panel shows position of the study area in the Red Sea. The circle on the left panel indicates study site at the Northern tip of Al Fahal-reef about 13 km off the Saudi-Arabian coast.
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Supplemental File 2 - Figure S2 - Treatment scheme. Coral fingers were attached to stainless steel screws on PVC frames with cable ties. A: cage treatment imitating overfishing pressures, frame with coral fragments underneath. B: fertilizer treatment imitating eutrophication pressures with slow releasing fertilizer diffusing out of the red bars. C: combined treatment of cage & fertilizer as in A and B.
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Supplemental File 3 - Figure S3 - Physiological parameters of corals and zooxanthellae. The left column shows the comparisons of treatments, and the right column the comparisons of coral colonies. Given P-values are from a 2-factorial ANOVA. See Table 1 for full results. Abbreviations used: Tr = Treatment, Ti = Time, G = Genotype. Asterisks indicate significant differences between two groups.
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Supplemental File 4 - Figure S4 - DGGE banding pattern of ITS2 from all coral samples. Please note that the coral samples are not depicted in a temporal order.
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Supplemental File 5 - Table S1 - Overview over sequence counts, taxonomic classification, and 16S reference amplicon sequence for all OTUs identified.

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