Symbiodinium transcriptomes: genome insights into the dinoflagellate symbionts of reef-building corals.
Name:
Article - PLoS ONE - Symbiodinium transcriptomes- Genome insights into the dinoflagellate symbionts of reef-building corals - 2012.pdf
Size:
535.1Kb
Format:
PDF
Description:
Article - Full Text
Type
ArticleAuthors
Bayer, TillAranda, Manuel

Sunagawa, Shinichi
Yum, Lauren
Desalvo, Michael K
Lindquist, Erika
Coffroth, Mary Alice
Voolstra, Christian R.

Medina, Mónica
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionMarine Science Program
Red Sea Research Center (RSRC)
Reef Genomics Lab
Date
2012-04-18Permanent link to this record
http://hdl.handle.net/10754/323516
Metadata
Show full item recordAbstract
Dinoflagellates are unicellular algae that are ubiquitously abundant in aquatic environments. Species of the genus Symbiodinium form symbiotic relationships with reef-building corals and other marine invertebrates. Despite their ecologic importance, little is known about the genetics of dinoflagellates in general and Symbiodinium in particular. Here, we used 454 sequencing to generate transcriptome data from two Symbiodinium species from different clades (clade A and clade B). With more than 56,000 assembled sequences per species, these data represent the largest transcriptomic resource for dinoflagellates to date. Our results corroborate previous observations that dinoflagellates possess the complete nucleosome machinery. We found a complete set of core histones as well as several H3 variants and H2A.Z in one species. Furthermore, transcriptome analysis points toward a low number of transcription factors in Symbiodinium spp. that also differ in the distribution of DNA-binding domains relative to other eukaryotes. In particular the cold shock domain was predominant among transcription factors. Additionally, we found a high number of antioxidative genes in comparison to non-symbiotic but evolutionary related organisms. These findings might be of relevance in the context of the role that Symbiodinium spp. play as coral symbionts.Our data represent the most comprehensive dinoflagellate EST data set to date. This study provides a comprehensive resource to further analyze the genetic makeup, metabolic capacities, and gene repertoire of Symbiodinium and dinoflagellates. Overall, our findings indicate that Symbiodinium possesses some unique characteristics, in particular the transcriptional regulation in Symbiodinium may differ from the currently known mechanisms of eukaryotic gene regulation.Citation
Bayer T, Aranda M, Sunagawa S, Yum LK, DeSalvo MK, et al. (2012) Symbiodinium Transcriptomes: Genome Insights into the Dinoflagellate Symbionts of Reef-Building Corals. PLoS ONE 7: e35269. doi:10.1371/journal.pone.0035269.Sponsors
This study was supported through NSF (National Science Foundation) awards IOS 0644438 and IOS 0926906 (MM), OCE 0424994 (MAC), a KAUST AEA (King Abdullah University of Science and Technology) 3 Joint Collaborative Research award (CRV), and through a Collaborative Travel Fund to TB made by King Abdullah University of Science and Technology. The work conducted by the U.S. Department of Energy Joint Genome Institute is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Publisher
Public Library of Science (PLoS)Journal
PLoS ONEPubMed ID
22529998PubMed Central ID
PMC3329448Additional Links
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0035269http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329448/
ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0035269
Scopus Count
Related articles
- Protein evolution in two co-occurring types of Symbiodinium: an exploration into the genetic basis of thermal tolerance in Symbiodinium clade D.
- Authors: Ladner JT, Barshis DJ, Palumbi SR
- Issue date: 2012 Nov 12
- Unfolding the secrets of coral-algal symbiosis.
- Authors: Rosic N, Ling EY, Chan CK, Lee HC, Kaniewska P, Edwards D, Dove S, Hoegh-Guldberg O
- Issue date: 2015 Mar 17
- Gene Expression Variation Resolves Species and Individual Strains among Coral-Associated Dinoflagellates within the Genus Symbiodinium.
- Authors: Parkinson JE, Baumgarten S, Michell CT, Baums IB, LaJeunesse TC, Voolstra CR
- Issue date: 2016 Feb 11
- Whole-Genome Transcriptome Analyses of Native Symbionts Reveal Host Coral Genomic Novelties for Establishing Coral-Algae Symbioses.
- Authors: Yoshioka Y, Yamashita H, Suzuki G, Zayasu Y, Tada I, Kanda M, Satoh N, Shoguchi E, Shinzato C
- Issue date: 2021 Jan 7
- Evolutionary analysis of orthologous cDNA sequences from cultured and symbiotic dinoflagellate symbionts of reef-building corals (Dinophyceae: Symbiodinium).
- Authors: Voolstra CR, Sunagawa S, Schwarz JA, Coffroth MA, Yellowlees D, Leggat W, Medina M
- Issue date: 2009 Jun