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dc.contributor.authorKodzius, Rimantas
dc.contributor.authorCastro, David
dc.contributor.authorFoulds, Ian G.
dc.contributor.authorParameswaran, Ash M.
dc.contributor.authorSumanpreet, K. Chhina
dc.date.accessioned2013-10-30T06:05:22Z
dc.date.available2013-10-30T06:05:22Z
dc.date.issued2013-01-21
dc.identifier.urihttp://hdl.handle.net/10754/304731
dc.description.abstractWe report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling the macroscopic observation. Such tests are most often used to explore antibody-antigen reactions. Agglutination has been used for protein assays using a biotin/streptavidin system as well as a hybridization based assay. The agglutination systems are prone to selftermination of the linking analyte, prone to active site saturation and loss of agglomeration at high analyte concentrations. We investigated the molecular target/ligand interaction, explaining the common agglutination problems related to analyte self-termination, linkage of the analyte to the same bead instead of different microbeads. We classified the agglutination process into three kinds of assays: a two- component assay, a three-component assay and a stepped three- component assay. Although we compared these three kinds of assays for recognizing DNA and protein molecules, the assay can be used for virtually any molecule, including ions and metabolites. In total, the optimized assay permits detecting analytes with high sensitivity in a short time, 5 min, at room temperature. Such a system is appropriate for POC testing.
dc.language.isoen
dc.publisherKAUST Winter Enrichment Program (WEP) 2013
dc.relation.urlhttp://www.acadox.com/class/1064#info
dc.subjectAgglutination
dc.subjectMicrobeads
dc.subjectDNA detection
dc.subjectMicrofluidics
dc.subjectDNA hybridization
dc.titleMicrobead agglutination based assays
dc.typePoster
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalKAUST Winter Enrichment Program (WEP) 2013
dc.eprint.versionPublisher's Version/PDF
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
refterms.dateFOA2018-06-14T06:34:14Z


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