Apparatus, System and Method for Fast Detection of Genetic Information by PCR in an Interchangeable Chip
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20110303_KAUS.P0043US.P1 - 03_03_11 Efiled Provisional Appl._ EFS Fee & Ack. Receipts (2).pdf
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Patent, Apparatus, System and Method for Fast Detection of Genetic Information by PCR in an Interchangeable Chip
Type
PatentPatent Status
Provisional ApplicationKAUST Department
Computational Bioscience Research Center (CBRC)Date
2011-03-03Submitted Date
2011-03-03Permanent link to this record
http://hdl.handle.net/10754/304710
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A polymerase chain reaction (PCR) device for fast amplification and detection of DNA includes an interchangeable PCR chamber, a temperature control component, and an optical detection system. The DNA amplification is performed on an interchangeable chip with volumes as small as 1.25 µl, while the heating and cooling rate may be as fast as 12.7 °C/second ensuring that the total time needed of only 25 minutes to complete the 35 cycle PCR amplification. The PCR may be performed according to a two-temperature approach for denaturing and annealing (Td and Ta) of DNA with the PCR chip, with which the amplification of male-specific SRY gene marker by utilizing raw saliva may be achieved. The genetic identification may be in-situ detected after PCR by the optical detection system.The following license files are associated with this item: