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dc.contributor.authorKodzius, Rimantas
dc.contributor.authorChang, Donald Choy
dc.contributor.authorGong, Xiuqing
dc.contributor.authorWen, Weijia
dc.contributor.authorWu, Jinbo
dc.contributor.authorXiao, Kang
dc.contributor.authorYi, Xin
dc.date.accessioned2013-10-30T06:09:42Z
dc.date.available2013-10-30T06:09:42Z
dc.date.issued2010-04-23
dc.identifier.urihttp://hdl.handle.net/10754/304707
dc.description.abstractAs part of the current miniaturization trend, biological reactions and processes are being adapted to microfluidics devices. PCR is the primary method employed in DNA amplification, its miniaturization is central to efforts to develop portable devices for diagnostics and testing purposes. A problem is the PCR-inhibitory effect due to interaction between PCR reagents and the surrounding environment, which effect is increased in high-surface-are-to-volume ration microfluidics. In this study, we evaluated the biocompatibility of various common materials employed in the fabrication of microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most of the cases, addition of bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, whereas they did show noticeable interaction with the DNA polymerase. Our test, instead of using microfluidic devices, can be easily conducted in common PCR tubes using a standard bench thermocycler. Our data supports an overview of the means by which the materials most bio-friendly to microfluidics can be selected.
dc.description.sponsorshipHong Kong Research Grants Council (Grant No. HKUST 603208 and 660207); Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST)
dc.language.isoen
dc.publisherHong Kong University of Science and Technology
dc.relation.urlhttp://pgworkshop.ust.hk/
dc.subjectPolymerase chain reaction (PCR)
dc.subjectDNA amplification
dc.subjectPCR compatibility
dc.subjectBiocompatibility
dc.titleMaterial Biocompatibility for PCR Microfluidic Chips
dc.typePoster
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalHong Kong University of Science and Technology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionThe Hong Kong University of Science and Technology (HKUST)
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
refterms.dateFOA2018-06-14T06:36:59Z


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