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dc.contributor.authorGong, Xiuqing
dc.contributor.authorYi, Xin
dc.contributor.authorXiao, Kang
dc.contributor.authorLi, Shunbo
dc.contributor.authorKodzius, Rimantas
dc.contributor.authorQin, Jianhua
dc.contributor.authorWen, Weijia
dc.date.accessioned2013-10-10T07:56:57Z
dc.date.available2013-10-10T07:56:57Z
dc.date.issued2010-08-05
dc.identifier.citationGong X, Yi X, Xiao K, Li S, Kodzius R, et al. (2010) Wax-bonding 3D microfluidic chips. Lab Chip 10: 2622. doi:10.1039/c004744a.
dc.identifier.issn1473-0197
dc.identifier.issn1473-0189
dc.identifier.doi10.1039/c004744a
dc.identifier.urihttp://hdl.handle.net/10754/303150
dc.description.abstractWe report a simple, low-cost and detachable microfluidic chip incorporating easily accessible paper, glass slides or other polymer films as the chip materials along with adhesive wax as the recycling bonding material. We use a laser to cut through the paper or film to form patterns and then sandwich the paper and film between glass sheets or polymer membranes . The hot-melt adhesive wax can realize bridge bonding between various materials, for example, paper, polymethylmethacrylate (PMMA) film, glass sheets, or metal plate. The bonding process is reversible and the wax is reusable through a melting and cooling process. With this process, a three-dimensional (3D) microfluidic chip is achievable by vacuating and venting the chip in a hot-water bath. To study the biocompatibility and applicability of the wax-based microfluidic chip, we tested the PCR compatibility with the chip materials first. Then we applied the wax-paper based microfluidic chip to HeLa cell electroporation (EP ). Subsequently, a prototype of a 5-layer 3D chip was fabricated by multilayer wax bonding. To check the sealing ability and the durability of the chip, green fluorescence protein (GFP) recombinant Escherichia coli (E. coli) bacteria were cultured, with which the chemotaxis of E. coli was studied in order to determine the influence of antibiotic ciprofloxacin concentration on the E. coli migration.
dc.description.sponsorshipAward No. SA-C0040/UK-C0016, made by King Abdullah University of Science and Technology (KAUST); Hong Kong RGC grants HKUST 603608; Nanoscience and Nanotechnology Program at HKUST
dc.language.isoen
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.urlhttp://xlink.rsc.org/?DOI=c004744a
dc.relation.urlhttp://pubs.rsc.org/en/content/articlepdf/2010/LC/C004744A
dc.relation.urlhttp://xlink.rsc.org/?DOI=c004744a
dc.rightsArchived with thanks to Lab on a Chip
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectMicrofluidic
dc.subjectBacteria
dc.subjectBiocompatibility
dc.subjectChip
dc.titleWax-bonding 3D microfluidic chips
dc.typeArticle
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalLab on a Chip
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionThe Hong Kong University of Science and Technology (HKUST)
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personKodzius, Rimantas
kaust.personWen, Weijia
kaust.grant.numberSA-C0040/UK-C0016
refterms.dateFOA2018-06-13T16:05:51Z
dc.date.published-online2010-08-05
dc.date.published-print2010


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