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dc.contributor.authorWu, Jinbo
dc.contributor.authorKodzius, Rimantas
dc.contributor.authorQin, Jianhua
dc.contributor.authorWen, Weijia
dc.contributor.authorXiao, Kang
dc.date.accessioned2013-10-10T08:57:40Z
dc.date.available2013-10-10T08:57:40Z
dc.date.issued2011-10-06
dc.identifier.citationWu J, Kodzius R, Xiao K, Qin J, Wen W (2011) Fast detection of genetic information by an optimized PCR in an interchangeable chip. Biomed Microdevices 14: 179-186. doi:10.1007/s10544-011-9595-6.
dc.identifier.issn1572-8781
dc.identifier.pmid21976029
dc.identifier.pmid21976029
dc.identifier.doi10.1007/s10544-011-9595-6
dc.identifier.urihttp://hdl.handle.net/10754/303136
dc.description.abstractIn this paper, we report the construction of a polymerase chain reaction (PCR) device for fast amplification and detection of DNA. This device consists of an interchangeable PCR chamber, a temperature control component as well as an optical detection system. The DNA amplification happens on an interchangeable chip with the volumes as low as 1.25 μl, while the heating and cooling rate was as fast as 12.7°C/second ensuring that the total time needed of only 25 min to complete the 35 cycle PCR amplification. An optimized PCR with two-temperature approach for denaturing and annealing (Td and Ta) of DNA was also formulated with the PCR chip, with which the amplification of male-specific sex determining region Y (SRY) gene marker by utilizing raw saliva was successfully achieved and the genetic identification was in-situ detected right after PCR by the optical detection system.
dc.description.sponsorshipAward No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST); Hong Kong Research Grants Council Grant No. HKUST 603608
dc.language.isoen
dc.publisherSpringer Nature
dc.rightsArchived with thanks to Biomedical microdevices
dc.subjectPoint-of-Care Testing
dc.subjectMolecular diagnostics
dc.subjectPolymerase chain reaction (PCR)
dc.subjectMicrofluidic Chip
dc.subjectGenetic identification
dc.subject.meshDNA
dc.subject.meshHumans
dc.subject.meshMicrofluidic Analytical Techniques
dc.subject.meshPoint-of-Care Systems
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshSaliva
dc.subject.meshSex-Determining Region Y Protein
dc.subject.meshDNA
dc.subject.meshHumans
dc.subject.meshMicrofluidic Analytical Techniques
dc.subject.meshPoint-of-Care Systems
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshSaliva
dc.subject.meshSex-Determining Region Y Protein
dc.titleFast detection of genetic information by an optimized PCR in an interchangeable chip.
dc.typeArticle
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalBiomedical Microdevices
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionNano Science and Nano Technology program and Physics Department, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
dc.contributor.institutionKAUST-HKUST Micro/Nanofluidic Joint Laboratory, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personKodzius, Rimantas
kaust.grant.numberSA-C0040/UK-C0016
refterms.dateFOA2018-06-13T14:07:08Z
dc.date.published-online2011-10-06
dc.date.published-print2012-02


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