Comparative Proteomic Profiling of Mycobacterium bovis and BCG Vaccine Strains
Type
ThesisAuthors
Gao, GeAdvisors
Pain, Arnab
Committee members
Gehring, Christoph A
Ravasi, Timothy

Program
BioscienceKAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionDate
2013-09Permanent link to this record
http://hdl.handle.net/10754/302495
Metadata
Show full item recordAbstract
BCG is the only licensed human vaccine currently available against TB. Derived from a virulent strain of M. bovis, the vaccine was thought to have struck a balance between reduced virulence and preserved immunogenicity. Nowadays, BCG vaccine strains used in different countries and vaccination programs show clear variations in their genomes and immune protective properties. The aim of this study was to characterize the proteomic profile on Mycobacterium bovis and five BCG strains Pasteur, Tokyo, Danish, Phipps and Birkhaug by Tandem Mass Tag® (TMT®)-labeling quantitative proteomic approach. In total, 420 proteins were identified and 377 of them were quantitated for their relative abundance. We reported the number and relationship of differential expressed proteins in BCG strains compared to M. bovis and investigated their functions by bioinformatics analysis. Several interesting up-regulated and down-regulated protein targets were found. The identified proteins and their quantitative expression profiles provide a basis for further understanding of the cellular biology of M. bovis and BCG vaccine strains, and hopefully would assist in the design of better anti-TB vaccine and drugs.Citation
Gao, G. (2013). Comparative Proteomic Profiling of Mycobacterium bovis and BCG Vaccine Strains. KAUST Research Repository. https://doi.org/10.25781/KAUST-G5ZF4ae974a485f413a2113503eed53cd6c53
10.25781/KAUST-G5ZF4